57 research outputs found
Endometrial tumor invasiveness is related to metalloproteinase 2 and tissue inhibitor of metalloproteinase 2 expressions.
Matrix metalloproteinase (MMPs) expression has been linked to gynecological tumor aggressiveness. The objective of this study was to determine MMP-2, MMP-7, and MMP-9 and tissue inhibitors of metalloproteinases (TIMP)-1 and TIMP-2 expression in endometrial malignancies and their relation to clinical and histologic parameters. Formalin-fixed, paraffin-embedded tumor samples from 50 patients with endometrial carcinoma treated between 1999 and 2004 were stained with specific monoclonal antibodies. The tumors were grouped according to the FIGO classification. The staining results were compared to histologic and clinical data. Semiquantitative analysis of MMP and TIMP expression showed a significant difference in TIMP-2 expression according to the histologic subtype (P = 0.03) and also a trend towards a difference in MMP-9 expression (P = 0.05). MMP-2 expression increased and TIMP-2 expression fell as the histologic grade increased (P = 0.0007, P < 0.0001, respectively). MMP-2 expression correlated with lymph node metastasis (P = 0.04), while TIMP-2 expression correlated with the depth of myometrial invasion (P = 0.01), vasculolymphatic space involvement (P = 0.02), and lymph node metastasis (P = 0.0003). These results support the involvement of MMPs and TIMPs in endometrial tumor growth and progression. High MMP-2 and low TIMP-2 expression were the most potent markers of endometrial tumors with a high risk of local and distant spread
Usefulness of HPV testing in the follow-up of untreated cervical low grade lesions
The aim of the present work was to evaluate
the usefulness of high-risk human papillomavirus (HRHPV)
testing for the follow-up of women with untreated
low grade cervical squamous cell lesions (LSIL). For
that, 412 women with a cytological diagnosis of LSIL at
entry were monitored by cytology, HR-HPV testing with
the Hybrid Capture II assay (HC-II) and colposcopy. Our
primary endpoint was clinical progression defined by the
presence of a high grade cervical intraepithelial
neoplasia (CIN2 and CIN3) at the biopsy. At baseline,
histological control revealed 10 CIN2 and 11 CIN3 only
in the cohort of women HR-HPV+. In the follow-up, 4
CIN2 and 8 CIN3 were detected, always in the women
initially HR-HPV+. Thus, the recurrence of a HR-HPV+
infection clearly selects a population at high-risk for
CIN2-3. The semi-quantitative appreciation of the viral
load with HC-II could not be used as a good prognostic
factor for the follow-up of women with LSIL. HR-HPV
testing reduces the number of cytology and colposcopy
examinations in the follow-up of women aged >35 years
when HPV testing is initially negative. Thus HR-HPV
testing should be reserved for the follow-up of this
population of women initially HR-HPV+ and proposed 6
to 12 months after the cytological diagnosis of LSIL
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