Response of \u3cem\u3eAxonopus catarinensis\u3c/em\u3e and \u3cem\u3eArachis pintoi\u3c/em\u3e to Shade Conditions

In the north-east of Argentina, there are more than 100,000 hectares of silvopastoral systems where trees, forages and livestock are combined with the goal to diversify income, reduce financial risk, obtain more profit and enhance environmental benefit (Cubbage et al. 2013). The rapid adoption of this production system by farmers has generated high demand for information on shade tolerant grass and legume forage species. Axonopus catarinensis is a native grass from Itajai Vally (Brazil) that was introduced to the north-east of Argentina 10 years ago; whereas Arachis pintoi is a sub-tropical legume (also native to Brazil) adapted to acid soils and tolerant of medium levels of shade (Fisher and Cruz 1994). Visual observation of these species in the field indicated high yields and acceptable tolerance to shade. A trial was subsequently carried out with the aim to quantify dry matter yield and nutritive quality of the species under different levels of shade for silvopastoral use

Expression, purification and analysis of the anti-HIV Cyanovirin-N produced in transgenic soybeans seeds.

Edição do Congress of the Brazilian Biotechnology Society, Florianópolis, 2013

Application of Electrochemically Produced Aluminum Hydroxide Gel for Prepurification of Recombinant Synthetic Green Fluorescent Protein Produced in Tobacco Leaves

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)The use of recombinant proteins has increased greatly in recent years, as also have increased the number of techniques and materials used for their production and purification. Among the different types of bioreactors being studied, there is a general consensus among scientists that production in green plant tissues such as leaves is more feasible. However, the presence of chlorophyll and phenolic compounds in plant extracts, which can precipitate and denature the proteins besides damaging separation membranes and gels, makes this technology impracticable on a commercial scale. In the present work, the adsorption to electrochemically produced aluminum hydroxide gel was applied as a prepurification step for recombinant synthetic green fluorescent protein (sGFP), also referred to as enhanced green fluorescent protein, produced in Nicotiana benthamiana leaves. Removal efficiencies of 99.7% of chlorophyll, 88.5% of phenolic compounds, and 38.5% of native proteins from the N. benthamiana extracts were achieved without removing sGFP from the extracts. As electrochemical preparation of aluminum hydroxide gel is a cost-effective technique, its use can substantially contribute to the development of future production platforms for recombinant proteins produced in green plant tissues of pharmaceutical and industrial interest. (C) 2011 American Institute of Chemical Engineers Biotechnol. Prog., 27: 1029-1035, 201127410291035Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

High incidence of mixed DNA and RNA virus infections in common bean in Central Brazil.

During the winter crop season in 2016 a very high incidence of viruslike symptoms of mosaic, leaf curling and deformation, and plant dwarfing was reported by farmers in central areas of Brazil. Bean plants were collected in commercial farms in Luziânia, Cristalina and experimental plots in Goiânia and Brasília

Sequência completa do DNA-A de uma nova espécie de Begomovirus infectando tomateiro no Estado de Tocantins, Brasil.

O presente trabalho visou caracterizar uma nova espécie de Begomovirus detectada infectando tomateiro (Solanum lycopersicum L.) no Estado de Tocantins.Resumo 1381. Suplemento. Edição dos resumos do 44º Congresso Brasileiro de Fitopatologia, 2011, Bento Gonçalves

Registro no Estado do Amazonas de um novo begomovírus do tomateiro relacionado com espécies virais da América Central e Caribe.

Esse trabalho visou caracterizar um isolado de Begomovirus encontrado infectando tomateiro no Estado do Amazonas. Plantas com sintomas de clorose interneral e amarelecimento apical foram coletadas na região produtora de tomate para consumo in natura em Silves-AM.Resumo 1382. Suplemento. Edição dos resumos do 44º Congresso Brasileiro de Fitopatologia, 2011, Bento Gonçalves