Variation and evolution of herkogamy in Exochaenium (Gentianaceae): implications for the evolution of distyly

Backgrounds and Aims The spatial separation of stigmas and anthers (herkogamy) in flowering plants functions to reduce self-pollination and avoid interference between pollen dispersal and receipt. Little is known about the evolutionary relationships among the three main forms of herkogamy - approach, reverse and reciprocal herkogamy (distyly) - or about transitions to and from a non-herkogamous condition. This problem was examined in Exochaenium (Gentianaceae), a genus of African herbs that exhibits considerable variation in floral morphology, including the three forms of herkogamy. Methods Using maximum parsimony and maximum likelihood methods, the evolutionary history of herkogamic and non-herkogamic conditions was reconstructed from a molecular phylogeny of 15 species of Exochaenium and four outgroup taxa, based on three chloroplast regions, the nuclear ribosomal internal transcribed spacer (ITS1 and 2) and the 5·8S gene. Ancestral character states were determined and the reconstructions were used to evaluate competing models for the origin of reciprocal herkogamy. Key results Reciprocal herkogamy originated once in Exochaenium from an ancestor with approach herkogamy. Reverse herkogamy and the non-herkogamic condition homostyly were derived from heterostyly. Distylous species possessed pendent, slightly zygomorphic flowers, and the single transition to reverse herkogamy was associated with the hawkmoth pollination syndrome. Reductions in flower size characterized three of four independent transitions from reciprocal herkogamy to homostyly. Conclusions The results support Lloyd and Webb's model in which distyly originated from an ancestor with approach herkogamy. They also demonstrate the lability of sex organ deployment and implicate pollinators, or their absence, as playing an important role in driving transitions among herkogamic and non-herkogamic condition

Influence of stored aqueous ceftriaxone solutions on colony formation by neutrophil and macrophage precursor cells

Aqueous solutions of ceftriaxone in concentrations similar to those achieved in man and stored at temperatures of 4°C, 22°C and 37°C for up to four weeks were tested in methylcellulose cultures of normal bone marrow from ten donors. No significant differences were detected between the colony formation by neutrophil and macrophage precursors in control cultures and in cultures containing stored ceftriaxone. It is concluded that aqueous ceftriaxone solutions stored for up to four weeks at temperatures up to 37°C are not altered to such an extent that they adversely affect colony formation by bone marrow progenitor cells from healthy donor

A new prescription model for regional citrate anticoagulation in therapeutic plasma exchanges.

Regional citrate anticoagulation (RCA) is proposed for various extracorporeal purification techniques to overcome the risk of bleeding that might result from systemic anticoagulation. Yet, no individualized treatment protocol has been proposed for therapeutic plasma exchange (TPE) so far. The objective of this study was to assess the determinants of blood citrate concentration needed and to develop an individualized RCA protocol useful for clinical practice. The study population included 14 patients who underwent a total of 47 TPE sessions. Citrate was infused pre-plasmafilter. Post-plasmafilter and systemic plasma ionized calcium concentrations were measured at standardized time intervals. An algorithm was proposed for the supplementation of calcium. During the discovery phase, citrate was infused at a fixed starting rate, and adapted accordingly to obtained post-plasmafilter ionized calcium levels. Using a mathematical approach, an algorithm was thereafter developed for individualized prescriptions of citrate. Pre-treatment values of hematocrit and plasma ionized calcium were the main determinants of the required rate of citrate infusion. These can be integrated into a final equation enabling to individualize the prescription. A prefilter ionized calcium concentration between 0.24 and 0.33 mmol/l prevented coagulation of the extracorporeal circuit. Significant hypocalcemia occurred in 8.5% of treatments. There were no significant acid-base disturbances. We propose a new protocol, which enables for the first time to individualize the prescription of regional citrate anticoagulation during TPE, in an efficient manner. The immediately obtained regional anticoagulation protects against both the risk of coagulation of the membrane and the exposure to an excess of citrate

