Temporal dynamics of sucking pest and field response of promising insecticidal molecules in okra

To investigate the response due to application of newer insecticide on sucking pest in okra, a trial was designed at field level for three consecutive years from 2011-12 to 2013-14 in kharif season. Moreover, impacts of applied insecticides on natural enemies were also assessed. Based on experimental finding thiamethoxam 25WG 0.003% (2.83 per 3 leaves, 0.93 per 3 leaves), imidacloprid 70WG 0.004% (3.49 per 3 leaves, 1.30 per 3 leaves) and thiacloprid 21.7 SC 0.006% (4.28 per 3 leaves, 1.75 per 3 leaves) provided superior control of leafhoppers and whiteflies population on okra. Effectiveness of these treatments was reflected in terms of reduction in population of both insects and significantly increases (thiamethoxam: 95.50 q/ha, imidacloprid: 86.96 q/ha and thiacloprid: 80.99 q/ha) the fruit yield in comparison to others. However, the incidence of Yellow Vein Mosaic disease was recorded least in thiamethoxam 0.003% sprayed plots followed by imidacloprid 0.004%. Slow progress in the population ofwhitefly and leaf hopper was recorded in thiamethoxam 0.003% applied plots. There was positive correlation between whitefly and virus incidence in conducted field trial. Under the experiment, neonicotinoids group of insecticides have not adverse effect on natural enemies in okra crop. The information generated under the study can be incorporated in management modules in crop okra without disturbing the ecology of natural enemy and cropping system. In our findings, the quantitative data of temporal increment of whiteflies and mosaic disease will be helpful in understanding or formulating of epidemiological models

Field dissipation of pendimethalin and alachlor in sandy clay loam soil and its terminal residues in sunflower (Helianthus annus L.)

Field experiments were conducted with sunflower as a test crop during 2010-11 to study the dissipation kinetics and the persistence of pendimethalin and alachlor in sandy clay loam soil and its terminal residues in sunflower. Herbicides were applied at recommended and double the recommended dose along with control and the treatments were replicated thrice in randomized block design. The soil and plant samples collected at periodical intervals for herbicides residue determination using GC equipped with ECD detector. Results shows that the degradation of both the herbicides in soil was faster at higher dose of application than at the lower dose and the concentration decreased with the advancement in crop growth. While pendimethalin persisted in soil for 60 – 90 days, the alachlor persisted in soil for 30 - 45 days depending on the quantity of application. Degradation of both the herbicides in soil followed first order kinetics with the mean half life of 14.6 and 9.8 days respectively for pendimethalin and alachlor. Residues of these herbicides were below 0.001 mg/kg at the time of harvest in soil, sunflower seeds and stalks showed that these herbicides a can be safely used for the control of weeds in sunflower cultivation

Influence of surface metallic silver deposit and surface fluorination on the photocatalytic activity of rutile TiO2 for the degradation of crystal violet a cationic dye under UV light irradiation

Silver metallization and fluorination on the surface of rutile TiO2 (SRT and FRT) was carried out by photochemical reduction and wet impregnation methods respectively. TiO2, SRT and FRT were characterized by various analytical techniques like PXRD, SEM, EDX, FTIR, PL, UV�vis absorbance and XPS. The photocatalytic degradation of crystal violet (CV) was carried out at three different pH conditions. Acidic pH was found to be more favorable for the degradation, in spite of low adsorption of cationic CV molecules on the catalyst surface. The electrostatic repulsion at this pH drives the CV molecules into the bulk of the solution suggesting the involvement of bulk hydroxyl radicals rather than surface adsorbed hydroxyl radicals. The degradation efficiency can be represented by the following order SRT > FRT > TiO2. The Ti-peroxo (Ti-OO-Ti) complex species formed in presence of H2O2 by the combination of two trapped holes (Ti�Orad) in aqueous medium is predicted to enhance the rate of generation of hydroxyl radicals. The exciton mobility is dependent on the polaron effective mass which is higher for rutile TiO2 accounting for its lower activity. The bulk charge carrier transport which is less in bare rutile TiO2 is enhanced in the surface modified TiO2. Effective trapping of photogenerated excitons/electrons by F-/Ag0 can facilitate their migration and increase the activity by tenfolds. The rate of degradation of CV followed two reaction pathways, slower N-demethylation in basic conditions and rapid aromatic cleavage at the central carbon atom in the acidic pH conditions. © 201

Behaviour of non-donor specific antibodies during rapid re-synthesis of donor specific HLA antibodies after antibody incompatible renal transplantation

