Growth performance, morphometric analysis of the intestinal mucosa and thyroid of broiler fed canola meal.

Abstract: The aim of this study was to evaluate the effect of replacing soybean meal with canola meal in broiler diets on performance, liver histopathology, morphometry of the intestinal mucosa and thyroid. One-day-old Cobb chicks (n=300) were distributed in a completely randomized design, with increasing levels of replacement of soybean meal with canola meal (0, 25, 50, 75 and 100%) and 6 repetitions of 10 birds each. Weight gain decreased linearly (P< 0.05) with increasing levels of inclusion of canola meal, in all stages. Villus height in the duodenum and jejunum linearly decreased (P< 0.05). Follicle diameter and thyroid follicular epithelium height increased linearly with increasing levels of canola meal (P< 0.05). Similarly, there was an increase in relative weight of liver and heart, and liver steatosis in the highest levels of replacement. In conclusion, the replacement of soybean meal with canola meal can reduce performance, adversely affecting the thyroid, liver and the morphometric characteristics in the duodenum and jejunum. Resumo: O objetivo deste estudo foi avaliar o efeito da substituição do farelo de soja pelo farelo de canola na dieta de frangos de corte sobre o desempenho, a histopatologia hepática, a morfometria da mucosa intestinal e da tiroide. Foram distribuídos 300 pintos com um dia de idade em um delineamento inteiramente causualizado, com níveis crescentes de substituição do farelo de soja pelo farelo de canola (0, 25, 50, 75 e 100%), 6 repetições com 10 aves cada. O ganho de peso diminuiu linearmente (P< 0,05) com níveis crescentes da substituição, em todas as fases. Assim como, a altura das vilosidades no duodeno e jejuno diminuiu linearmente (P< 0,05) em todas as idades avaliadas. O diâmetro do folículo e a altura do epitélio da tireoide aumentaram linearmente conforme aumentou os níveis de farelo de canola (P< 0,05). Da mesma forma, houve um aumento do peso relativo do fígado e do coração, e aparecimento de esteatose hepática nos níveis mais altos de substituição. Em conclusão, a substituição do farelo de soja pelo farelo de canola pode reduzir o desempenho, afetando negativamente a tireoide, o fígado e as características morfométricas no duodeno e jejuno

Criopolímero: toxidade a célula espermática de suínos.

Com o objetivo de avaliar a utilização do polímero PNVCL (N-vinilcaprolactama) quanto sua precipitação em temperaturas acima de LCST (temperatura crítica inferior de solubilização)e também sua toxidade quando em contato com sêmen suíno. O PNVCL foi utilizado nas seguintes concentrações: T1 = 5000 mg/L; T2 = 2500 mg/L; T3= 1250 mg/L; T4 = 625 mg/L; T5 = 312,5 mg/L; T6 = 156,3 mg/L; T7 = 78,1 mg/L; T8 = 39,1 mg/L e T9 = 0 mg/L. Quando colocado em contato com o sêmen os tratamentos foram constituídos de 500 &#956;L de sêmen e 500&#956;L do tratamento em questão. Os resultados demonstram que a utilização de concentrações elevadas do polímero acarreta a precipitação do mesmo prejudicando assim as variáveis de qualidade seminal. Entretanto o PNVCL não é tóxico em contato com a célula. Desta forma a utilização do polímero é viável, contudo em baixas concentrações

Evaluation of sperm quality snakes Erythrolamprus poecilogyrus sublineatus (Cope, 1860) (Serpentes, Dipsadidae)

Abstract Erythrolamprus poecilogyrus sublineatus (Cope, 1860), is a species widely distributed in the Pampa Domain, occurring in Rio Grande do Sul, Argentina and Uruguay, mainlyin the pampa region. In the coastal region of southern Brazil this is serpent is considered one of the most abundant. The purpose of the present study is to describe the techniques of sperm evaluation in vitro for E. poecilogyrus sublineatus in the coastal plain of Rio Grande do Sul, Brazil. After laparatomy the efferent vases were collected and the semen was diluted in 1ml Beltsville Thawing Solution. The characteristics of motility, membrane integrity, mitochondria, acrosome, DNA, cell viability and cellular functionality were evaluated. Fluorescent probes were used for the evaluation of sperm structure in epifluorescence microscope. With the techniques described, it was possible to identify intact and injured cells, enabling the determination of cell characteristics for the spring season (October and November). It was observed in the analyses that 80% of sperm cells were mobile and that 84.1 ± 8.0% of sperm membranes were intact. The standards found were of 48 ± 13.8% of intact acrosome, 73.6 ± 6.0 of perfect DNA and of 91.8 ± 4.0 of functional mitochondria. Thus, these values from the sperm analysis can be used as standards for the species Erythrolamprus poecilogyrus sublineatus

Reduce glutathione and atp in the seminal cryopreservation of tambaqui.

Made available in DSpace on 2019-01-28T23:44:17Z (GMT). No. of bitstreams: 1 Cryoletters.pdf: 158601 bytes, checksum: 686e22c0b4d64043f334a7704cd1e67f (MD5) Previous issue date: 2019-01-28bitstream/item/191419/1/Cryoletters.pd

Reduce glutathione and atp in the seminal cryopreservation of tambaqui.

Made available in DSpace on 2019-01-28T23:44:17Z (GMT). No. of bitstreams: 1 Cryoletters.pdf: 158601 bytes, checksum: 686e22c0b4d64043f334a7704cd1e67f (MD5) Previous issue date: 2019-01-28bitstream/item/191419/1/Cryoletters.pd

Sperm impairments in adult vesper mice (Calomys laucha) caused byin uteroexposure to bisphenol A

This study aimed to evaluate the effects of in utero administration of bisphenol A (BPA) on semen parameters of vesper mice. Sixty female Calomys laucha were divided into six groups and received by gavage during gestation the following substances: Water (negative control), Olive Oil (vehicle control), Diethylstilbestrol (DES – positive control – 6.5 μg kg−1 bw) and BPA (40, 80 and 200 μg kg−1 bw). Male offspring were euthanised at 70 days of age, and sperm parameters were analysed. BPA reduced normal sperm morphology (water = 96.1 ± 0.65; BPA200 = 96.8 ± 2.3%), sperm membrane integrity (water = 88.8 ± 1,65; BPA200 = 70.6 ± 4,15%), sperm motility (water = 87.5 ± 1.71; BPA200 = 51.3 ±9.9%) and in vitro penetration rates (water = 55.0 ± 7.14; BPA200 = 7.47 ±2.96%), but it did not affect body weight, anogenital distance, sperm DNA integrity and acrosome integrity. In conclusion, in utero exposure to BPA caused a reduction in sperm parameters of adult C. laucha. Natural mating studies should be conducted to verify the effects of BPA on fertility of the animals