278 research outputs found
Cadmium uptake, localization and stress-induced morphogenic response in the fern Pteris vittata.
Cadmium uptake, tissue localization and structural changes induced at cellular level are essential to understand Cd tolerance in plants. In this study we have exposed plants of Pteris vittata to different concentrations of CdCl2 (0, 30, 60, 100 ÎŒM) to evaluate the tolerance of the fern to cadmium. Cadmium content determination and its histochemical localization showed that P. vittata not only takes up, but also transports and accumulates cadmium in the aboveground tissues, delocalizing it mainly in the less bioactive tissues of the frond, the trichomes and the scales. Cadmium tolerance in P. vittata was strictly related to morphogenic response induced by the metal itself in the root system. Adaptive response regarded changes of the root apex size, the developmental pattern of root hairs, the differentiation of xylem elements and endodermal suberin lamellae. All the considered parameters suggest that, in our experimental conditions, 60 ÎŒM of Cd may represent the highest concentration that P. vittata can tolerate; indeed this Cd level even improves the absorbance features of the root and allows good transport and accumulation of the metal in the fronds. The results of this study can provide useful information for phytoremediation strategies of soils contaminated by Cd, exploiting the established ability of P. vittata to transport, delocalize in the aboveground biomass and accumulate polluting metals
Ovatoxin-a, a palytoxin analogue isolated from Ostreopsis cf. ovata Fukuyo: cytotoxic activity and ELISA detection
This study provides the first evaluation of the cytotoxic effects of the recently identified palytoxin (PLTX) analog, ovatoxin-a (OVTX-a), the major toxin produced by Ostreopsis cf. ovata in the Mediterranean Sea. Its increasing detection during Ostreopsis blooms and in seafood highlights the need to characterize its toxic effects and to set up appropriate detection methods. OVTX-a is about 100 fold less potent than PLTX in reducing HaCaT cells
viability (EC50 = 1.1
7 10 129 M vs 1.8
7 10 1211 M, MTT test) in agreement with a reduced binding affinity (Kd = 1.2
7 10 129 vs 2.7
7 10 1211 M, saturation experiments on intact cells). Similarly, OVTX-a hemolytic effect is lower than that of the reference PLTX compound. Ost-D shows the lowest cytotoxicity toward HaCaT keratinocytes, suggesting the lack of a hydroxyl group at C44 as a critical feature for PLTXs cytotoxic effects. A sandwich ELISA developed for PLTX detects also OVTX-a in a sensitive (LOD = 4.2 and LOQ = 5.6 ng/mL) and accurate manner (Bias = 0.3%), also in O. cf. ovata extracts and contaminated mussels. Although in vitro OVTXa appears less toxic than PLTX, its cytotoxicity at nanomolar concentrations after short exposure time rises some concern for human health. The sandwich ELISA can be a viable screening method for OVTXs detection in monitoring program
First Finding of Ostreopsis cf. ovata Toxins in Marine Aerosols
Since the late 1990s, a respiratory syndrome has been repetitively observed in humans concomitant with Ostreopsis spp. blooms (mainly O. cf. ovata) in the Mediterranean area. Previous studies have demonstrated that O. cf. ovata produces analogues of palytoxin (ovatoxins and a putative palytoxin), one of the most potent marine toxins. On the basis of the observed association between O. cf. ovata blooms, respiratory illness in people, and detection of palytoxin complex in algal samples, toxic aerosols, containing Ostreopsis cells and/or the toxins they produce, were postulated to be the cause of human illness. A small scale monitoring study of marine aerosol carried out along the Tuscan coasts (Italy) in 2009 and 2010 is reported.