Background: HLA directed antibodies play an important role in acute and chronic allograft rejection. During viral infection of a patient with HLA antibodies, the HLA antibody levels may rise even though there is no new immunization with antigen. However it is not known whether the converse occurs, and whether changes on non-donor specific antibodies are associated with any outcomes following HLA antibody incompatible renal transplantation. Methods: 55 patients, 31 women and 24 men, who underwent HLAi renal transplant in our center from September 2005 to September 2010 were included in the studies. We analysed the data using two different approaches, based on; i) DSA levels and ii) rejection episode post transplant. HLA antibody levels were measured during the early post transplant period and corresponding CMV, VZV and Anti-HBs IgG antibody levels and blood group IgG, IgM and IgA antibodies were quantified. Results: Despite a significant DSA antibody rise no significant non-donor specific HLA antibody, viral or blood group antibody rise was found. In rejection episode analyses, multiple logistic regression modelling showed that change in the DSA was significantly associated with rejection (p = 0.002), even when adjusted for other antibody levels. No other antibody levels were predictive of rejection. Increase in DSA from pre treatment to a post transplant peak of 1000 was equivalent to an increased chance of rejection with an odds ratio of 1.47 (1.08, 2.00). Conclusion: In spite of increases or decreases in the DSA levels, there were no changes in the viral or the blood group antibodies in these patients. Thus the DSA rise is specific in contrast to the viral, blood group or third party antibodies post transplantation. Increases in the DSA post transplant in comparison to pre-treatment are strongly associated with occurrence of rejection

Signal Appropriation of Explicit HIV Status Disclosure Fields in Sex-Social Apps used by Gay and Bisexual Men

HIV status disclosure fields in online sex-social applications ("apps") are designed to help increase awareness, reduce stigma, and promote sexual health. Public disclosure could also help those diagnosed relate to others with similar statuses to feel less isolated. However, in our interview study (n=28) with HIV positive and negative men who have sex with men (MSM), we found some users preferred to keep their status private, especially when disclosure could stigmatise and disadvantage them, or risk revealing their status to someone they knew offline in a different context. How do users manage these tensions between health, stigma, and privacy? We analysed our interview data using signalling theory as a conceptual framework and identify participants developing 'signal appropriation' strategies, helping them manage the disclosure of their HIV status. Additionally, we propose a set of design considerations that explore the use of signals in the design of sensitive disclosure fields

Rapid, ultra low coverage copy number profiling of cell-free DNA as a precision oncology screening strategy.

Current cell-free DNA (cfDNA) next generation sequencing (NGS) precision oncology workflows are typically limited to targeted and/or disease-specific applications. In advanced cancer, disease burden and cfDNA tumor content are often elevated, yielding unique precision oncology opportunities. We sought to demonstrate the utility of a pan-cancer, rapid, inexpensive, whole genome NGS of cfDNA approach (PRINCe) as a precision oncology screening strategy via ultra-low coverage (~0.01x) tumor content determination through genome-wide copy number alteration (CNA) profiling. We applied PRINCe to a retrospective cohort of 124 cfDNA samples from 100 patients with advanced cancers, including 76 men with metastatic castration-resistant prostate cancer (mCRPC), enabling cfDNA tumor content approximation and actionable focal CNA detection, while facilitating concordance analyses between cfDNA and tissue-based NGS profiles and assessment of cfDNA alteration associations with mCRPC treatment outcomes. Therapeutically relevant focal CNAs were present in 42 (34%) cfDNA samples, including 36 of 93 (39%) mCRPC patient samples harboring AR amplification. PRINCe identified pre-treatment cfDNA CNA profiles facilitating disease monitoring. Combining PRINCe with routine targeted NGS of cfDNA enabled mutation and CNA assessment with coverages tuned to cfDNA tumor content. In mCRPC, genome-wide PRINCe cfDNA and matched tissue CNA profiles showed high concordance (median Pearson correlation = 0.87), and PRINCe detectable AR amplifications predicted reduced time on therapy, independent of therapy type (Kaplan-Meier log-rank test, chi-square = 24.9, p < 0.0001). Our screening approach enables robust, broadly applicable cfDNA-based precision oncology for patients with advanced cancer through scalable identification of therapeutically relevant CNAs and pre-/post-treatment genomic profiles, enabling cfDNA- or tissue-based precision oncology workflow optimization

Study Of Energy Efficient And Secure Routing Mechanisms In Wireless Sensor Network

Wireless Sensor Network (WSN) is a group of Sensor Nodes (SN) correspond with each other and sharing the information between the networks. SN are low power and low cost devices are used to construct the network. Sensor based networks are used in various integrated applications. One of the most benefited sensor based application is Healthcare applications. In Healthcare applications, the physical components are data sources and collected information’s are transmitted through the network in the mode of intermediate node access. All the physical components or sensors are battery powered in nature, so the energy was play a vital role in Healthcare-WSN (HWSN) applications. Nodes energy will decide the utilisation of the network and accurate data transmission between the nodes. The future of WSN is purely dependent on the Energy saving mechanism and now it is create a new dimension of research constraint. Now, the researchers focusing on the Energy efficient and secure techniques for improve the lifetime of the network and safest data transmission between the nodes and the server. In this paper, various energy efficient mechanisms are analyzed for prolong the network and also review the various secure mechanisms to improve the security of the HWSN applications