1,498 research outputs found
Negative Electron-electron Drag Between Narrow Quantum Hall Channels
Momentum transfer due to Coulomb interaction between two parallel,
two-dimensional, narrow, and spatially separated layers, when a current
I_{drive} is driven through one layer, is studied in the presence of a
perpendicular magnetic field B. The current induced in the drag layer,
I_{drag}, is evaluated self-consistently with I_{drive} as a parameter.
I_{drag} can be positive or negative depending on the value of the filling
factor \nu of the highest occupied bulk Landau level (LL). For a fully occupied
LL, I_{drag} is negative, i.e., it flows opposite to I_{drive}, whereas it is
positive for a half-filled LL. When the circuit is opened in the drag layer, a
voltage \Delta V_{drag} develops in it; it is negative for a half-filled LL and
positive for a fully occupied LL. This positive \Delta V_{drag}, expressing a
negative Coulomb drag, results from energetically favored near-edge inter-LL
transitions that occur when the highest occupied bulk LL and the LL just above
it become degenerate.Comment: Text file in Latex/Revtex/preprint format, 7 separate PS figures,
Physical Review B, in pres
Frictional drag between non-equilibrium charged gases
The frictional drag force between separated but coupled two-dimensional
electron gases of different temperatures is studied using the non-equilibrium
Green function method based on the separation of center-of-mass and relative
dynamics of electrons. As the mechanisms of producing the frictional force we
include the direct Coulomb interaction, the interaction mediated via virtual
and real TA and LA phonons, optic phonons, plasmons, and TA and LA
phonon-electron collective modes. We found that, when the distance between the
two electron gases is large, and at intermediate temperature where plasmons and
collective modes play the most important role in the frictional drag, the
possibility of having a temperature difference between two subsystems modifies
greatly the transresistivity.Comment: 8figure
Ribosomal Proteins RPS11 and RPS20, Two Stress-Response Markers of Glioblastoma Stem Cells, Are Novel Predictors of Poor Prognosis in Glioblastoma Patients.
Glioblastoma stem cells (GSC) co-exhibiting a tumor-initiating capacity and a radio-chemoresistant phenotype, are a compelling cell model for explaining tumor recurrence. We have previously characterized patient-derived, treatment-resistant GSC clones (TRGC) that survived radiochemotherapy. Compared to glucose-dependent, treatment-sensitive GSC clones (TSGC), TRGC exhibited reduced glucose dependence that favor the fatty acid oxidation pathway as their energy source. Using comparative genome-wide transcriptome analysis, a series of defense signatures associated with TRGC survival were identified and verified by siRNA-based gene knockdown experiments that led to loss of cell integrity. In this study, we investigate the prognostic value of defense signatures in glioblastoma (GBM) patients using gene expression analysis with Probeset Analyzer (131 GBM) and The Cancer Genome Atlas (TCGA) data, and protein expression with a tissue microarray (50 GBM), yielding the first TRGC-derived prognostic biomarkers for GBM patients. Ribosomal protein S11 (RPS11), RPS20, individually and together, consistently predicted poor survival of newly diagnosed primary GBM tumors when overexpressed at the RNA or protein level [RPS11: Hazard Ratio (HR) = 11.5, p<0.001; RPS20: HR = 4.5, p = 0.03; RPS11+RPS20: HR = 17.99, p = 0.001]. The prognostic significance of RPS11 and RPS20 was further supported by whole tissue section RPS11 immunostaining (27 GBM; HR = 4.05, p = 0.01) and TCGA gene expression data (578 primary GBM; RPS11: HR = 1.19, p = 0.06; RPS20: HR = 1.25, p = 0.02; RPS11+RPS20: HR = 1.43, p = 0.01). Moreover, tumors that exhibited unmethylated O-6-methylguanine-DNA methyltransferase (MGMT) or wild-type isocitrate dehydrogenase 1 (IDH1) were associated with higher RPS11 expression levels [corr (IDH1, RPS11) = 0.64, p = 0.03); [corr (MGMT, RPS11) = 0.52, p = 0.04]. These data indicate that increased expression of RPS11 and RPS20 predicts shorter patient survival. The study also suggests that TRGC are clinically relevant cells that represent resistant tumorigenic clones from patient tumors and that their properties, at least in part, are reflected in poor-prognosis GBM. The screening of TRGC signatures may represent a novel alternative strategy for identifying new prognostic biomarkers
Bone morphogenetic protein 7 sensitizes O6-methylguanine methyltransferase expressing-glioblastoma stem cells to clinically relevant dose of temozolomide.
