Expression a La Bimode

The recently published article by Wang, and commented on by Ertel in this journal, describes development of a method, named the Bimodality Index, to objectively identify and rank meaningful and reliable bimodal patterns from large-scale gene expression datasets. This is an important step because genes with a bimodal distribution may be prime candidates to develop as molecular diagnostic tests for distinguishing clinically important groups. In their introduction, they make the point that a bimodal expression pattern may be observed when two distinct subgroups of samples are measured as one group, with each mode representing the mean expression of a gene in one of the sub-groups. Recently, it was reported that bimodal gene expression may represent another important phenomenon. Specifically, two clinically important groups may be distinguished on the basis of a gene being unimodal in one group and bimodal in the other group. For example, certain key antioxidant, DNA repair, and transcription factor genes each display a unimodal pattern in non-lung cancer subjects but a bimodal pattern in lung cancer subjects. Importantly, when two groups are distinguished on the basis of unimodal vs bimodal distribution of an analyte (in this case gene expression value), biomarker development requires use of two cut-points, one on either side of the unimodal distribution. It is timely then that an improved method for separating groups on the basis of two cut-points was described recently. Thus, the analytical method to discover genes with bimodal expression distribution described by Wang and recent statistical methods that enable separation of groups on the basis of two-cut points are likely to accelerate both diagnostic test discovery, as well as mechanistic understanding of disease and disease risk. It is important to recognize that transcript abundance methods vary in their linear dynamic range, and that the ability to identify bimodal distribution of genes will depend to a significant degree on the linear dynamic range of the method used

The Discoverability of Award-Winning Undergraduate Research in History: Implications for Academic Libraries

Making scholarly information visible to web search engines is an ongoing challenge, and undergraduate research is no exception. Using a sample of award-winning undergraduate history papers and journals, the authors searched Google, Google Scholar, Microsoft Academic, and the authors’ institutional repository to gauge the difficulty of locating these works. Given that many of these works were not easily found, results suggest that libraries and their institutions could be doing more to increase the discoverability of undergraduate research. Based on the success stories observed in this study, we offer strategies to libraries and librarians for increasing the visibility of undergraduate student research

Soil availability, plant uptake and soil to plant transfer of 99Tc - A review

The fission yield of 99Tc from 239Pu and 235U is similar to that of 137Cs or 90Sr and it is therefore an important component of nuclear weapons fall-out, nuclear waste and releases from nuclear facilities. There is particular current interest in 99Tc transfer from soil to plants for: (a) environmental impact assessments for terrestrial nuclear waste repositories, and (b) assessments of the potential for phytoextraction of radionuclides from contaminated effluent and soil. Vascular plants have a high 99Tc uptake capacity, a strong tendency to transport it to shoot material and accumulate it in vegetative rather than reproductive structures. The mechanisms that control 99Tc entry to plants have not been identified and there has been little discussion of the potential for phytoextraction of 99Tc contaminated effluents or soil. Here we review soil availability, plant uptake mechanisms and soil to plant transfer of 99Tc in the light of recent advances in soil science, plant molecular biology and phytoextraction technologies. We conclude that 99Tc might not be highly available in the long term from up to 50% of soils worldwide, and that no single mechanism that might be easily targeted by recombinant DNA technologies controls 99Tc uptake by plants. Overall, we suggest that Tc might be less available in terrestrial ecosystems than is often assumed but that nevertheless the potential of phytoextraction as a decontamination strategy is probably greater for 99Tc than for any other nuclide of radioecological interest. © 2002 Elsevier Science Ltd. All rights reserved

First-Year Movements by Juvenile Mexican Spotted Owls in the Canyonlands of Utah

We studied first-year movements of Mexican Spotted Owls (Strix occidentalis lucida) during natal dispersal in canyonlands of southern Utah. Thirty-one juvenile Mexican Spotted Owls were captured and radiotracked during 1992-95 to examine behavior and conduct experiments related to the onset of natal dispersal. Juvenile Spotted Owls dispersed from their nest areas during September to October each year, with 85% leaving in September. The onset of movements was sudden and juveniles dispersed in varied directions. The median distance from nest area to last observed location was 25.7 km (range = 1.7-92.3 km). Three of 26 juveniles tracked (11%) were alive after one year, although none were observed with mates. We conducted a feeding experiment, using Mongolian gerbils (Meriones unguicuculatus), to test the influence of increased food supply on dispersal onset. The mean dispersal date of five owls that received supplemental food Julian day no. 255 +/- 2.6 SD) was significantly different than a control group (day no. 273 +/- 12.3)

A flight and wind tunnel investigation of the effect of angle-of-attack rate on maximum lift coefficient

