Environmental effects on galaxy evolution. II: quantifying the tidal features in NIR-images of the cluster Abell 85

This work is part of a series of papers devoted to investigate the evolution of cluster galaxies during their infall. In the present article we imaged in NIR a selected sample of galaxies through- out the massive cluster Abell 85 (z = 0.055). We obtained (JHK) photometry for 68 objects, reaching 1 mag/arcsec^2 deeper than 2MASS. We use these images to unveil asymmetries in the outskirts of a sample of bright galaxies and develop a new asymmetry index, alpha_An, which allows to quantify the degree of disruption by the relative area occupied by the tidal features on the plane of the sky. We measure the asymmetries for a subsample of 41 large area objects finding clear asymmetries in ten galaxies, most of them being in groups and pairs projected at different clustercentric distances, some of them located beyond R500 . Combining information on the Hi-gas content of blue galaxies and the distribution of sub-structures across Abell 85, with the present NIR asymmetry analysis, we obtain a very powerful tool to confirm that tidal mechanisms are indeed present and are currently affecting a fraction of galaxies in Abell 85. However, when comparing our deep NIR images with UV-blue images of two very disrupted (jellyfish) galaxies in this cluster, we discard the presence of tidal 1 interactions down to our detection limit. Our results suggest that ram-pressure stripping is at the origin of such spectacular disruptions. We conclude that across a complex cluster like Abell 85, environment mechanisms, both gravitational and hydrodynamical, are playing an active role in driving galaxy evolution.Comment: 30 pages, 13 figures, Accepted for Publication in A

Evaluation of different methods for the characterization of carrot resistance to the alternaria leaf blight pathogen (Alternaria dauci) revealed two qualitatively different resistances

Alternaria leaf blight (ALB), caused by Alternaria dauci, is one of the most damaging foliar diseases of carrot worldwide. The aim of this study was to compare different methods for evaluating levels of carrot resistance to ALB. Three techniques were investigated by comparison with a visual disease assessment control: in vivo conidial germination, a bioassay based on a drop-inoculation method, and in planta quantification of fungal biomass by quantitative PCR (Q-PCR). Three carrot cultivars showing different degrees of resistance to A. dauci were used, i.e. a susceptible cultivar (Presto) and two partially resistant genotypes (Texto and Bolero), challenged with an aggressive or a very aggressive isolate of A. dauci. Both partially resistant genotypes produced a higher mean number of germ tubes per conidium (up to 3·42±0·35) than the susceptible one (1·26±0·18). The drop-inoculation results allowed one of the partially resistant genotypes (Bolero, log10(S+1) = 1·34±0·13) to be distinguished from the susceptible one (1·90±0·13). By contrast, fungal growth measured by Q-PCR clearly differentiated the two partially resistant genotypes with log10(I) values of 2·77±0·13 compared to the susceptible cultivar (3·65±0·13) at 15 days post-inoculation. This result was strongly correlated (r2 = 0·91) with the disease severity index scored at the same date. Data obtained with the different assessment methods strongly suggest that the Texto and Bolero genotypes have different genetic resistance sources

Is quantitative resistance qualitative? An example with two Alternaria Leaf Blight resistant carrot genotypes and four resistance assessment techniques

International audienc

Evaluating aggressiveness and host range of Alternaria dauci in a controlled environment

The aggressiveness of Alternaria dauci isolates was investigated in greenhouse conditions. Twenty-seven isolates were pre-selected from a large collection to represent high diversity according to geographic or host origins and intergenic spacer (IGS) polymorphism. IGS sequence analysis revealed that isolates were grouped within three different clusters. Eleven isolates were selected and inoculated on a susceptible carrot cultivar. Three criteria (mean lesion number, mean necrotic leaf area and mean disease index) were used to assess the aggressiveness of isolates. Continuous variation in aggressiveness was shown and no clear division into isolate classes was evident. For the host range study, two isolates were inoculated under greenhouse conditions onto nine cultivated Apiaceae species, two wild Daucus species and six cultivated non-Apiaceae species representing six botanical families. Lesions varying in severity were observed on all dicot species (Apiaceae and non-Apiaceae), but no symptoms developed on the two monocots studied (leek and sweetcorn). Plant species were also differentiated on the basis of expanding lesions (cultivated and wild carrot, dill and fennel) or non-expanding lesions (other dicot species). Typical A. dauci conidia were observed after in vitro incubation of leaves with symptoms. Fungal structures were isolated from lesions and A. dauci was confirmed on the basis of conidial morphology and specific conventional PCR results. Genotyping of individual isolates performed with microsatellite markers confirmed the presence of the inoculated isolate. The results clearly showed that, in controlled conditions, the host range of A. dauci is not restricted to carrot