Would Nesotriatoma bruneri Usinger, 1944 be a valid species?

Alevi, Kaio C. C., Reis, Yago V., Guerra, Ana L., Imperador, Carlos H. L., Banho, Cecília A., Moreira, Felipe F. F., Azeredo-Oliveira, Maria T. V. (2016): Would Nesotriatoma bruneri Usinger, 1944 be a valid species? Zootaxa 4103 (4): 396-400, DOI: 10.11646/zootaxa.4103.4.

Study of nucleolar behavior during spermatogenesis in Martarega brasiliensis (Heteroptera, Notonectidae)

Submitted by sandra infurna ([email protected]) on 2016-05-13T18:01:25Z No. of bitstreams: 1 fff_moreira_etal_IOC_2015.pdf: 239117 bytes, checksum: c7f1f90c2207a5cda43541a3ad54f4ab (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-05-13T18:19:10Z (GMT) No. of bitstreams: 1 fff_moreira_etal_IOC_2015.pdf: 239117 bytes, checksum: c7f1f90c2207a5cda43541a3ad54f4ab (MD5)Made available in DSpace on 2016-05-13T18:19:10Z (GMT). No. of bitstreams: 1 fff_moreira_etal_IOC_2015.pdf: 239117 bytes, checksum: c7f1f90c2207a5cda43541a3ad54f4ab (MD5) Previous issue date: 2015Universidade Estadual Paulista “Júlio de Mesquita Filho". Instituto de Biociências, Letras e Ciências Exatas. Departamento de Biologia. Laboratório de Citogenética e Molecular de Insetos. São José do Rio Preto, SP, Brasil.Universidade Estadual Paulista “Júlio de Mesquita Filho”. Instituto de Biociências, Letras e Ciências Exatas. Departamento de Biologia. Laboratório de Biologia Celular. São José do Rio Preto, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Departamento de Zoologia. Laboratório de Entomologia. Rio de Janeiro, RJ, Brasil.Universidade Estadual Paulista “Júlio de Mesquita Filho". Instituto de Biociências, Letras e Ciências Exatas. Departamento de Biologia. Laboratório de Citogenética e Molecular de Insetos. São José do Rio Preto, SP, Brasil.Universidade Estadual Paulista “Júlio de Mesquita Filho". Instituto de Biociências, Letras e Ciências Exatas. Departamento de Biologia. Laboratório de Citogenética e Molecular de Insetos. São José do Rio Preto, SP, Brasil.Universidade Estadual Paulista “Júlio de Mesquita Filho". Instituto de Biociências, Letras e Ciências Exatas. Departamento de Biologia. Laboratório de Citogenética e Molecular de Insetos. São José do Rio Preto, SP, Brasil.Universidade Estadual Paulista “Júlio de Mesquita Filho". Instituto de Biociências, Letras e Ciências Exatas. Departamento de Biologia. Laboratório de Citogenética e Molecular de Insetos. São José do Rio Preto, SP, Brasil.Few cytogenetic studies have been undertaken using aquatic heteropterans and the nucleolar behavior of these insects has been described in only four species, Limnogonus aduncus, Brachymetra albinerva, Halobatopsis platensis, and Cylindrostethus palmaris. The nucleolus is a cellular structure related to biosynthetic activity and it exhibits a peculiar behavior in the heteropterans of the Triatominae subfamily; it persists during all stages of meiosis. Thus, this study aims to analyze spermatogenesis in Martarega brasiliensis, with an emphasis on nucleolar behavior. Twenty M. brasiliensis adult males were used and collected from the Municipal reservoir in the city of São José do Rio Preto, São Paulo, Brazil. The species were fixed in methanol:acetic acid (3:1), then dissected, and the testicles were extracted, torn apart, and impregnated with silver ions. During prophase, the nuclei of M. brasiliensis were composed of the nucleolus and nucleolar corpuscles, which varied in number from one to four, emphasizing that this insect has great synthetic activity during meiosis. The analysis of cells in metaphase I showed that M. brasiliensis presents a nucleolar organizing region in at least one autosome. Furthermore, the phenomenon of nucleolar persistence was not observed. All spermatids presented nucleolar markings that varied in number and position according to the stage of elongation. Moreover, it was also possible to highlight the presence of a vesicle in spermatids. Thus, this paper describes the nucleolar behavior of M. brasiliensis and highlights important characteristics during spermatogenesis, thus, increasing the knowledge about the biology of these aquatic heteropterans

Clinical Characterization of Respiratory Syncytial Virus Infection in Adults: A Neglected Disease?

Lower respiratory tract infections (LRIs) are a significant cause of disability-adjusted life-years (DALYs) across all age groups, especially in children under 9 years of age, and adults over 75. The main causative agents are viruses, such as influenza and respiratory syncytial virus (RSV). Viral LRIs in adults have historically received less attention. This study investigated the incidence of RSV and influenza in adult patients admitted to a referral hospital, as well as the clinical profile of these infections. Molecular testing was conducted on nasopharyngeal samples taken from a respiratory surveillance cohort comprising adult (15–59 years) and elderly (60+ years) hospitalized patients who tested negative for SARS-CoV-2, to determine the prevalence for influenza and RSV. Influenza was found to be less frequent among the elderly. The main symptoms of RSV infections were cough, fever, dyspnea, malaise, and respiratory distress, while headache, nasal congestion, a sore throat, and myalgia were most frequent in influenza. Elderly patients with RSV were not found to have more severe illness than adults under age 60, underscoring the importance of providing the same care to adults with this viral infection

Genomic epidemiology reveals how restriction measures shaped the SARS-CoV-2 epidemic in Brazil

Abstract Brazil has experienced some of the highest numbers of COVID-19 infections and deaths globally and made Latin America a pandemic epicenter from May 2021. Although SARS-CoV-2 established sustained transmission in Brazil early in the pandemic, important gaps remain in our understanding of local virus transmission dynamics. Here, we describe the genomic epidemiology of SARS-CoV-2 using near-full genomes sampled from 27 Brazilian states and an adjacent country - Paraguay. We show that the early stage of the pandemic in Brazil was characterised by the co-circulation of multiple viral lineages, linked to multiple importations predominantly from Europe, and subsequently characterized by large local transmission clusters. As the epidemic progressed, the absence of effective restriction measures led to the local emergence and international spread of Variants of Concern (VOC) and under monitoring (VUM), including the Gamma (P.1) and Zeta (P.2) variants. In addition, we provide a preliminary genomic overview of the epidemic in Paraguay, showing evidence of importation from Brazil. These data reinforce the need for the implementation of widespread genomic surveillance in South America as a toolkit for pandemic monitoring and providing a means to follow the real-time spread of emerging SARS-CoV-2 variants with possible implications for public health and immunization strategies