The role of phosphorylation and dephosphorylation of shell matrix proteins in shell formation : an in vivo and in vitro study

Protein phosphorylation is a fundamental mechanism regulating many aspects of cellular processes. Shell matrix proteins (SMPs) control crystal nucleation, polymorphism, morphology, and organization of calcium carbonate crystallites during shell formation. SMPs phosphorylation is suggested to be important in shell formation but the mechanism is largely unknown. Here, to investigate the mechanism of phosphorylation of SMPs in biomineralization, we performed in vivo and in vitro experiment. By injection of antibody against the anti-phosphoserine/threonine /tyrosine into the extrapallial fluid of the pearl oyster Pinctada fucata, phosphorylation of matrix proteins were significantly reduced after 6 days. Newly formed prismatic layers and nacre tablet were found to grow abnormally with reduced crystallinity and possibly changed crystal orientation shown by Raman spectroscopy. In addition, regeneration of shells is also inhibited in vivo. Then, protein phosphatase was used to dephosphorylate SMPs extracted from the shells. After dephosphorylation, the ability of SMPs to inhibiting calcium carbonate formation have been reduced. Surprisingly, the ability of SMPs to modulate crystal morphology have been largely compromised although phosphorylation extent remained to be at least half of the control. Furthermore, dephosphorylation of SMPs changed the distribution of protein occlusions and decreased the amount of protein occlusions inside crystals shown by confocal imaging, indicating interaction between phosphorylated SMPs and crystals. Taken together, this study provides insight into the mechanism of phosphorylation of SMPs during shell formation

Microwave photoresistance of a high-mobility two-dimensional electron gas in a triangular antidot lattice

The microwave (MW) photoresistance has been measured on a high-mobility two-dimensional electron gas patterned with a shallow triangular antidot lattice, where both the MW-induced resistance oscillations (MIRO) and magnetoplasmon (MP) resonance are observed superposing on sharp commensurate geometrical resonance (GR). Analysis shows that the MIRO, MP, and GR are decoupled from each other in these experiments.Comment: 5 pages, 4 figures, paper accepted by PR

The hydrostatic equilibrium and Tsallis equilibrium for self-gravitating systems

Self-gravitating systems are generally thought to behavior non-extensively due to the long-range nature of gravitational forces. We obtain a relation between the nonextensive parameter q of Tsallis statistics, the temperature gradient and the gravitational potential based on the equation of hydrostatic equilibrium of self-gravitating systems. It is suggested that the nonextensive parameter in Tsallis statistics has a clear physical meaning with regard to the non-isothermal nature of the systems with long-range interactions and Tsallis equilibrium distribution for the self-gravitating systems describes the property of hydrostatic equilibrium of the systems.Comment: 7 pages, 9 Reference

Effective Mass of the Four Flux Composite Fermion at ν=1/4\nu = 1/4

We have measured the effective mass ($m^*$) of the four flux composite fermion at Landau level filling factor $\nu = 1/4$ ($^4$CF), using the activation energy gaps at the fractional quantum Hall effect (FQHE) states $\nu$ = 2/7, 3/11, and 4/15 and the temperature dependence of the Shubnikov-de Haas (SdH) oscillations around $\nu = 1/4$. We find that the energy gaps show a linear dependence on the effective magnetic field $B_{eff}$ ($\equiv B-B_{\nu=1/4}$), and from this linear dependence we obtain $m^* = 1.0 m_e$ and a disorder broadening $\Gamma \sim$ 1 K for a sample of density $n = 0.87 \times 10^{11}$ /cm$^2$. The $m^*$ deduced from the temperature dependence of the SdH effect shows large differences for $\nu > 1/4$ and $\nu < 1/4$. For $\nu > 1/4$, $m^* \sim 1.0 m_e$. It scales as $\sqrt{B_{\nu}}$ with the mass derived from the data around $\nu =1/2$ and shows an increase in $m^*$ as $\nu \to 1/4$, resembling the findings around $\nu =1/2$. For $\nu < 1/4$, $m^*$ increases rapidly with increasing $B_{eff}$ and can be described by $m^*/m_e = -3.3 + 5.7 \times B_{eff}$. This anomalous dependence on $B_{eff}$ is precursory to the formation of the insulating phase at still lower filling.Comment: 5 pages, 3 figure