Shallow subduction beneath Italy

This paper presents a velocity model of the Italian (central Mediterranean) lithosphere in unprecedented detail. The model is derived by inverting a set of 166,000 Pg and Pn seismic wave arrival times, restricted to the highest-quality data available. The tomographic images reveal the geometry of the subduction-collision system between the European, Adriatic, and Tyrrhenian plates, over a larger volume and with finer resolution than previous studies. We find two arcs of low-Vp anomalies running along the Alps and the Apennines, describing the collision zones of underthrusting continental lithospheres. Our results suggest that in the Apennines, a significant portion of the crust has been subducted below the mountain belt. From the velocity model we can also infer thermal softening of the crustal wedge above the subducting Adriatic plate. In the Tyrrhenian back-arc region, strong and extensive low-Vp anomalies depict upwelling asthenospheric material. The tomographic images also allow us to trace the boundary between the Adriatic and the Tyrrhenian plates at Moho depth, revealing some tears in the Adriatic-Ionian subducting lithosphere. The complex lithospheric structure described by this study is the result of a long evolution; the heterogeneities of continental margins, lithospheric underthrusting, and plate indentation have led to subduction variations, slab tears, and asthenospheric upwelling at the present day. The high-resolution model provided here greatly improves our understanding of the central Mediterranean’s structural puzzle. The results of this study can also shed light on the evolution of other regions experiencing both oceanic and continental subduction

Erratum to: A new prescription model for regional citrate anticoagulation in therapeutic plasma exchanges.

BACKGROUND: Regional citrate anticoagulation (RCA) is proposed for various extracorporeal purification techniques to overcome the risk of bleeding that might result from systemic anticoagulation. Yet, no individualized treatment protocol has been proposed for therapeutic plasma exchange (TPE) so far. The objective of this study was to assess the determinants of blood citrate concentration needed and to develop an individualized RCA protocol useful for clinical practice. METHODS: The study population included 14 patients who underwent a total of 47 TPE sessions. Citrate was infused pre-plasmafilter. Post-plasmafilter and systemic plasma ionized calcium concentrations were measured at standardized time intervals. An algorithm was proposed for the supplementation of calcium. During the discovery phase, citrate was infused at a fixed starting rate, and adapted accordingly to obtained post-plasmafilter ionized calcium levels. Using a mathematical approach, an algorithm was thereafter developed for individualized prescriptions of citrate. RESULTS: Pre-treatment values of hematocrit and plasma ionized calcium were the main determinants of the required rate of citrate infusion. These can be integrated into a final equation enabling to individualize the prescription. A prefilter ionized calcium concentration between 0.24 and 0.33 mmol/l prevented coagulation of the extracorporeal circuit. Significant hypocalcemia occurred in 8.5% of treatments. There were no significant acid–base disturbances. CONCLUSION: We propose a new protocol, which enables for the first time to individualize the prescription of regional citrate anticoagulation during TPE, in an efficient manner. The immediately obtained regional anticoagulation protects against both the risk of coagulation of the membrane and the exposure to an excess of citrate. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12882-017-0494-9) contains supplementary material, which is available to authorized users

Automatic seismic phase picking and consistent observation error assessment: application to Italian seismicity

Accuracy of seismic phase observation and consistency of timing error assessment define the quality of seismic waves arrival times. High-quality and large data sets are prerequisites for seismic tomography to enhance the resolution of crustal and upper mantle structures. In this paperwe present the application of an automated picking system to some 600000 seismograms of local earthquakes routinely recorded and archived by the Italian national seismic network. The system defines an observation weighting scheme calibrated with a hand-picked data subset and mimics the picking by an expert seismologist. The strength of this automatic picking is that once it is tuned for observation quality assessment, consistency of arrival times is strongly improved and errors are independent of the amount of data to be picked. The application to the Italian local seismicity documents that it is possible to automatically compile a precise, homogeneous and large data set of local earthquake Pg and Pn arrivals with related polarities. We demonstrate that such a data set is suitable for high-precision earthquake location, focal mechanism determination and high-resolution seismic tomography