Site-Specific Effects of PECAM-1 on Atherosclerosis in LDL Receptor-Deficient Mice

Objective—Atherosclerosis is a vascular disease that involves lesion formation at sites of disturbed flow under the influence of genetic and environmental factors. Endothelial expression of adhesion molecules that enable infiltration of immune cells is important for lesion development. Platelet/endothelial cell adhesion molecule-1 (PECAM-1; CD31) is an adhesion and signaling receptor expressed by many cells involved in atherosclerotic lesion development. PECAM-1 transduces signals required for proinflammatory adhesion molecule expression at atherosusceptible sites; thus, it is predicted to be proatherosclerotic. PECAM-1 also inhibits inflammatory responses, on which basis it is predicted to be atheroprotective. Methods and Results—We evaluated herein the effect of PECAM-1 deficiency on development of atherosclerosis in LDL receptor– deficient mice. We found that PECAM-1 has both proatherosclerotic and atheroprotective effects, but that the former dominate in the inner curvature of the aortic arch whereas the latter dominate in the aortic sinus, branching arteries, and descending aorta. Endothelial cell expression of PECAM-1 was sufficient for its atheroprotective effects in the aortic sinus but not in the descending aorta, where the atheroprotective effects of PECAM-1 also required its expression on bone marrow–derived cells. Conclusion—We conclude that PECAM-1 influences initiation and progression of atherosclerosis both positively and negatively, and that it does so in a site-specific manner. (Arterioscler Thromb Vasc Biol. 2008;28:1996-2002

Anti-Hispanic prejudice drives opposition to immigration in the U.S.

Last June, the U.S. Senate passed a comprehensive immigration bill that would give legal status to 11 million undocumented migrants, but this bill has remained stalled in the House of Representatives, reflecting the anti-immigration sentiments of many Americans. New research from Todd K. Hartman, Benjamin J. Newman, and C. Scott Bell investigates why so many are against immigration reform, and the role played by racial prejudice against Hispanics. Using surveys designed to detect anti-Hispanic prejudice, they find that white Americans react very differently to law-violating behaviors whether they are committed by Hispanic immigrants or not, suggesting a significant and persistent bias against them

Kiloparsec-scale Spatial Offsets in Double-peaked Narrow-line Active Galactic Nuclei. I. Markers for Selection of Compelling Dual Active Galactic Nucleus Candidates

Merger-remnant galaxies with kpc-scale separation dual active galactic nuclei (AGNs) should be widespread as a consequence of galaxy mergers and triggered gas accretion onto supermassive black holes, yet very few dual AGNs have been observed. Galaxies with double-peaked narrow AGN emission lines in the Sloan Digital Sky Survey are plausible dual AGN candidates, but their double-peaked profiles could also be the result of gas kinematics or AGN-driven outflows and jets on small or large scales. To help distinguish between these scenarios, we have obtained spatial profiles of the AGN emission via follow-up long-slit spectroscopy of 81 double-peaked narrow-line AGNs in SDSS at 0.03 < z < 0.36 using Lick, Palomar, and MMT Observatories. We find that all 81 systems exhibit double AGN emission components with ~kpc projected spatial separations on the sky, which suggests that they are produced by kpc-scale dual AGNs or kpc-scale outflows, jets, or rotating gaseous disks. In addition, we find that the subsample (58%) of the objects with spatially compact emission components may be preferentially produced by dual AGNs, while the subsample (42%) with spatially extended emission components may be preferentially produced by AGN outflows. We also find that for 32% of the sample the two AGN emission components are preferentially aligned with the host galaxy major axis, as expected for dual AGNs orbiting in the host galaxy potential. Our results both narrow the list of possible physical mechanisms producing the double AGN components, and suggest several observational criteria for selecting the most promising dual AGN candidates from the full sample of double-peaked narrow-line AGNs. Using these criteria, we determine the 17 most compelling dual AGN candidates in our sample.Comment: 12 pages, 8 figures, published in ApJ. Modified from original version to reflect referee's comment

The DEEP2 Redshift Survey: Lyman Alpha Emitters in the Spectroscopic Database

We present the first results of a search for Lyman-alpha emitters (LAEs) in the DEEP2 spectroscopic database that uses a search technique that is different from but complementary to traditional narrowband imaging surveys. We have visually inspected ~20% of the available DEEP2 spectroscopic data and have found nine high-quality LAEs with clearly asymmetric line profiles and an additional ten objects of lower quality, some of which may also be LAEs. Our survey is most sensitive to LAEs at z=4.4-4.9 and that is indeed where all but one of our high-quality objects are found. We find the number density of our spectroscopically-discovered LAEs to be consistent with those found in narrowband imaging searches. The combined, averaged spectrum of our nine high-quality objects is well fit by a two-component model, with a second, lower-amplitude component redshifted by ~420 km/s with respect to the primary Lyman-alpha line, consistent with large-scale outflows from these objects. We conclude by discussing the advantages and future prospects of blank-sky spectroscopic surveys for high-z LAEs.Comment: Accepted for publication in Ap

Photoactivatable genetically encoded calcium indicators for targeted neuronal imaging.

