Vitamin K effects in human health: new insights beyond bone and cardiovascular health

Vitamin K is a cofactor for the function of the enzyme \u3b3-glutamyl carboxylase, necessary for the activation of multiple vitamin K dependent-proteins. Vitamin K dependent-proteins (VKDPs) have important roles in bone health, vascular health, metabolism, reproduction as well as in cancer progression. Vitamin K deficiency is common in different conditions, including kidney disease, and it may influence the activity of VKDPs. This review discusses vitamin K status in human health and the physiologic and pathologic roles of VKDPs, beyond the established effects in skeletal and cardiovascular health

Solidification of small para-H2 clusters at zero temperature

We have determined the ground-state energies of para-H$_2$ clusters at zero temperature using the diffusion Monte Carlo method. The liquid or solid character of each cluster is investigated by restricting the phase through the use of proper importance sampling. Our results show inhomogeneous crystallization of clusters, with alternating behavior between liquid and solid phases up to N=55. From there on, all clusters are solid. The ground-state energies in the range N=13--75 are established and the stable phase of each cluster is determined. In spite of the small differences observed between the energy of liquid and solid clusters, the corresponding density profiles are significantly different, feature that can help to solve ambiguities in the determination of the specific phase of H$_2$ clusters.Comment: 17 pages, accepted for publication in J. Phys. Chem.

Search for supersolidity in 4He in low-frequency sound experiments

We present results of the search for supersolid 4He using low-frequency, low-level mechanical excitation of a solid sample grown and cooled at fixed volume. We have observed low frequency non-linear resonances that constitute anomalous features. These features, which appear below about 0.8 K, are absent in 3He. The frequency, the amplitude at which the nonlinearity sets in, and the upper temperature limit of existence of these resonances depend markedly on the sample history.Comment: Submitted to the Quantum Fluids and Solids Conf. Aug. 2006 Kyot

ω-3 Polyunsaturated fatty acids prevent pressure overload-induced ventricular dilation and decrease in mitochondrial enzymes despite no change in adiponectin

<p>Abstract</p> <p>Background</p> <p>Pathological left ventricular (LV) hypertrophy frequently progresses to dilated heart failure with suppressed mitochondrial oxidative capacity. Dietary marine ω-3 polyunsaturated fatty acids (ω-3 PUFA) up-regulate adiponectin and prevent LV dilation in rats subjected to pressure overload. This study 1) assessed the effects of ω-3 PUFA on LV dilation and down-regulation of mitochondrial enzymes in response to pressure overload; and 2) evaluated the role of adiponectin in mediating the effects of ω-3 PUFA in heart.</p> <p>Methods</p> <p>Wild type (WT) and adiponectin-/- mice underwent transverse aortic constriction (TAC) and were fed standard chow ± ω-3 PUFA for 6 weeks. At 6 weeks, echocardiography was performed to assess LV function, mice were terminated, and mitochondrial enzyme activities were evaluated.</p> <p>Results</p> <p>TAC induced similar pathological LV hypertrophy compared to sham mice in both strains on both diets. In WT mice TAC increased LV systolic and diastolic volumes and reduced mitochondrial enzyme activities, which were attenuated by ω-3 PUFA without increasing adiponectin. In contrast, adiponectin-/- mice displayed no increase in LV end diastolic and systolic volumes or decrease in mitochondrial enzymes with TAC, and did not respond to ω-3 PUFA.</p> <p>Conclusion</p> <p>These findings suggest ω-3 PUFA attenuates cardiac pathology in response to pressure overload independent of an elevation in adiponectin.</p

Path integral Monte Carlo simulation of charged particles in traps

This chapter is devoted to the computation of equilibrium (thermodynamic) properties of quantum systems. In particular, we will be interested in the situation where the interaction between particles is so strong that it cannot be treated as a small perturbation. For weakly coupled systems many efficient theoretical and computational techniques do exist. However, for strongly interacting systems such as nonideal gases or plasmas, strongly correlated electrons and so on, perturbation methods fail and alternative approaches are needed. Among them, an extremely successful one is the Monte Carlo (MC) method which we are going to consider in this chapter.Comment: 18 pages, based on talks on Hareaus school on computational methods, Greifswald, September 200

Improved Mitochondrial Function with Diet-Induced Increase in Either Docosahexaenoic Acid or Arachidonic Acid in Membrane Phospholipids

Mitochondria can depolarize and trigger cell death through the opening of the mitochondrial permeability transition pore (MPTP). We recently showed that an increase in the long chain n3 polyunsaturated fatty acids (PUFA) docosahexaenoic acid (DHA; 22:6n3) and depletion of the n6 PUFA arachidonic acid (ARA; 20:4n6) in mitochondrial membranes is associated with a greater Ca2+ load required to induce MPTP opening. Here we manipulated mitochondrial phospholipid composition by supplementing the diet with DHA, ARA or combined DHA+ARA in rats for 10 weeks. There were no effects on cardiac function, or respiration of isolated mitochondria. Analysis of mitochondrial phospholipids showed DHA supplementation increased DHA and displaced ARA in mitochondrial membranes, while supplementation with ARA or DHA+ARA increased ARA and depleted linoleic acid (18:2n6). Phospholipid analysis revealed a similar pattern, particularly in cardiolipin. Tetralinoleoyl cardiolipin was depleted by 80% with ARA or DHA+ARA supplementation, with linoleic acid side chains replaced by ARA. Both the DHA and ARA groups had delayed Ca2+-induced MPTP opening, but the DHA+ARA group was similar to the control diet. In conclusion, alterations in mitochondria membrane phospholipid fatty acid composition caused by dietary DHA or ARA was associated with a greater cumulative Ca2+ load required to induced MPTP opening. Further, high levels of tetralinoleoyl cardiolipin were not essential for normal mitochondrial function if replaced with very-long chain n3 or n6 PUFAs

QTL Mapping in Tropical Maize: II. Comparison of Genomic Regions for Resistance to Diatraea spp.

