Ultrasonic study of 5H-dibenz[B,F]azepine-5-carboxamide and 7-chloro-1,5-dihydro-1-methyl-5-phenyl-1,5-benzodiazepine-2,4(3H)-dione

241-244The experimental ultrasonic velocity has been measured for anticonvulsants such as Carbamazepine(CBZ), Oxycarbazepine(OXYCBZ) and Clobazam. The drugs are some of the most preferred for almost any kind of seizure. The highly non-soluble nature of such drugs in water might have not encouraged people to estimate the speed of sound through these drugs. In the present paper, based on the solubility of these drugs in DMSO (dimethylsulphoxide), water is gradually added to obtain different concentrations. The sound velocity is measured using 1 and 3 MHz interferometer specifically at room temperature. The sound velocity data is used to estimate the structural parameter which in turn exposes the similarity in behaviour of all the three drugs

Characterization of the biochemical and biophysical properties of the Saccharomyces cerevisiae phosphate transporter Pho89

In Saccharomyces cerevisiae, Pho89 mediates a cation-dependent transport of Pi across the plasma membrane. This integral membrane protein belongs to the Inorganic Phosphate Transporter (PiT) family, a group that includes the mammalian Na+/Pi cotransporters Pit1 and Pit2. Here we report that the Pichia pastoris expressed recombinant Pho89 was purified in the presence of Foscholine-12 and functionally reconstituted into proteoliposomes with a similar substrate specificity as observed in an intact cell system. The alpha-helical content of the Pho89 protein was estimated to 44%. EPR analysis showed that purified Pho89 protein undergoes conformational change upon addition of substrate. © 2013 The Authors