Circuit mapping requires knowledge of both structural and functional connectivity between cells. Although optical tools have been made to assess either the morphology and projections of neurons or their activity and functional connections, few probes integrate this information. We have generated a family of photoactivatable genetically encoded Ca(2+) indicators that combines attributes of high-contrast photolabeling with high-sensitivity Ca(2+) detection in a single-color protein sensor. We demonstrated in cultured neurons and in fruit fly and zebrafish larvae how single cells could be selected out of dense populations for visualization of morphology and high signal-to-noise measurements of activity, synaptic transmission and connectivity. Our design strategy is transferrable to other sensors based on circularly permutated GFP (cpGFP)

On the Evolution of the Velocity-Mass-Size Relations of Disk-Dominated Galaxies over the Past 10 Billion Years

We study the evolution of the scaling relations between maximum circular velocity, stellar mass and optical half-light radius of star-forming disk-dominated galaxies in the context of LCDM-based galaxy formation models. Using data from the literature combined with new data from the DEEP2 and AEGIS surveys we show that there is a consistent observational and theoretical picture for the evolution of these scaling relations from z\sim 2 to z=0. The evolution of the observed stellar scaling relations is weaker than that of the virial scaling relations of dark matter haloes, which can be reproduced, both qualitatively and quantitatively, with a simple, cosmologically-motivated model for disk evolution inside growing NFW dark matter haloes. In this model optical half-light radii are smaller, both at fixed stellar mass and maximum circular velocity, at higher redshifts. This model also predicts that the scaling relations between baryonic quantities evolve even more weakly than the corresponding stellar relations. We emphasize, though, that this weak evolution does not imply that individual galaxies evolve weakly. On the contrary, individual galaxies grow strongly in mass, size and velocity, but in such a way that they move largely along the scaling relations. Finally, recent observations have claimed surprisingly large sizes for a number of star-forming disk galaxies at z \sim 2, which has caused some authors to suggest that high redshift disk galaxies have abnormally high spin parameters. However, we argue that the disk scale lengths in question have been systematically overestimated by a factor \sim 2, and that there is an offset of a factor \sim 1.4 between H\alpha sizes and optical sizes. Taking these effects into account, there is no indication that star forming galaxies at high redshifts (z\sim 2) have abnormally high spin parameters.Comment: 19 pages, 10 figures, accepted to MNRAS, minor changes to previous versio

The DEEP2 Galaxy Redshift Survey: Mean Ages and Metallicities of Red Field Galaxies at z ~ 0.9 from Stacked Keck/DEIMOS Spectra

As part of the DEEP2 galaxy redshift survey, we analyze absorption line strengths in stacked Keck/DEIMOS spectra of red field galaxies with weak to no emission lines, at redshifts 0.7 <= z <= 1. Comparison with models of stellar population synthesis shows that red galaxies at z ~ 0.9 have mean luminosity-weighted ages of the order of only 1 Gyr and at least solar metallicities. This result cannot be reconciled with a scenario where all stars evolved passively after forming at very high z. Rather, a significant fraction of stars can be no more than 1 Gyr old, which means that star formation continued to at least z ~ 1.2. Furthermore, a comparison of these distant galaxies with a local SDSS sample, using stellar populations synthesis models, shows that the drop in the equivalent width of Hdelta from z ~ 0.9 to 0.1 is less than predicted by passively evolving models. This admits of two interpretations: either each individual galaxy experiences continuing low-level star formation, or the red-sequence galaxy population from z ~ 0.9 to 0.1 is continually being added to by new galaxies with younger stars.Comment: A few typos were corrected and numbers in Table 1 were revise

Dependence of Galaxy Quenching on Halo Mass and Distance from its Centre

We study the dependence of star-formation quenching on galaxy mass and environment, in the SDSS (z~0.1) and the AEGIS (z~1). It is crucial that we define quenching by low star-formation rate rather than by red colour, given that one third of the red galaxies are star forming. We address stellar mass M*, halo mass Mh, density over the nearest N neighbours deltaN, and distance to the halo centre D. The fraction of quenched galaxies appears more strongly correlated with Mh at fixed M* than with M* at fixed Mh, while for satellites quenching also depends on D. We present the M*-Mh relation for centrals at z~1. At z~1, the dependence of quenching on M* at fixed Mh is somewhat more pronounced than at z~0, but the quenched fraction is low (10%) and the haloes are less massive. For satellites, M*-dependent quenching is noticeable at high D, suggesting a quenching dependence on sub-halo mass for recently captured satellites. At small D, where satellites likely fell in more than a few Gyr ago, quenching strongly depends on Mh, and not on M*. The Mh-dependence of quenching is consistent with theoretical wisdom where virial shock heating in massive haloes shuts down accretion and triggers ram-pressure stripping, causing quenching. The interpretation of deltaN is complicated by the fact that it depends on the number of observed group members compared to N, motivating the use of D as a better measure of local environment.Comment: 23 pages, 13 figures, accepted by MNRA