2,776 research outputs found
Time--Distance Helioseismology Data Analysis Pipeline for Helioseismic and Magnetic Imager onboard Solar Dynamics Observatory (SDO/HMI) and Its Initial Results
The Helioseismic and Magnetic Imager onboard the Solar Dynamics Observatory
(SDO/HMI) provides continuous full-disk observations of solar oscillations. We
develop a data-analysis pipeline based on the time-distance helioseismology
method to measure acoustic travel times using HMI Doppler-shift observations,
and infer solar interior properties by inverting these measurements. The
pipeline is used for routine production of near-real-time full-disk maps of
subsurface wave-speed perturbations and horizontal flow velocities for depths
ranging from 0 to 20 Mm, every eight hours. In addition, Carrington synoptic
maps for the subsurface properties are made from these full-disk maps. The
pipeline can also be used for selected target areas and time periods. We
explain details of the pipeline organization and procedures, including
processing of the HMI Doppler observations, measurements of the travel times,
inversions, and constructions of the full-disk and synoptic maps. Some initial
results from the pipeline, including full-disk flow maps, sunspot subsurface
flow fields, and the interior rotation and meridional flow speeds, are
presented.Comment: Accepted by Solar Physics topical issue 'Solar Dynamics Observatory
Physical Properties of Wave Motion in Inclined Magnetic Fields Within Sunspot Penumbrae
At the surface of the Sun, acoustic waves appear to be affected by the
presence of strong magnetic fields in active regions. We explore the
possibility that the inclined magnetic field in sunspot penumbrae may convert
primarily vertically propagating acoustic waves into elliptical motion. We use
helioseismic holography to measure the modulus and phase of the correlation
between incoming acoustic waves and the local surface motion within two
sunspots. These correlations are modeled assuming the surface motion is
elliptical, and we explore the properties of the elliptical motion on the
magnetic field inclination. We also demonstrate that the phase shift of the
outward propagating waves is opposite to the phase shift of the inward
propagating waves in stronger, more vertical fields, but similar to the inward
phase shifts in weaker, more inclined fields.Comment: 22 pages, 13 figure
Surface-focused Seismic Holography of Sunspots: I. Observations
We present a comprehensive set of observations of the interaction of p-mode
oscillations with sunspots using surface-focused seismic holography. Maps of
travel-time shifts, relative to quiet-Sun travel times, are shown for incoming
and outgoing p modes as well as their mean and difference. We compare results
using phase-speed filters with results obtained with filters that isolate
single p-mode ridges, and further divide the data into multiple temporal
frequency bandpasses. The f mode is removed from the data. The variations of
the resulting travel-time shifts with magnetic-field strength and with the
filter parameters are explored. We find that spatial averages of these shifts
within sunspot umbrae, penumbrae, and surrounding plage often show strong
frequency variations at fixed phase speed. In addition, we find that positive
values of the mean and difference travel-time shifts appear exclusively in
waves observed with phase-speed filters that are dominated by power in the
low-frequency wing of the p1 ridge. We assess the ratio of incoming to outgoing
p-mode power using the ridge filters and compare surface-focused holography
measurements with the results of earlier published p-mode scattering
measurements using Fourier-Hankel decomposition.Comment: Solar Physics, accepte
Helioseismology of Sunspots: A Case Study of NOAA Region 9787
Various methods of helioseismology are used to study the subsurface
properties of the sunspot in NOAA Active Region 9787. This sunspot was chosen
because it is axisymmetric, shows little evolution during 20-28 January 2002,
and was observed continuously by the MDI/SOHO instrument. (...) Wave travel
times and mode frequencies are affected by the sunspot. In most cases, wave
packets that propagate through the sunspot have reduced travel times. At short
travel distances, however, the sign of the travel-time shifts appears to depend
sensitively on how the data are processed and, in particular, on filtering in
frequency-wavenumber space. We carry out two linear inversions for wave speed:
one using travel-times and phase-speed filters and the other one using mode
frequencies from ring analysis. These two inversions give subsurface wave-speed
profiles with opposite signs and different amplitudes. (...) From this study of
AR9787, we conclude that we are currently unable to provide a unified
description of the subsurface structure and dynamics of the sunspot.Comment: 28 pages, 18 figure
The TcEG1 beetle (Tribolium castaneum) cellulase produced in transgenic switchgrass is active at alkaline pH and auto-hydrolyzes biomass for increased cellobiose release
Background