Degenerate states of narrow semiconductor rings in the presence of spin orbit coupling: Role of time-reversal and large gauge transformations

The electron Hamiltonian of narrow semiconductor rings with the Rashba and Dresselhaus spin orbit terms is invariant under time-reversal operation followed by a large gauge transformation. We find that all the eigenstates are doubly degenerate when integer or half-integer quantum fluxes thread the quantum ring. The wavefunctions of a degenerate pair are related to each other by the symmetry operation. These results are valid even in the presence of a disorder potential. When the Zeeman term is present only some of these degenerate levels anticross

Semiclassical Green Function in Mixed Spaces

A explicit formula on semiclassical Green functions in mixed position and momentum spaces is given, which is based on Maslov's multi-dimensional semiclassical theory. The general formula includes both coordinate and momentum representations of Green functions as two special cases of the form.Comment: 8 pages, typeset by Scientific Wor

Power Corrections in Charmless B Decays

In this paper, we focus on the role of power corrections in QCD factorization(QCDF) method in charmless two-body nonleptonic $B$ meson decays. We use the ratio of the branching fraction of $B^+ \to \pi^+ K^{\ast 0}$ to that of $B^0 \to \pi^- \rho^+$, for which the theoretical uncertainties are greatly reduced, to show clearly that the power corrections in charmless B decays are probably large. With other similar ratios considered, for example, for the $B^0 \to K^- \rho^+$ decay, it is very likely that, among various sources of power corrections, annihilation topology plays an indispensable role at least for penguin dominated $\rm PV$ channels. We also consider some selective ratios of direct CP asymmetries. Among these, we find that, if power corrections other than the chirally enhanced power corrections and annihilation topology were negligible, QCDF would predict the direct CP asymmetry of $B \to \pi^+ \pi^-$ to be about 3 times larger than that of $B \to \pi^\pm K^\mp$, with opposite sign. Experimentally any significant deviation from this prediction would suggest either new physics or possibly the importance of long-distance rescattering effects.Comment: references and note added, to appear in Phys. Rev.

Novel insights into transfer processes in the reaction 16O+208Pb at sub-barrier energies

The collision of the doubly-magic nuclei $^{16}$O+$^{208}$Pb is a benchmark in nuclear reaction studies. Our new measurements of back-scattered projectile-like fragments at sub-barrier energies show show that transfer of 2 protons ($2p$) is much more probable than $\alpha$-particle transfer. $2p$ transfer probabilities are strongly enhanced compared to expectations for the sequential transfer of two uncorrelated protons; at energies around the fusion barrier absolute probabilities for two proton transfer are similar to those for one proton transfer. This strong enhancement indicates strong $2p$ pairing correlations in $^{16}$O, and suggests evidence for the occurrence of a nuclear supercurrent of two-proton Cooper pairs in this reaction, already at energies well below the fusion barrier.Comment: 5 pages, 3 figure

Enhancement of the ν=5/2\nu = 5/2 Fractional Quantum Hall State in a Small In-Plane Magnetic Field

Using a 50-nm width, ultra-clean GaAs/AlGaAs quantum well, we have studied the Landau level filling factor $\nu = 5/2$ fractional quantum Hall effect in a perpendicular magnetic field $B \sim$ 1.7 T and determined its dependence on tilted magnetic fields. Contrary to all previous results, the 5/2 resistance minimum and the Hall plateau are found to strengthen continuously under an increasing tilt angle $0 < \theta < 25^\circ$ (corresponding to an in-plane magnetic field 0 $<$ $B_\parallel$ $< 0.8$ T). In the same range of $\theta$ the activation gaps of both the 7/3 and the 8/3 states are found to increase with tilt. The 5/2 state transforms into a compressible Fermi liquid upon tilt angle $\theta > 60^\circ$, and the composite fermion series [2+$p/(2p\pm1)$], $p =$ 1, 2 can be identified. Based on our results, we discuss the relevance of a Skyrmion spin texture at $\nu = 5/2$ associated with small Zeeman energy in wide quantum wells, as proposed by W$\acute{\text o}$js $et$ $al$., Phys. Rev. Lett. 104, 086801 (2010).Comment: 5+ pages, 3 figures, accepted for by Phy. Rev. Let