A strategy for multimodal canopy images registration

Registration of complex and self-similar images such as plant canopy images is a challenge in plant sciences. Yet, this is often a required step for multimodal imaging, where unaligned sensors yield unregistered image pairs. We propose a pipeline adapted to such constraints, applied to apple tree canopies. Specifically, we apply an intensity-based registration on downscaled and/or Gaussian blurred versions of the targeted images. This helps to eliminate spurious details, which smooths the optimization landscape and also helps to reduce differences between the modalities. Results show better registration than with standard feature-based or intensity-based methods

Simulations de données afin d'améliorer la segmentation automatique de la tavelure du pommier

National audienc

Evaluation of 3D/2D Imaging and Image Processing Techniques for the Monitoring of Seed Imbibition

Seed imbibition is a very important process in plant biology by which, thanks to a simple water income, a dry seed may turn into a developing organism. In natural conditions, this process occurs in the soil, e.g., with difficult access for a direct observation. Monitoring the seed imbibition with non-invasive imaging techniques is therefore an important and possibly challenging task if one tries to perform it in natural conditions. In this report, we describe a set of four different imaging techniques that enable to addressing this task either in 3D or in 2D. For each technique, the following items are proposed. A detailed experimental protocol is provided to acquire images of the imbibition process. With the illustration of real data, the significance of the physical quantities measured in terms of their relation to the income of water in the seed is presented. Complete image analysis pipelines are then proposed to extract dynamic information on the imbibition process from such monitoring experiments. A final discussion compares the advantages and current limitations of each technique in addition to elements concerning the associated throughput and cost. These are criteria especially relevant in the field of plant phenotyping where large populations of plants are imaged to produce quantitatively significative traits after image processin

The G-Quadruplex Ligand Telomestatin Impairs Binding of Topoisomerase IIIα to G-Quadruplex-Forming Oligonucleotides and Uncaps Telomeres in ALT Cells

In Alternative Lengthening of Telomeres (ALT) cell lines, specific nuclear bodies called APBs (ALT-associated PML bodies) concentrate telomeric DNA, shelterin components and recombination factors associated with telomere recombination. Topoisomerase IIIα (Topo III) is an essential telomeric-associated factor in ALT cells. We show here that the binding of Topo III to telomeric G-overhang is modulated by G-quadruplex formation. Topo III binding to G-quadruplex-forming oligonucleotides was strongly inhibited by telomestatin, a potent and specific G-quadruplex ligand. In ALT cells, telomestatin treatment resulted in the depletion of the Topo III/BLM/TRF2 complex and the disruption of APBs and led to the segregation of PML, shelterin components and Topo III. Interestingly, a DNA damage response was observed at telomeres in telomestatin-treated cells. These data indicate the importance of G-quadruplex stabilization during telomere maintenance in ALT cells. The function of TRF2/Topo III/BLM in the resolution of replication intermediates at telomeres is discussed

Data for: NOVEL DATA AUGMENTATION STRATEGIES TO BOOST SUPERVISED SEGMENTATION OF PLANT DISEASES

This is the unnannotated dataset of IR images of apple plants inoculated by scab used in this article. The annotated dataset will be made public further on

Data for: NOVEL DATA AUGMENTATION STRATEGIES TO BOOST SUPERVISED SEGMENTATION OF PLANT DISEASES

This is the unnannotated dataset of IR images of apple plants inoculated by scab used in this article. The annotated dataset will be made public further on.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Resistance to senescence induction and telomere shortening by a G-quadruplex ligand inhibitor of telomerase

International audienc

Resistance to senescence induction and telomere shortening by a G-quadruplex ligand inhibitor of telomerase

International audienc

Resistance to senescence induction and telomere shortening by a G-quadruplex ligand inhibitor of telomerase

International audienc