Respiratory burst by dengue-virus-induced cytotoxic factor

This study investigates the induction and release of the superoxide anion (O- 2) and hydrogen peroxide (H2O2) by mouse spleen cells on stimulation with dengue type 2 virus (DV) and a DV-induced cytokine, the cytotoxic factor (mCF). Methods: Normal mice or their spleen cell cultures were inoculated with DV or mCF. At different time periods, the spleen cell supernatants were assayed for the production of O- 2 and H2O2. Results: Inoculation of DV in spleen cell cultures resulted in peak production of O- 2 and H2O2 at 48 and 72 h, respectively, while in DV-infected mouse spleen, the maximum production was on days 7 and 8, which correlated with the appearance of mCF in the milieu. Maximum O- 2 and H2O2 production occurred at 45 min and 1 h after inoculation of 5 µ g of mCF. Pretreatment of mCF with anti-mCF-antiserum inhibited O- 2 and H2O2 release indicating the specificity of the induction by mCF. The enriched subpopulations of macrophages and T cells produced O- 2 and H2O2 and not B cells. Treatment of the cells with superoxide dismutase increased H2O2 release but inhibited O- 2 release and the cytotoxicity in a dose-dependent manner. Conclusion: This showed that O- 2 is responsible for the cytotoxic activity of mCF and not H2O2. In conjunction with our earlier findings that pretreatment with NG-monomethyl-L-arginine inhibited mCF-induced production of NO and the cytotoxicity, it is concluded that the presence of both O- 2 and NO is required for the cytotoxic activity of mCF, thereby indicating a possible role of peroxynitrite

Active immunization by a dengue virus-induced cytokine

Dengue type 2 virus (DV)-induced cytotoxic factor (CF) is capable of reproducing various pathological lesions in mice that are seen in human dengue. The present study was undertaken to investigate the protective effect of active immunization of mice with CF, Mice were immunized with 5 μ g of CF and prevention of CF-induced increase in capillary permeability and damage to the blood brain barrier were studied at weekly intervals, up to 48 weeks, by challenging with 3 μ g of CF, Maximum protection against increase in capillary permeability and damage to the blood-brain barrier was observed in week 4 after immunization. A breakthrough in the protection occurred with higher doses of CF in a dose-dependent manner. Challenge with a lethal intracerebral (i.e.) dose of DV showed significantly prolonged mean survival time and delayed onset of symptoms of sickness in the immunized mice compared with the normal mice, but the titre of the virus in the brain was similar in the two groups. On i.p. challenge with the virus the protection against damage to the blood-brain barrier was 86± 7% at week 4 and 17± 4% at week 26 after immunization. Sera obtained from the immunized mice showed the presence of CF-specific antibodies by ELISA, Western blot, and by neutralization of the cytotoxic activity of CF in vitro. The present study describes successful prevention of a cytokine-induced pathology by specific active immunization

Estimation of a Monotone Mean Residual Life

In survival analysis and in the analysis of life tables an important biometric function of interest is the life expectancy at age x,M(x), defined by M(x)=E[X?x|X\u3ex], where X is a lifetime. M is called the mean residual life function. In many applications it is reasonable to assume that M is decreasing (DMRL) or increasing (IMRL); we write decreasing (increasing) for nonincreasing (non-decreasing). There is some literature on empirical estimators of M and their properties. Although tests for a monotone M are discussed in the literature, we are not aware of any estimators of M under these order restrictions. In this paper we initiate a study of such estimation. Our projection type estimators are shown to be strongly uniformly consistent on compact intervals, and they are shown to be asymptotically root-n equivalent in probability to the (unrestricted) empirical estimator when M is strictly monotone. Thus the monotonicity is obtained free of charge , at least in the aymptotic sense. We also consider the nonparametric maximum likelihood estimators. They do not exist for the IMRL case. They do exist for the DMRL case, but we have found the solutions to be too complex to be evaluated efficiently

Analysis of physical-chemical processes governing SSME internal fluid flows

The efforts to adapt CHAM's computational fluid dynamics code, PHOENICS, to the analysis of flow within the high pressure fuel turbopump (HPFTP) aft-platform seal cavity of the SSME are summarized. In particular, the special purpose PHOENICS satellite and ground station specifically formulated for this application are listed and described, and the preliminary results of the first part two-dimensional analyses are presented and discussed. Planned three-dimensional analyses are also briefly outlined. To further understand the mixing and combustion processes in the SSME fuelside preburners, a single oxygen-hydrogen jet element was investigated

A non destructive method to detect the incipient fault in rolling element bearing

