EFFECT OF CE3+ METAL IONS ON THE ANTIBACTERIAL AND ANTICANCER ACTIVITY OF ZINC OXIDE NANOPARTICLES PREPARED BY COPRECIPITATION METHOD

ABSTRACTObjective: This study was undertaken to know about the antibacterial and anticancer activity of synthesized zinc oxide (ZnO) nanoparticles (NPs).Methods: The ZnO NPs and different concentration of Ce3+ (0.05M, 0.10M, and 0.15M)-doped ZnO NPs were synthesized by coprecipitation method.The synthesized nanoparticles were analyzed by X-ray diffraction (XRD) and HRSEM. The antibacterial studies were performed against a set ofbacterial strains as Gram-positive bacteria (Streptococcus aureus and Streptococcus pneumonia) and Gram-negative (Escherichia coli, Pseudomonasaeruginosa, Proteus vulgaris, Klebsiella pneumonia, and Shigella dysenteriae) bacteria. The cytotoxic effect of ZnO and Ce-doped ZnO was analyzed incultured (A549) human lung cancer cell line.Result: The XRD studies showed the wurtzite structure of nanoparticles. HRSEM analysis showed the spherical shape of ZnO and Ce-doped ZnO. TheZn0.85Ce0.15O NPs possessed more antibacterial effect as compared to the other ZnO and Ce-doped ZnO NPs. The Zn0.90Ce0.10O NPs created the highestcytotoxicity activity. With respect to cell death, as low a concentration of 68±0.05 μg/ml of Zn0.90Ce0.10O NPs was good enough to cause loss of viabilityof 50% of the cell as compared to ZnO and Zn1-xCexO (x=0.05 and 0.15) NPs.Conclusion: Results from this work concluded that Zn0.85Ce0.15O and Zn0.90Ce0.10O NPs possess antibacterial and anticancer activity, respectively.Keywords: Zinc oxide nanoparticles, Coprecipitation method, Antibacterial activity and anticancer activity, Human lung cancer cell line

Purification and characterization of membrane-bound <em>Borassus flabellifer</em> L. thermostable peroxidase

273-279Generating wealth out of waste is a goal in solid waste management. Borassus flabellifer shells with stone part are discarded as waste after removing its nutritious fruit. An enzyme extracted from the discarded stone part has potential application in molecular diagnosis and it is a wealth. The waste stone part bound Borassus flabellifer peroxidase was purified by salting-out, salting-in and DEAE-Cellulose anion exchange chromatographic technique to apparent homogeneity. Relative molecular weight under denaturating condition was around 40 kDa. The preparation had single isoenzyme as evidenced under nondenaturating condition. It was a glyco- and hemoprotein. It retained 100% activity for 120 h at 70°C. pH optima with benzidine, o-dianisidine, guaiacol and tetramethylbenzidine was around 5.0. Kinetic studies showed it had a higher affinity towards tetramethyl benzidine than other three substrates. The thermodynamic parameter of the enzyme with benzidine, o-dianisidine, guaiacol and tetramethylbenzidine was 35, 1.3, 35 × 106 KJ/mole and 1007 × 106 KJ/mole respectively. It obeyed Ping-Pong kinetics. The fluorescence intensities of the enzyme increased linearly as hydrogen peroxide concentration increased due to exposure of its hydrophobic moiety to the environment. Peel staining with Guaiacol substantiated it as a membrane-bound protein. Peroxidase was inhibited reversibly by various aromatic alcohols and its IC50 values were determined

Purification and characterization of membrane-bound <em>Borassus flabellifer</em> L. thermostable peroxidase

273-279Generating wealth out of waste is a goal in solid waste management. Borassus flabellifer shells with stone part are discarded as waste after removing its nutritious fruit. An enzyme extracted from the discarded stone part has potential application in molecular diagnosis and it is a wealth. The waste stone part bound Borassus flabellifer peroxidase was purified by salting-out, salting-in and DEAE-Cellulose anion exchange chromatographic technique to apparent homogeneity. Relative molecular weight under denaturating condition was around 40 kDa. The preparation had single isoenzyme as evidenced under nondenaturating condition. It was a glyco- and hemoprotein. It retained 100% activity for 120 h at 70°C. pH optima with benzidine, o-dianisidine, guaiacol and tetramethylbenzidine was around 5.0. Kinetic studies showed it had a higher affinity towards tetramethyl benzidine than other three substrates. The thermodynamic parameter of the enzyme with benzidine, o-dianisidine, guaiacol and tetramethylbenzidine was 35, 1.3, 35 × 106 KJ/mole and 1007 × 106 KJ/mole respectively. It obeyed Ping-Pong kinetics. The fluorescence intensities of the enzyme increased linearly as hydrogen peroxide concentration increased due to exposure of its hydrophobic moiety to the environment. Peel staining with Guaiacol substantiated it as a membrane-bound protein. Peroxidase was inhibited reversibly by various aromatic alcohols and its IC50 values were determined

Removal of Malachite Green from Aqueous Solution by Activated Carbon Developed from Cocoa (Theobroma Cacao) Shell - A Kinetic and Equilibrium Studies

The removal of malachite green (MG) by cocoa (Theobroma cacao) shell activated carbon (CSAC) was investigated in present study. Adsorption studies were performed by batch experiments as a function of process parameters such as initial pH, contact time, initial concentration and adsorbent dose. A comparison of kinetic models applied to the adsorption of MG on CSAC was evaluated for the pseudo-first order and pseudo-second order kinetic models. Results showed that the pseudo-second order kinetic model was found to correlate the experimental data well. The experimental equilibrium adsorption data was represented with Langmuir, Freundlich, Tempkin, Dubinin-Radushkevich and Flory-Huggins isotherms. The experimental data obtained in the present study indicated that activated carbon developed from cocoa shell can be attractive options for dye removal from waste water

Conformational Studies of Substituted Chromans: Crystal Structures of 2,2 '-(1E,1 ' E)-(2,3-Dimethylchroman-2,4-diyl)bis(Azan-1-yl-1-ylidene)bis(Methan-1-yl-1-ylidene)Diphenol (I) and 2,2 '-(1E,1 ' E)-(2-Ethyl-3-Methylchroman-2,4-diyl)bis(Azan-1-yl-1-ylidene)bis(Methan-1-yl-1-ylidene)Diphenol (II)

The title compounds C(25)H(24)N(2)O(3) (I) and C(26)H(26)N(2)O(3) (II) crystallize in the triclinic space group P-1 with cell parameters a = 8.981(1), b = 9.933(1), c = 12.369(2) , alpha = 78.537(2), beta = 84.515(2), gamma = 73.561(2)degrees Z = 2 (I) and a = 11.4630(9), b = 12.955(1), c = 16.154(1) , alpha = 70.425(1), beta = 87.403(1), gamma = 71.850(1)A degrees, Z = 4 (II). In both compounds the phenolic groups in the Schiff base substituents form intramolecular hydrogen bonds with the imine nitrogen atoms thereby rendering these substituents nearly planar. A detailed analysis of the amount by which the heterocyclic ring deviates from planarity (extent of puckering) in these and a series of related molecules shows that the extent of the deviation is largely unaffected by the number, size and placement of substituents on this ring