Genetically engineered biofuel crops, such as switchgrass (Panicum virgatum L.), that produce their own cell wall-digesting cellulase enzymes would reduce costs of cellulosic biofuel production. To date, non-bioenergy plant models have been used in nearly all studies assessing the synthesis and activity of plant-produced fungal and bacterial cellulases. One potential source for cellulolytic enzyme genes is herbivorous insects adapted to digest plant cell walls. Here we examine the potential of transgenic switchgrass-produced TcEG1 cellulase from Tribolium castaneum (red flour beetle). This enzyme, when overproduced in Escherichia coliand Saccharomyces cerevisiae, efficiently digests cellulose at optima of 50 °C and pH 12.0. Results TcEG1 that was produced in green transgenic switchgrass tissue had a range of endoglucanase activity of 0.16–0.05 units (µM glucose release/min/mg) at 50 °C and pH 12.0. TcEG1 activity from air-dried leaves was unchanged from that from green tissue, but when tissue was dried in a desiccant oven (46 °C), specific enzyme activity decreased by 60%. When transgenic biomass was “dropped-in” into an alkaline buffer (pH 12.0) and allowed to incubate at 50 °C, cellobiose release was increased up to 77% over non-transgenic biomass. Saccharification was increased in one transgenic event by 28%, which had a concurrent decrease in lignin content of 9%. Histological analysis revealed an increase in cell wall thickness with no change to cell area or perimeter. Transgenic plants produced more, albeit narrower, tillers with equivalent dry biomass as the control. Conclusions This work describes the first study in which an insect cellulase has been produced in transgenic plants; in this case, the dedicated bioenergy crop switchgrass. Switchgrass overexpressing the TcEG1 gene appeared to be morphologically similar to its non-transgenic control and produced equivalent dry biomass. Therefore, we propose TcEG1 transgenics could be bred with other transgenic germplasm (e.g., low-lignin lines) to yield new switchgrass with synergistically reduced recalcitrance to biofuel production. In addition, transgenes for other cell wall degrading enzymes may be stacked with TcEG1 in switchgrass to yield complementary cell wall digestion features and complete auto-hydrolysis
Emergence and maintenance of actionable genetic drivers at medulloblastoma relapse
BACKGROUND: 90% of tumors) and established genetic drivers (e.g. SHH/WNT/P53 mutations; 60% of rMB events) were maintained from diagnosis. Critically, acquired and maintained rMB events converged on targetable pathways which were significantly enriched at relapse (e.g. DNA damage-signaling) and specific events (e.g. 3p loss) predicted survival post-relapse. CONCLUSIONS: rMB is defined by the emergence of novel events and pathways, in concert with selective maintenance of established genetic drivers. Together, these define the actionable genetic landscape of rMB and provide a basis for improved clinical management and development of stratified therapeutics, across disease-course
Should Research Ethics Encourage the Production of Cost-Effective Interventions?
This project considers whether and how research ethics can contribute to the provision of cost-effective medical interventions. Clinical research ethics represents an underexplored context for the promotion of cost-effectiveness. In particular, although scholars have recently argued that research on less-expensive, less-effective interventions can be ethical, there has been little or no discussion of whether ethical considerations justify curtailing research on more expensive, more effective interventions. Yet considering cost-effectiveness at the research stage can help ensure that scarce resources such as tissue samples or limited subject popula- tions are employed where they do the most good; can support parallel efforts by providers and insurers to promote cost-effectiveness; and can ensure that research has social value and benefits subjects. I discuss and rebut potential objections to the consideration of cost-effectiveness in research, including the difficulty of predicting effectiveness and cost at the research stage, concerns about limitations in cost-effectiveness analysis, and worries about overly limiting researchers’ freedom. I then consider the advantages and disadvantages of having certain participants in the research enterprise, including IRBs, advisory committees, sponsors, investigators, and subjects, consider cost-effectiveness. The project concludes by qualifiedly endorsing the consideration of cost-effectiveness at the research stage. While incorporating cost-effectiveness considerations into the ethical evaluation of human subjects research will not on its own ensure that the health care system realizes cost-effectiveness goals, doing so nonetheless represents an important part of a broader effort to control rising medical costs
Advances in Global and Local Helioseismology: an Introductory Review
Helioseismology studies the structure and dynamics of the Sun's interior by
observing oscillations on the surface. These studies provide information about
the physical processes that control the evolution and magnetic activity of the
Sun. In recent years, helioseismology has made substantial progress towards the
understanding of the physics of solar oscillations and the physical processes
inside the Sun, thanks to observational, theoretical and modeling efforts. In
addition to the global seismology of the Sun based on measurements of global
oscillation modes, a new field of local helioseismology, which studies
oscillation travel times and local frequency shifts, has been developed. It is
capable of providing 3D images of the subsurface structures and flows. The
basic principles, recent advances and perspectives of global and local
helioseismology are reviewed in this article.Comment: 86 pages, 46 figures; "Pulsation of the Sun and Stars", Lecture Notes
in Physics, Vol. 832, Rozelot, Jean-Pierre; Neiner, Coralie (Eds.), 201
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