It is essential to have prior warning of incipient fault (s) in any critical equipment occurring due to vibration. Vibration monitoring is good nondestructive technique to detect the fault of machines at an early stage. Capturing vibration signature of machines at slow speed is a difficult task due to impact of low energy, mostly absorbed by the structural path and falls much below the cut off frequency of the normally available accelerometer. To resolve the issue, a systematic proce-dure has been developed. It has the facility to break the signal in different level, envelope capturing and finally convert to frequency spectrum. The system has been tested under various operating condition that suits to industry to diagnose the problem of bearing. The method can predict the problem very well at its inception

Cytotoxic factor in dengue haemorrhagic fever

Objective: Cytotoxic factor is a unique pathogenesis-associated cytokine that is produced in mice (mCF) and man (hCF) during dengue virus infection. This study was undertaken to investigate the prevalence of hCF and its relationship to the duration and severity of the illness, and to ascertain its role, if any, in the pathogenesis of dengue fever (DF) and dengue haemorrhagic fever (DHF). Methods: Peripheral venous blood was collected from the patients of various grades and on different days after the onset of clinical illness. Sera were collected from a total of 333 cases, and analysed for the presence of hCF by inhibition ELISA and dot blot tests. Result: The positivity for hCF was 100% in cases of DHF grades III and IV, while overall positivity was seen in 295 out of 333 (88%) cases studied. Sera collected from the 1st to the 20th day of illness were positive for hCF. This was not seen at later periods. A majority of cases (52%) were below 15 years of age and peak positivity of 96% was noticed in the age-group of 11-15 years. The mean inhibition value of the ELISA was lowest (40 ± 8%) in cases of DF and was highest (70 ± 10%) in DHF grade IV, and the peak titres were found on the first 4 days of the illness. Conclusion: The findings show the presence of hCF in the majority of cases. It is detectable up to the 20th day of illness and may suggest an association of higher levels of hCF with the onset and severity of the illness

IXAE observations of the X-ray pulsar XTE J1946+274

We report results from two observations of the transient binary X-ray pulsar XTE J1946+274 with the Indian X-ray Astronomy Experiment (IXAE) made in September 18-30 1999 and June 28-July 7 2000. The pulsar has a ~80 days orbital period during which the X-ray intensity varies almost sinusoidally between intensity levels of 5 and 50 mCrab. The mid-time of the two observations with the IXAE are separated by an orbital phase of 0.56 and were in the decaying and rising parts of its orbital modulation, respectively. During both the observations, strong pulsations with 15.8 s period are detected in the 2-6 and 6-18 keV energy bands of IXAE. The pulse profiles in both the observations are double peaked and identical with a similar pulse fractions of ~30% in the two energy bands. The pulse periods and its local derivatives are obtained from the IXAE observations. The known pulse period history shows small deviations from an otherwise nearly constant spin-up trend of the pulsar since its discovery. If the measured local period derivatives are a sum of a nearly constant spin-up of the pulsar and effect of its orbital motion, it suggests that the orbit of this Be X-ray binary is eccentric. The RXTE-ASM light curve shows a continuous detection of 10 orbital modulation of the source since its discovery

Luminosity dependent changes in the X-ray pulse profile of the transient pulsar Cepheus X-4 during its declining phase of the 1997 outburst

The transient X-ray pulsar Cepheus X-4 underwent its latest outburst in 1997, during July-August, which lasted for about 30 days. The Pointed mode Proportional Counters (PPCs) of the Indian X-ray Astronomy Experiment (IXAE) on board IRS-P3 satellite observed the source in its declining phase during 1997 July 28 to July 30. The timing analysis of the data confirms the 66 seconds pulsation of the neutron star. The X-ray pulse profile obtained in two energy bands between 2 and 18 keV, shows energy dependent variations. The pulse profile obtained by us in the declining phase of the outburst when the X-ray luminosity of the source was about 6x1035 ergs s-1, is distinctly different from the one observed with the RXTE in the earlier phase of the outburst. It is found that near the end of the outburst, the relative strength of the two pulses of the double-pulse profile got reversed and the inter-pulse became more dominant compared to the main pulse. The observations can be interpreted in terms of a luminosity dependent emission profile of the pulsar, where depending on the pulsar geometry with respect to line of sight, one of the emission patterns, either a pencil-beam or a fan-beam, becomes more dominant. This is due to the changes in the pulsar magneto-sphere below a specific luminosity, which may cause relative changes in accretion process onto the two poles of the neutron star

Hidden Sp(2s+1)- or SO(2s+1)-symmetry and new exactly solvable models in ultracold atomic systems

The high spin ultracold atom models with a special form of contact interactions, i.e., the scattering lengthes in the total spin-$2,4 \cdots$ channels are equal but may be different from that in the spin-0 channel, is studied. It is found that those models have either $Sp(2s+1)$-symmetry for the fermions or $SO(2s+1)$-symmetry for the bosons in the spin sector. Based on the symmetry analysis, a new class of exactly solvable models is proposed and solved via the Bethe ansatz. The ground states for repulsive fermions are also discussed.Comment: 6 pages, 2 figure