BackgroundTemozolomide (TMZ) is an oral DNA-alkylating agent used for treating patients with glioblastoma. However, therapeutic benefits of TMZ can be compromised by the expression of O6-methylguanine methyltransferase (MGMT) in tumor tissue. Here we used MGMT-expressing glioblastoma stem cells (GSC) lines as a model for investigating the molecular mechanism underlying TMZ resistance, while aiming to explore a new treatment strategy designed to possibly overcome resistance to the clinically relevant dose of TMZ (35 μM).MethodsMGMT-expressing GSC cultures are resistant to TMZ, and IC50 (half maximal inhibitory concentration) is estimated at around 500 μM. Clonogenic GSC surviving 500 μM TMZ (GSC-500 μM TMZ), were isolated. Molecular signatures were identified via comparative analysis of expression microarray against parental GSC (GSC-parental). The recombinant protein of top downregulated signature was used as a single agent or in combination with TMZ, for evaluating therapeutic effects of treatment of GSC.ResultsThe molecular signatures characterized an activation of protective stress responses in GSC-500 μM TMZ, mainly including biotransformation/detoxification of xenobiotics, blocked endoplasmic reticulum stress-mediated apoptosis, epithelial-to-mesenchymal transition (EMT), and inhibited growth/differentiation. Bone morphogenetic protein 7 (BMP7) was identified as the top down-regulated gene in GSC-500 μM TMZ. Although augmenting BMP7 signaling in GSC by exogenous BMP7 treatment did not effectively stop GSC growth, it markedly sensitized both GSC-500 μM TMZ and GSC-parental to 35 μM TMZ treatment, leading to loss of self-renewal and migration capacity. BMP7 treatment induced senescence of GSC cultures and suppressed mRNA expression of CD133, MGMT, and ATP-binding cassette drug efflux transporters (ABCB1, ABCG2), as well as reconfigured transcriptional profiles in GSC by downregulating genes associated with EMT/migration/invasion, stemness, inflammation/immune response, and cell proliferation/tumorigenesis. BMP7 treatment significantly prolonged survival time of animals intracranially inoculated with GSC when compared to those untreated or treated with TMZ alone (p = 0.0017), whereas combination of two agents further extended animal survival compared to BMP7 alone (p = 0.0489).ConclusionsThese data support the view that reduced endogenous BMP7 expression/signaling in GSC may contribute to maintained stemness, EMT, and chemoresistant phenotype, suggesting that BMP7 treatment may provide a novel strategy in combination with TMZ for an effective treatment of glioblastoma exhibiting unmethylated MGMT
Z-dependent Barriers in Multifragmentation from Poissonian Reducibility and Thermal Scaling
We explore the natural limit of binomial reducibility in nuclear
multifragmentation by constructing excitation functions for intermediate mass
fragments (IMF) of a given element Z. The resulting multiplicity distributions
for each window of transverse energy are Poissonian. Thermal scaling is
observed in the linear Arrhenius plots made from the average multiplicity of
each element. ``Emission barriers'' are extracted from the slopes of the
Arrhenius plots and their possible origin is discussed.Comment: 15 pages including 4 .ps figures. Submitted to Phys. Rev. Letters.
Also available at http://csa5.lbl.gov/moretto
Phonon mediated drag in double layer two dimensional electron systems
Experiments studying phonon mediated drag in the double layer two dimensional
electron gas system are reported. Detailed measurements of the dependence of
drag on temperature, layer spacing, density ratio, and matched density are
discussed. Comparisons are made to theoretical results [M. C. Bonsager et al.,
Phys. Rev. B 57, 7085 (1998)] which propose the existence of a new coupled
electron-phonon collective mode. The layer spacing and density dependence at
matched densities for samples with layer spacings below 2600 A do not support
the existence of this mode, showing behavior expected for independent electron
and phonon systems. The magnitude of the drag, however, suggests the alternate
limit; one in which electrons and phonons are strongly coupled. The results for
still larger layer spacing show significant discrepancies with the behavior
expected for either limit.Comment: 9 pages, 9 figures, Late
A statistical interpretation of the correlation between intermediate mass fragment multiplicity and transverse energy
Multifragment emission following Xe+Au collisions at 30, 40, 50 and 60 AMeV
has been studied with multidetector systems covering nearly 4-pi in solid
angle. The correlations of both the intermediate mass fragment and light
charged particle multiplicities with the transverse energy are explored. A
comparison is made with results from a similar system, Xe+Bi at 28 AMeV. The
experimental trends are compared to statistical model predictions.Comment: 7 pages, submitted to Phys. Rev.
Magneto-Coulomb drag: interplay of electron--electron interactions and Landau quantization
We use the Kubo formalism to calculate the transresistivity for
carriers in coupled quantum wells in a large perpendicular magnetic field .
We find that is enhanced by approximately 50--100 times over that
of the B=0 case in the interplateau regions of the integer quantum Hall effect.
The presence of both electron--electron interactions and Landau quantization
results in (i) a twin-peaked structure of in the inter-plateau
regions at low temperatures, and, (ii) for the chemical potential at the center
of a Landau level band, a peaked temperature dependence of .Comment: 4 pages, RevTeX, 4 PS figures in text using eps
The Quantum Hall Effect in Drag: Inter-layer Friction in Strong Magnetic Fields
We study the Coulomb drag between two spatially separated electron systems in
a strong magnetic field, one of which exhibits the quantum Hall effect. At a
fixed temperature, the drag mimics the behavior of in the quantum
Hall system, in that it is sharply peaked near the transitions between
neighboring plateaux. We assess the impact of critical fluctuations near the
transitions, and find that the low temperature behavior of the drag measures an
exponent that characterizes anomalous low frequency dissipation; the
latter is believed to be present following the work of Chalker.Comment: 13 pages, Revtex 2.0, 1 figure upon request, P-93-11-09
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