Silencing of Vlaro2 for chorismate synthase revealed that the phytopathogen Verticillium longisporum induces the cross-pathway control in the xylem

The first leaky auxotrophic mutant for aromatic amino acids of the near-diploid fungal plant pathogen Verticillium longisporum (VL) has been generated. VL enters its host Brassica napus through the roots and colonizes the xylem vessels. The xylem contains little nutrients including low concentrations of amino acids. We isolated the gene Vlaro2 encoding chorismate synthase by complementation of the corresponding yeast mutant strain. Chorismate synthase produces the first branch point intermediate of aromatic amino acid biosynthesis. A novel RNA-mediated gene silencing method reduced gene expression of both isogenes by 80% and resulted in a bradytrophic mutant, which is a leaky auxotroph due to impaired expression of chorismate synthase. In contrast to the wild type, silencing resulted in increased expression of the cross-pathway regulatory gene VlcpcA (similar to cpcA/GCN4) during saprotrophic life. The mutant fungus is still able to infect the host plant B. napus and the model Arabidopsis thaliana with reduced efficiency. VlcpcA expression is increased in planta in the mutant and the wild-type fungus. We assume that xylem colonization requires induction of the cross-pathway control, presumably because the fungus has to overcome imbalanced amino acid supply in the xylem

Routing in Post-Disaster Scenarios

Current networks should provide disaster-resilience by coping with the possible failures and misbehaviours caused by massive natural or man-made disasters. This is necessary to keep a suitable level of Quality of Service after a disaster and to support the possible evacuation, rescue, assessment, and rescue operations within the affected area. Multiple possible methods and solutions can be put in place in a proactive and/or reactive manner to offer the required resilience degree. Among them, a proper routing algorithm can contribute to circumventing network elements damaged by the disaster or applying for spatial/temporal redundancy to guarantee effective communications. This chapter aims at presenting the main routing solutions to offer disaster-resilience communications, along with some related methods

Automated valet parking and charging for e-mobility

Automated valet parking services provide great potential to increase the attractiveness of electric vehicles by mitigating their two main current deficiencies: reduced driving ranges and prolonged refueling times. The European research project V-Charge aims at providing this service on designated parking lots using close-to-market sensors only. For this purpose the project developed a prototype capable of performing fully automated navigation in mixed traffic on designated parking lots and GPS-denied parking garages with cameras and ultrasonic sensors only. This paper summarizes the work of the project, comprising advances in network communication and parking space scheduling, multi-camera calibration, semantic mapping concepts, visual localization and motion planning. The project pushed visual localization, environment perception and automated parking to centimetre precision. The developed infrastructure-based camera calibration and semi-supervised semantic mapping concepts greatly reduce maintenance efforts. Results are presented from extensive month-long field tests