605 research outputs found

    Laboratory Focus on Improving the Culture of Biosafety: Statewide Risk Assessment of Clinical Laboratories That Process Specimens for Microbiologic Analysis

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    The Wisconsin State Laboratory of Hygiene challenged Wisconsin laboratories to examine their biosafety practices and improve their culture of biosafety. One hundred three clinical and public health laboratories completed a questionnaire-based, microbiology-focused biosafety risk assessment. Greater than 96% of the respondents performed activities related to specimen processing, direct microscopic examination, and rapid nonmolecular testing, while approximately 60% performed culture interpretation. Although they are important to the assessment of risk, data specific to patient occupation, symptoms, and travel history were often unavailable to the laboratory and, therefore, less contributory to a microbiology-focused biosafety risk assessment than information on the specimen source and test requisition. Over 88% of the respondents complied with more than three-quarters of the mitigation control measures listed in the survey. Facility assessment revealed that subsets of laboratories that claim biosafety level 1, 2, or 3 status did not possess all of the biosafety elements considered minimally standard for their respective classifications. Many laboratories reported being able to quickly correct the minor deficiencies identified. Task assessment identified deficiencies that trended higher within the general (not microbiology-specific) laboratory for core activities, such as packaging and shipping, direct microscopic examination, and culture modalities solely involving screens for organism growth. For traditional microbiology departments, opportunities for improvement in the cultivation and management of highly infectious agents, such as acid-fast bacilli and systemic fungi, were revealed. These results derived from a survey of a large cohort of small- and large-scale laboratories suggest the necessity for continued microbiology-based understanding of biosafety practices, vigilance toward biosafety, and enforcement of biosafety practices throughout the laboratory setting

    Resilience of the Dutch HPV-based cervical screening programme during the COVID-19 pandemic

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    Objectives:ย Organisation of a screening programme influences programme resilience to a disruption as COVID-19. Due to COVID-19, the Dutch human papillomavirusโ€“based cervical screening programme was temporarily suspended. Afterwards, multiple measures have been taken to catch-up participation. This study aimed to investigate programme resilience by examining the effect of COVID-19 and programme measures taken on participation in cervical screening.ย Study design:ย Observational cohort study.ย Methods:ย Data from the national screening registry and Dutch nationwide pathology databank (Palga) were used on invitations and follow-up in 2018/2019 (pre-COVID) and 2020 (COVID). Sending invitations, reminders and self-sampling kits were suspended from March to July 2020. Main outcome measures include distribution of participant characteristics (age, region and screening history), participation rates by age and region, time between invitation and participation (i.e. response time) and self-sampling use per month.ย Results:ย Participation rate was significantly lower in 2020 (49.8%) compared to 2018/19 (56.8%, P &lt; 0.001), in all ages and regions. Compared to 2018/19, participation rates decreased most in women invited from January to March 2020 (โˆ’6.7%, โˆ’9.1% and โˆ’10.4%, respectively). From August, participation rates started to recover (difference between โˆ’0.8% and โˆ’2.7%). Median response time was longer in February and March (2020: 143 and 173 days; 2018/19: 53 and 55 days) and comparable from July onwards (median difference 0โ€“6 days). Self-sampling use was higher in 2020 (16.3%) compared to 2018/19 (7.6%).ย Conclusions:ย The pandemic impacted participation rates in the Dutch cervical screening programme, especially of women invited before the programme pause. Implementation of self-sampling in national cervical screening programmes could increase participation rates and could serve as an alternative screening method in times of exceptional health care circumstances, such as a pandemic. Due to the well-organised programme and measures taken to catch-up participation, the impact of COVID-19 on the screening programme remained small.</p

    Phosphorus bioavailability in soil profiles of a long-term fertilizer experiment: The evaluation of their bioaccessibility

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    Global agricultural productivity depends on the use of finite phosphorus (P) resources of which not only the topsoil, but also subsoil, can hold immense reserves. To assess potential soil contribution to plant nutrition, we compared the P status of Stagnic Cambisol profiles in experimental plots that received different P fertilizer applications (control, triple superphosphate (TSP), compost, compost+TSP) for 16 years. Sequential fractionation was combined with P K-edge X-ray absorption near edge structure (XANES) spectroscopy to identify the chemical P speciation. Fertilized topsoils (21 to 69 kg P ha-1 a-1) showed P reserves larger by a factor of 1.2 to 1.4, and subsoil P reserves larger by a factor of 1.3 to 1.5 than those of the control. P-XANES revealed the predominance of inorganic P species such as moderately labile Fe- (46 to 92%), Al- (0 to 40%), and Ca- (0 to 15%) P compounds besides organic P (0 to 13%) in all treatments. The fertilizer application slightly altered P speciation throughout the profiles, but the type of fertilizer had no significant effect on it. Optimal plant growth requirements are restricted by the exchangeable P from the solid phase within the soil solution. Therefore, ongoing research focuses on the accessibility of P from P loaded amorphous Fe- and Al-hydroxides, previously identified as the predominant abiotic P forms. To assess their P desorption potential, P-33 rhizotron experiments combined with P-33 isotopic exchange kinetics (IEK) are underway. Preliminary results indicated that besides differences in P binding capacity of soil hydroxides, physical soil parameters, such as the matric potential, strongly control soil P availability, thus plant P acquisition rates can vary among different soil types. Our results gained new detailed information about P bioavailability under agricultural practice. The investigations towards P bioaccessibility may contribute to improved interpretation of soil P tests and reduced fertilizer recommendations

