High resolution incubation measurements

Soil samples were taken in the bog site of the Siikaneva peat complex and adjacent shrub-belt and forest site in Ruovesi, Finland. Samples were characterized by measuring water content, bulk density, C and N content and further parameters. Samples were collected on 2020-10-22 and 2020-10-23. A subset of samples was taken for a 53 day incubation experiment at 20°C in June 2021. The aim was to measure microbial CO2 production rates of soil samples with varying soil characteristics and water contents to test and establish a new method for incubation measurements with high temporal resolution using a LI-7810. For further information on the method see https://ghg-in-permafrost.awi.de/ . Incubation measurement files contain single incubation measurements of about 10 to 20 minutes length with CO2 concentration measurements with a resolution of 1Hz conducted with a LICOR Trace Gas Analyzer (LI-7810). The data format is described here (section 3, page 5): https://www.licor.com/documents/zsce2z88fgkfpbiguqi0k3g537jllsl7

CO2 fluxes of high resolution incubation measurements

Soil samples were taken in the bog site of the Siikaneva peat complex and adjacent shrub-belt and forest site in Ruovesi, Finland. Samples were characterized by measuring water content, bulk density, C and N content and further parameters. Samples were collected on 2020-10-22 and 2020-10-23. A subset of samples was taken for a 53 day incubation experiment at 20°C in June 2021. The aim was to measure microbial CO2 production rates of soil samples with varying soil characteristics and water contents to test and establish a new method for incubation measurements with high temporal resolution using a LI-7810. For further information on the method see https://ghg-in-permafrost.awi.de/

Physical and chemical characteristics of soil samples

Soil samples were taken in the bog site of the Siikaneva peat complex and adjacent shrub-belt and forest site in Ruovesi, Finland. Samples were characterized by measuring water content, bulk density, C and N content and further parameters. Samples were collected on 2020-10-22 and 2020-10-23. A subset of samples was taken for a 53 day incubation experiment at 20°C in June 2021. The aim was to measure microbial CO2 production rates of soil samples with varying soil characteristics and water contents to test and establish a new method for incubation measurements with high temporal resolution using a LI-7810. For further information on the method see https://ghg-in-permafrost.awi.de/

Identifier of high resolution incubation measurements

Soil samples were taken in the bog site of the Siikaneva peat complex and adjacent shrub-belt and forest site in Ruovesi, Finland. Samples were characterized by measuring water content, bulk density, C and N content and further parameters (see Siikaneva_physicoChemical.csv). Samples collected: 2020-10-22 and 2020-10-23; a subset of samples was taken for a 53 day incubation experiment at 20°C in June 2021. The aim was to measure microbial CO2 production rates of soil samples with varying soil characteristics and water contents to test and establish a new method for incubation measurements with high temporal resolution using a LI-7810. Further information on the method: https://ghg-in-permafrost.awi.de

Quantitative proteomic analysis indicates increased synthesis of a quinolone by Pseudomonas aeruginosa isolates from cystic fibrosis airways

The opportunistic bacterial pathogen Pseudomonas aeruginosa colonizes airways of individuals with cystic fibrosis (CF) with resultant chronic destructive lung disease. P. aeruginosa adaptation to the CF airway includes biofilm formation and antibiotic resistance. Isolates from asymptomatic individuals in the first 3 years of life have unique characteristics, suggesting that adaptation occurs before clinical symptoms. One defined early adaptation is expression of a specific proinflammatory lipopolysaccharide (LPS) that is associated with antimicrobial peptide resistance. This CF-specific LPS is induced when P. aeruginosa is grown in medium that is limited for magnesium. Therefore, qualitative and quantitative proteomic approaches were used to define 1,331 P. aeruginosa proteins, of which 145 were differentially expressed on limitation of magnesium. Among proteins induced by low magnesium were enzymes essential for production of 2-heptyl 3-hydroxy 4-quinolone, the Pseudomonas quinolone signal (PQS), which interacts with the homoserine lactone signaling pathway. Measurement of PQS in P. aeruginosa isolates from asymptomatic children with CF indicated that strains with increased synthesis of PQS are present during early colonization of CF patient airways

Principes de base de la modélisation en transport

info:eu-repo/semantics/publishe