Aerosols were collected concomitantly with O. cf. ovata blooms, and they were analyzed by both PCR assays and LC-HRMS. The results, besides confirming the presence of O. cf. ovata cells, demonstrated for the first time the occurrence of ovatoxins in the aerosol at levels of 2.4 pg of ovatoxins per liter of air. Given the lack of toxicological data on palytoxins by inhalation exposure, our results are only a first step toward a more comprehensiveunderstanding of the Ostreopsis-related respiratory syndrome
Effects of charge doping on Mott insulator with strong spin-orbit coupling, BaNaOsO
The effects of doping on the electronic evolution of the Mott insulating
state have been extensively studied in efforts to understand mechanisms of
emergent quantum phases of materials. The study of these effects becomes ever
more intriguing in the presence of entanglement between spin and orbital
degrees of freedom. Here, we present a comprehensive investigation of charge
doping in the double perovskite BaNaOsO, a a complex Mott insulator
where such entanglement plays an important role. We establish that the
insulating magnetic ground state evolves from canted antiferromagnet (cAF)to
N\'eel order for dopant levels exceeding ~ 10 %. Furthermore, we determine that
a broken local point symmetry (BLPS) phase, precursor to the magnetically
ordered state, occupies an extended portion of the (H-T) phase diagram with
increased doping. This finding reveals that the breaking of the local cubic
symmetry is driven by a multipolar order, most-likely of the
antiferro-quadrupolar type
Effects of charge doping on Mott insulator with strong spin-orbit coupling, Ba2Na1âxCaxOsO6
The effects of doping on the electronic evolution of the Mott insulating state have been extensively studied in efforts to understand mechanisms of emergent quantum phases of materials. The study of these effects becomes ever more intriguing in the presence of entanglement between spin and orbital degrees of freedom. Here, we present a comprehensive investigation of charge doping in the double perovskite Ba2NaOsO6, a complex Mott insulator where such entanglement plays an important role. We establish that the insulating magnetic ground state evolves from canted antiferromagnet (cAFM) [Lu et al., Nat. Commun. 8, 14407 (2017)] to Neel order for dopant levels exceeding approximate to 10%. Furthermore, we determine that a broken local point symmetry (BLPS) phase, precursor to the magnetically ordered state, occupies an extended portion of the (H-T) phase diagram with increased doping. This finding reveals that the breaking of the local cubic symmetry is driven by a multipolar order, most likely of the antiferro-quadrupolar type [Khaliullin et al., Phys. Rev. Res. 3, 033163 (2021); Churchill and Kee, Phys. Rev. B 105, 014438 (2022)]. Future dynamical measurements will be instrumental in determination of the precise nature of the identified multipolar order
Surgical site infection after caesarean section. Space for post-discharge surveillance improvements and reliable comparisons
Surgical site infections (SSI) after caesarean section (CS) represent a substantial health system concern. Surveying SSI has been associated with a reduction in SSI incidence. We report the findings of three (2008, 2011 and 2013) regional active SSI surveillances after CS in community hospital of the Latium region determining the incidence of SSI. Each CS was surveyed for SSI occurrence by trained staff up to 30 post-operative days, and association of SSI with relevant characteristics was assessed using binomial logistic regression. A total of 3,685 CS were included in the study. A complete 30 day post-operation follow-up was achieved in over 94% of procedures. Overall 145 SSI were observed (3.9% cumulative incidence) of which 131 (90.3%) were superficial and 14 (9.7%) complex (deep or organ/space) SSI; overall 129 SSI (of which 89.9% superficial) were diagnosed post-discharge. Only higher NNIS score was significantly associated with SSI occurrence in the regression analysis. Our work provides the first regional data on CS-associated SSI incidence, highlighting the need for a post-discharge surveillance which should assure 30 days post-operation to not miss data on complex SSI, as well as being less labour intensive
Azimuthal correlation between beauty particles produced in 350 GeV/c -Cu interactions
Using a sample of triggered events, produced in interactions in a copper target, we have identified b\=b events. These include events where the decays of both and are well reconstructed. We measure the azimuthal \hbox{correlation} between beauty particles, and compare our result with predictions based on perturbative QCD
Measurements of charmed-meson production in interactions between 350 GeV/c particles and nuclei
Charmed-meson production by ~GeV/ {} particles incident on copper and tungsten targets has been studied in the WA experiment, performed at the CERN spectrometer. Results obtained are reported and discussed. Reconstruction of decays from the set , , , and charge conjugates has yielded a sample of charmed mesons, produced with \xf > 0, \langle \xf \rangle = 0.18 and \langle {\pt}^2 \rangle = 1.86~{\rm (GeV/}c{\rm )}^2. Assuming a relationship between the cross-section, , per nucleus of mass and the nucleonic cross-section, , the value found for the detected charmed particles is . Taking , the measured cross-sections per nucleon for \xf > 0 production are ~barn for /, ~barn for / and ~barn for /. Differential cross-sections with respect to \xf and {\pt}^2 have been determined for the various types of charmed meson, and particle-antiparticle asymmetries have been analysed
Measurement of the beauty production cross-section in 350 GeV/c -Cu interactions
Using a sample of triggered events, produced in --Cu interactions at 350~GeV, we have identified 26 beauty events. The estimated background in this sample is events. From these data, assuming a linear A-dependence, we measure a beauty production cross-section integrated over all of nb/N
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