    Towards the development of a simulator for investigating the impact of people management practices on retail performance

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    ๏€Œ๏€‰๏€ž๏€„๏€‚ ๏€ˆ๏€„๏€๏€„๏€ˆ๏€‚ ๏€ ๏€Š๏€‚ ๏€‰๏€๏€Ž๏€ž๏€…๏€‰๏€“๏€ž๏€Œ๏€ ๏€•๏€‚ ๏€Š๏€ ๏€…๏€‚ ๏€‰๏€‚ ๏€Ž๏€ฃ๏€„๏€“๏€Œ๏€Š๏€Œ๏€“๏€‚ ๏€‰๏€ฃ๏€ฃ๏€ˆ๏€Œ๏€“๏€‰๏€ž๏€Œ๏€ ๏€•๏€‚ ๏€Œ๏€Ž๏€‚ ๏€ ๏€Š๏€ž๏€„๏€•๏€‚ ๏€ข๏€ ๏€…๏€„๏€‚ ๏€ ๏€Š๏€‚ ๏€‰๏€•๏€‚ ๏€‰๏€…๏€ž๏€‚ ๏€ž๏€™๏€‰๏€•๏€‚ ๏€‰๏€‚\ud ๏€Ž๏€“๏€Œ๏€„๏€•๏€“๏€„๏€›๏€‚๏€š๏€„๏€‚ ๏€™๏€‰๏€๏€„๏€‚ ๏€ฎ๏€„๏€๏€„๏€ˆ๏€ ๏€ฃ๏€„๏€ฎ๏€‚ ๏€‰๏€‚ ๏€…๏€„๏€ž๏€‰๏€Œ๏€ˆ๏€‚ ๏€๏€…๏€‰๏€•๏€“๏€™๏€‚ ๏€Ž๏€Œ๏€ข๏€ค๏€ˆ๏€‰๏€ž๏€Œ๏€ ๏€•๏€‚๏€ข๏€ ๏€ฎ๏€„๏€ˆ๏€‚ ๏€ž๏€ ๏€‚ ๏€Œ๏€•๏€๏€„๏€Ž๏€ž๏€Œ๏€œ๏€‰๏€ž๏€„๏€‚๏€‘๏€™๏€Œ๏€“๏€™๏€‚ ๏€ˆ๏€„๏€๏€„๏€ˆ๏€‚ ๏€ ๏€Š๏€‚\ud ๏€ข๏€ ๏€ฎ๏€„๏€ˆ๏€‚๏€‰๏€“๏€“๏€ค๏€…๏€‰๏€“๏€Ÿ๏€‚๏€Œ๏€Ž๏€‚๏€…๏€„๏€ณ๏€ค๏€Œ๏€…๏€„๏€ฎ๏€‚๏€Š๏€ ๏€…๏€‚๏€Ž๏€ค๏€“๏€™๏€‚๏€‰๏€‚๏€ข๏€ ๏€ฎ๏€„๏€ˆ๏€‚๏€ž๏€ ๏€‚๏€ ๏€๏€ž๏€‰๏€Œ๏€•๏€‚๏€ข๏€„๏€‰๏€•๏€Œ๏€•๏€œ๏€Š๏€ค๏€ˆ๏€‚๏€…๏€„๏€Ž๏€ค๏€ˆ๏€ž๏€Ž๏€‚๏€Š๏€ ๏€…๏€‚๏€ฃ๏€…๏€‰๏€“๏€ž๏€Œ๏€ž๏€Œ๏€ ๏€•๏€„๏€…๏€Ž๏€›

    Integrating Slacks-based Measure of Efficiency and Super-efficiency in Data Envelopment Analysis

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    In this paper, we develop an integrated model for slacks- based measure (SBM) simultaneously of both the efficiency and the super-efficiency for decision-making units (DMUs) in data envelopment analysis (DEA). Unlike the traditional solution approaches in which we need to identify the efficient DMUs by the SBM model of Tone [20] before applying the super SBM model of Tone [21] for the DMUs to achieve their super-effciency scores, our integration can obtain the efficiency scores of the infficient DMUs and the super-efficiency scores of the efficient DMUs by solving simultaneously these two models by an onestage approach. Therefore, it may save computational time for large-scale practical applications. Due to the non-linearity in the objective function of this integrated model, we develop a linearisation technique to deal with the non-linear model. The numerical experiments, carried out on several examples in the literature and a case study, have demonstrated the accuracy and the computational time effectiveness of our proposed model as compared with the traditional solution approache
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