Southwestern corn borer (SWCB), Diatraea grandiosella (Dyar), and the sugar cane borer (SCB), Diatraea saccharalis (Fabricius), are serious insect pests in maize (Zea mays L.) production areas of Central America and the southern USA. We mapped and characterized quantitative trait loci (QTL) affecting resistance to the leaf feeding generation of SWCB(1 SWCB), compared these QTL with those for resistance to the leaf feeding generation of SCB (1SCB) identified in the same mapping population, and assessed the consistency of QTL for 1SWCB across two populations. One hundred seventy-one F2 genotypes from cross CML131 (susceptible) × CML67 (resistant) and 100 RFLP marker loci were used for the QTL analyses. 1SWCB and 1SCB resistance were assessed in F2:3 lines by leaf damage ratings (LDR) after artificial infestation in field experiments with two replications at one subtropical environment in 2 yr. The method of composite interval mapping (CIM) was used for QTL detection. Estimates of genotypic (σ2g) and genotype × year interaction variance (σ2g) were highly significant for 1SWCB LDR and 1SCB LDR. Phenotypic and genotypic correlations between both traits were 0.62 and 1.02, respectively. for 1SWCB LDR, six QTL were detected explaining 53.3% of σ̂2g, with two QTL displaying significant QTL × year interactions. Ten QTL were detected for 1SCB LDR, accounting for 98.2% of σ̂2g. The QTL showed predominantly additive or partially dominant gene action. Seven out of 10 QTL were pleiotropic to both Diatraea spp. Three genomic regions, on Chromosomes 5 and 9, were consistent with a second mapping population derived from cross Ki3 (susceptible) × CML139 (resistant), for which seven QTL for 1SWCB LDR were found. Marker-assisted ‘gene stacking’ is recommended for transferring pleiotropic QTL into susceptible germplasm and for pyramiding QTL from different sources of insect resistance

Solid 4He and the Supersolid Phase: from Theoretical Speculation to the Discovery of a New State of Matter? A Review of the Past and Present Status of Research

The possibility of a supersolid state of matter, i.e., a crystalline solid exhibiting superfluid properties, first appeared in theoretical studies about forty years ago. After a long period of little interest due to the lack of experimental evidence, it has attracted strong experimental and theoretical attention in the last few years since Kim and Chan (Penn State, USA) reported evidence for nonclassical rotational inertia effects, a typical signature of superfluidity, in samples of solid 4He. Since this "first observation", other experimental groups have observed such effects in the response to the rotation of samples of crystalline helium, and it has become clear that the response of the solid is extremely sensitive to growth conditions, annealing processes, and 3He impurities. A peak in the specific heat in the same range of temperatures has been reported as well as anomalies in the elastic behaviour of solid 4He with a strong resemblance to the phenomena revealed by torsional oscillator experiments. Very recently, the observation of unusual mass transport in hcp solid 4He has also been reported, suggesting superflow. From the theoretical point of view, powerful simulation methods have been used to study solid 4He, but the interpretation of the data is still rather difficult; dealing with the question of supersolidity means that one has to face not only the problem of the coexistence of quantum coherence phenomena and crystalline order, exploring the realm of spontaneous symmetry breaking and quantum field theory, but also the problem of the role of disorder, i.e., how defects, such as vacancies, impurities, dislocations, and grain boundaries, participate in the phase transition mechanism.Comment: Published on J. Phys. Soc. Jpn., Vol.77, No.11, p.11101

Warmer Weather Linked to Tick Attack and Emergence of Severe Rickettsioses

The impact of climate on the vector behaviour of the worldwide dog tick Rhipicephalus sanguineus is a cause of concern. This tick is a vector for life-threatening organisms including Rickettsia rickettsii, the agent of Rocky Mountain spotted fever, R. conorii, the agent of Mediterranean spotted fever, and the ubiquitous emerging pathogen R. massiliae. A focus of spotted fever was investigated in France in May 2007. Blood and tissue samples from two patients were tested. An entomological survey was organised with the study of climatic conditions. An experimental model was designed to test the affinity of Rh. sanguineus for biting humans in variable temperature conditions. Serological and/or molecular tools confirmed that one patient was infected by R. conorii, whereas the other was infected by R. massiliae. Dense populations of Rh. sanguineus were found. They were infected with new genotypes of clonal populations of either R. conorii (24/133; 18%) or R. massiliae (13/133; 10%). April 2007 was the warmest since 1950, with summer-like temperatures. We show herein that the human affinity of Rh. sanguineus was increased in warmer temperatures. In addition to the originality of theses cases (ophthalmic involvements, the second reported case of R. massiliae infection), we provide evidence that this cluster of cases was related to a warming-mediated increase in the aggressiveness of Rh. sanguineus, leading to increased human attacks. From a global perspective, we predict that as a result of globalisation and warming, more pathogens transmitted by the brown dog tick may emerge in the future