Occupational Exposure to Mycotoxins: Current Knowledge and Prospects.

Occupational exposure to mycotoxins is supposedly very frequent, but it is rarely reported in the scientific literature. Several recent studies described occupational exposure to the aflatoxin B1 (AFB1) mycotoxin in different occupational settings. Previously, exposure to other mycotoxins was shown in the animal husbandry and food processing sectors, confirming that occupational exposure cannot be negligible. However, no guidelines or standard methodologies are available for helping occupational hygienists to consider mycotoxin exposure in their interventions. This article reviews the literature on this problem and recommends some actions for the better management of this risk factor in occupational settings, especially where environmental conditions are favorable to fungal presence

Comparison of indoor and outdoor fungi and particles in poultry units

A descriptive study was developed in order to compare indoor and outdoor air contamination caused by fungi and particles in seven poultry units. Twenty eight air samples of 25 litters were collected through the impaction method on malt extract agar. Air sampling and particles concentration measurement were done in the interior and also outside premises of the poultries’ pavilions. Regarding the fungal load in the air, indoor concentration of mold was higher than outside air in six poultry units. Twenty eight species / genera of fungi were identified indoor, being Scopulariopsis brevicaulis (40.5%) the most commonly isolated species and Rhizopus sp. (30.0%) the most commonly isolated genus. Concerning outdoor, eighteen species/genera of fungi were isolated, being Scopulariopsis brevicaulis (62.6%) also the most isolated. All the poultry farms analyzed presented indoor fungi different from the ones identified outdoors. Regarding particles’ contamination, PM2.5, PM5.0 and PM10 had a statistically significant difference (Mann-Whitney U test) between the inside and outside of the pavilions, with the inside more contaminated (p=.006; p=.005; p=.005, respectively). The analyzed poultry units are potential reservoirs of substantial amounts of fungi and particles and could therefore free them in the atmospheric air. The developed study showed that indoor air was more contaminated than outdoors, and this can result in emission of potentially pathogenic fungi and particles via aerosols from poultry units to the environment, which may post a considerable risk to public health and contribute to environmental pollution

Accessing occupational exposure to fungi in a cork industry

In this study we aimed to access fungal exposure in workers from one cork industry through the mycological analysis of their nasal exudate and the environmental fungal contamination of their surroundings as well. Nasal mucous samples from 127 workers were taken with sterilized cotton swabs.The fungal species identified in the collected nose swabs were shown to be correlated with the results obtained in the environment. Eighty workers (63.0%) presented contamination of their nose nostril with Chrysonilia sitophila, which number of colonies was countless. Within the Aspergillus genus, the complexes Fumigati, Circumdati, Versicolores and Candidi were isolated. No azole-resistant Aspergillus isolates grew in the selective media used (screened itraconazole and voriconazole resistance).This approach allowed us to estimate the risk associated with these tasks performance. Moreover, the cork industry is related to high dust contamination and this can promote exposure to fungi since dust particles can act as carriers of fungi to the worker’s nose. Assessment by molecular tools will ensure the specific targeting of DNA from P. glabrum complex in workers nose

Temperature Fluctuations and Abundances in HII Galaxies

There is evidence for temperature fluctuations in Planetary Nebulae and in Galactic HII regions. If such fluctuations occur in the low-metallicity, extragalactic HII regions used to probe the primordial helium abundance, the derived 4He mass fraction, Y_P, could be systematically different from the true primordial value. For cooler, mainly high-metallicity HII regions the derived helium abundance may be nearly unchanged but the oxygen abundance could have been seriously underestimated. For hotter, mainly low-metallicity HII regions the oxygen abundance is likely accurate but the helium abundance could be underestimated. The net effect is to tilt the Y vs. Z relation, making it flatter and resulting in a higher inferred Y_P. Although this effect could be large, there are no data which allow us to estimate the size of the temperature fluctuations for the extragalactic HII regions. Therefore, we have explored this effect via Monte Carlos in which the abundances derived from a fiducial data set are modified by \Delta-T chosen from a distribution with 0 < \Delta-T < \Delta-T_max where \Delta-T_max is varied from 500K to 4000K. It is interesting that although this effect shifts the locations of the HII regions in Y vs. O/H plane, it does not introduce any significant additional dispersion.Comment: 11 pages, 9 postscript figures; submitted to the Ap