Wind tunnel tests to determine effect of angle of attack on maximum lift at stall for half-wing models, and single engine, jet propelled aircraf

Quality Control Methods for Optimal BCR-ABL1 Clinical Testing in Human Whole Blood Samples

Reliable breakpoint cluster region (BCR)–Abelson (ABL) 1 measurement is essential for optimal management of chronic myelogenous leukemia. There is a need to optimize quality control, sensitivity, and reliability of methods used to measure a major molecular response and/or treatment failure. The effects of room temperature storage time, different primers, and RNA input in the reverse transcription (RT) reaction on BCR-ABL1 and β-glucuronidase (GUSB) cDNA yield were assessed in whole blood samples mixed with K562 cells. BCR-ABL1 was measured relative to GUSB to control for sample loading, and each gene was measured relative to known numbers of respective internal standard molecules to control for variation in quality and quantity of reagents, thermal cycler conditions, and presence of PCR inhibitors. Clinical sample and reference material measurements with this test were concordant with results reported by other laboratories. BCR-ABL1 per 103 GUSB values were significantly reduced (P = 0.004) after 48-hour storage. Gene-specific primers yielded more BCR-ABL1 cDNA than random hexamers at each RNA input. In addition, increasing RNA inhibited the RT reaction with random hexamers but not with gene-specific primers. Consequently, the yield of BCR-ABL1 was higher with gene-specific RT primers at all RNA inputs tested, increasing to as much as 158-fold. We conclude that optimal measurement of BCR-ABL1 per 103 GUSB in whole blood is obtained when gene-specific primers are used in RT and samples are analyzed within 24 hours after blood collection

Psychosocial Impacts of the COVID-19 Pandemic: The Role of Men’s Gender-Related Attitudes, Employment and Housework, and Demographic Characteristics

Background and objective: Globally, men are at greater risk of mortality and serious physical consequences from COVID-19 infection than women, but are less impacted by the pandemic’s impact on labor force participation and increased childcare responsibilities. Outside of gender identity, however, it is unclear whether men’s beliefs about gender may be related to the kinds of COVID-19-related impacts they report. This study sought to describe the employment, income, and household responsibility-related impacts of the pandemic on a sample of young men in the U.S. and to examine relationships between the men’s gender ideologies and attitudes toward gender equity with self-reported stress impacts of the pandemic. Methods: The data are from an online survey of 481 young men from across the U.S. Measures included scales assessing masculinity ideology, modern sexism, support for traditional divisions of labor by gender, and attitudes toward gender equity. New items developed for this study assessed COVID-19-related changes in employment, household responsibilities, and childcare duties as well as levels of stress. Hierarchical regression examined the relative roles of demographic characteristics, changes in employment and household work, and gender-related attitudes on COVID-related stress. Results: Descriptive findings showed that under 50% of the men in the sample experienced negative COVID-related impacts on employment, but that a majority of the men reported at least some COVID-related stress. Results of the hierarchical regression suggest that higher levels of stress were predicted by having a minoritized sexual identity, less religiosity, experiencing employment or household responsibility-related changes, and not endorsing modern sexism or a traditional, gendered division of labor. Conclusions: Experiencing COVID-19-related stress was normative in this sample of young men. However, endorsing traditional notions of a gendered division of labor was slightly protective against higher levels of COVID-related stress. These findings add to existing evidence that gender analysis must be a central component of ongoing COVID-related policy and programming development

Trade Secret Law and Information Systems: Can Your Students Keep a Secret?

The impact of intellectual property (IP) law on information systems (IS) professionals in business cannot be overstated. The IS 2010 model curriculum guidelines for undergraduate IS programs stress the importance of information security and knowledge about IP. While copyright and patents are the most well-known types of IP, another, trade secrets, which involve confidential information generated by business to secure financial success, poses a unique challenge partly because IS professionals are often less familiar with trade secrets as a form of IP. Just as important is the crucial role IS plays in actually creating trade secrets. Information must not only be vital and proprietary but also its secrecy must be actively protected and maintained against data security challenges, including unethical behavior by disgruntled employees, corporate espionage, and inadvertent disclosure. Failure to do so results in a determination that information is not legally a protected trade secret. Unlike copyrights and patents, information cannot publically be designated as a trade secret prior to a challenge. Instead, organizations must prove the information is actually a trade secret. Critical to this proof are processes and internal systems businesses use to maintain information secrecy, which determine whether legal remedies exist if the trade secret is wrongfully divulged. This paper discusses trade secret law, methods used to secure trade secrets, and the role of IS in supporting and/or developing those methods. A class exercise provides IS students with insights into trade secret law and acceptable, ethical conduct of IS professionals who protect trade secrets