THE PENETRATION OF REOVIRUS RNA AND INITIATION OF ITS GENETIC FUNCTION IN L-STRAIN FIBROBLASTS

Reovirus type 3 is phagocytized by L cells and rapidly sequestered inside lysosomes. Hydrolases within these organelles are capable of stripping the viral coat proteins, but they fail to degrade the double-stranded RNA genome. These observations support the view that sojourn of reovirus in lysosomes, when the lytic enzymes uncoat its genome, is an obligatory step in the sequence of infection. Although the mechanism for transferring the uncoated RNA out of lysosomes remains to be elucidated, evidence is presented suggesting that progeny genomes are bound to site(s) possessing the fine structure of viral inclusions or factories. It appears that both the synthesis of single- and double-stranded viral RNA and the morphogenesis of progeny virus particles occur in such factories

Optical Identification of He White Dwarfs Orbiting Four Millisecond Pulsars in the Globular Cluster 47 Tucanae

We used ultra-deep UV observations obtained with the Hubble Space Telescope to search for optical companions to binary millisecond pulsars (MSPs) in the globular cluster 47 Tucanae. We identified four new counterparts (to MSPs 47TucQ, 47TucS, 47TucT and 47TucY) and confirmed those already known (to MSPs 47TucU and 47TucW). In the color magnitude diagram, the detected companions are located in a region between the main sequence and the CO white dwarf cooling sequences, consistent with the cooling tracks of He white dwarfs of mass between 0.15 Msun and 0.20 Msun. For each identified companion, mass, cooling age, temperature and pulsar mass (as a function of the inclination angle) have been derived and discussed. For 47TucU we also found that the past accretion history likely proceeded in a sub-Eddington rate. The companion to the redback 47TucW is confirmed to be a non degenerate star, with properties particularly similar to those observed for black widow systems. Two stars have been identified within the 2-sigma astrometric uncertainty from the radio positions of 47TucH and 47TucI, but the available data prevent us from firmly assessing whether they are the true companions of these two MSPs.Comment: 27 pages, 7 figures, Accepted for publication by Ap

Radio Timing and Optical Photometry of the Black Widow System PSR J1953+1846A in the Globular Cluster M71

We report on the determination of the astrometric, spin and orbital parameters for PSR J1953+1846A, a "black widow" binary millisecond pulsar in the globular cluster M71. By using the accurate position and orbital parameters obtained from radio timing, we identified the optical companion in ACS/Hubble Space Telescope images. It turns out to be a faint (m_F606W>=24, m_F814W>=23) and variable star located at only ~0.06" from the pulsar timing position. The light curve shows a maximum at the pulsar inferior conjunction and a minimum at the pulsar superior conjunction, thus confirming the association with the system. The shape of the optical modulation suggests that the companion star is heated, likely by the pulsar wind. The comparison with the X-ray light curve possibly suggests the presence of an intra-binary shock due to the interaction between the pulsar wind and the material released by the companion. This is the second identification (after COM-M5C) of an optical companion to a black widow pulsar in a globular cluster. Interestingly, the two companions show a similar light curve and share the same position in the color magnitude diagram.Comment: Accepted for publication by ApJ; 33 Pages, 10 Figures, 3 Table