55 research outputs found
Primary Cutaneous Large B-Cell Lymphoma, Leg Type, Localized on the Dorsum
Primary cutaneous large B-cell lymphoma, leg-type (PCLBCL-LT), is a large B-cell lymphoma primarily involving the skin. It is distinguished from the other 3 subsets of this lymphoproliferative disorder by its immunohistopathological features, configuring confluent sheets of medium-sized to large B lymphocytes with round nuclei provided with evident nucleoli, resembling centroblasts or immunoblasts, which express Bcl-6, Bcl-2. Prevalently appearing on the lower limbs, as a single or multicentric and frequently ulcerated skin nodule or plaque, PCLBCL-LT has a worse prognosis than the other large B-cell lymphomas. Moreover, the age of onset is delayed (7th decade) compared to those of the other 3 subtypes (6th decade); it presents a slight female predominance (2:1), and a higher percentage of positivity to Bcl-2. We present a 52-year-old man who showed a 2-year standing, non-ulcerated, round, 4 cm in diameter, red plaque, medially located on the dorsum. After biopsy the diagnosis of PCLBCL-LT was made on histopathological and immunohistochemical studies, the latter showing positivity to CD20, Bcl-2, and Bcl-6. After treatment with radiotherapy the patient has shown a 4.4-year follow-up free of disease
Is the astronomical forcing a reliable and unique pacemaker for climate? A conceptual model study
There is evidence that ice age cycles are paced by astronomical forcing,
suggesting some kind of synchronisation phenomenon. Here, we identify the type
of such synchronisation and explore systematically its uniqueness and
robustness using a simple paleoclimate model akin to the van der Pol relaxation
oscillator and dynamical system theory. As the insolation is quite a complex
quasiperiodic signal involving different frequencies, the traditional concepts
used to define synchronisation to periodic forcing are no longer applicable.
Instead, we explore a different concept of generalised synchronisation in terms
of (coexisting) synchronised solutions for the forced system, their basins of
attraction and instabilities. We propose a clustering technique to compute the
number of synchronised solutions, each of which corresponds to a different
paleoclimate history. In this way, we uncover multistable synchronisation
(reminiscent of phase- or frequency-locking to individual periodic components
of astronomical forcing) at low forcing strength, and monostable or unique
synchronisation at stronger forcing. In the multistable regime, different
initial conditions may lead to different paleoclimate histories. To study their
robustness, we analyse Lyapunov exponents that quantify the rate of convergence
towards each synchronised solution (local stability), and basins of attraction
that indicate critical levels of external perturbations (global stability). We
find that even though synchronised solutions are stable on a long term, there
exist short episodes of desynchronisation where nearby climate trajectories
diverge temporarily (for about 50 kyr). (...)Comment: 22 pages, 18 figure
The restorative role of annexin A1 at the blood–brain barrier
Annexin A1 is a potent anti-inflammatory molecule that has been extensively studied in the peripheral immune
system, but has not as yet been exploited as a therapeutic target/agent. In the last decade, we have undertaken the
study of this molecule in the central nervous system (CNS), focusing particularly on the primary interface between the
peripheral body and CNS: the blood–brain barrier. In this review, we provide an overview of the role of this molecule
in the brain, with a particular emphasis on its functions in the endothelium of the blood–brain barrier, and the protective
actions the molecule may exert in neuroinflammatory, neurovascular and metabolic disease. We focus on the
possible new therapeutic avenues opened up by an increased understanding of the role of annexin A1 in the CNS
vasculature, and its potential for repairing blood–brain barrier damage in disease and aging
Neuroinflammation and Ageing: Current Theories and an Overview of the Data
The increase in the average lifespan and the consequent proportional growth of the elderly segment of society has furthered the interest in studying ageing processes. Ageing may be considered a multifactorial process derived from the interaction between genetic and environmental factors including lifestyle. There is ample evidence in many species that the maximum age attainable (maximum lifespan potential, MLSP) is genetically determined and several mitochondrial DNA polymorphisms are associated with longevity. This review will address the current understanding of the relationship between ageing and several factors both genetics and life style related. Firstly we focused on the most reliable and commonly shared theories which attempt to explain the phenomenon of ageing as the genetic, cellular, neuroendocrine, immunological and free-radicals related theories. Many studies have shown that most of the phenotypic characteristics observed in the aging process are the result of the occurrence, with age, of a low grade chronic pro-inflammatory status called "inflammaging", partially under genetic control. The term indicate that aging is accompanied by a low degree of chronic inflammatory, an up-regulation of inflammatory response and that inflammatory changes are common to many age-related diseases. In this review special attention was dedicated to diseases related to age as atherosclerosis, cancer and Alzheimer disease. Despite the fact that in recent years many theories about ageing have been developed, we are still far from a full understanding of the mechanisms underlying the ageing process
Good Manufacturing Practices-Grade Preformed Ossicular Prostheses From Banked Bone Via Computer Numerically Controlled Micromilling
Objectives: The aim of this study was the fabrication of ossicular replacement prostheses (ORPs) from decellularized
banked cortical bone via computer numerically controlled (CNC) ultraprecision micromilling, in order to obtain preformed
clinical-grade tissue products, reproducing shape, size, and details perfectly comparable to those of synthetic
devices.
Methods: Banked femoral compact bone was used to fabricate partial and total ORPs via CNC micromilling according
to Good Manufacturing Practices procedures. Drawings of ORPs with different shapes and sizes were uploaded to the
computer interface, and different surface-finish parameters were tested. The obtained products underwent dimensional,
weight, and surface characterizations. A histologic analysis was pursued to compare the bone matrix compactness of the
produced ORPs to that of the ear ossicles.
Results: Banked-bone ORPs were produced with high dimensional accuracy. Partial ORP weights averaged (±SD) 31.2
± 0.6 mg, and total ORP weights averaged 69.3 ± 0.7 mg. The best-finish mode allowed microscale or nanoscale roughness
free from machinery textures to be obtained. Finally, the histologic analysis confirmed that the extracellular matrix compactness
of the produced ORPs was suitable for ossicular chain replacement.
Conclusions: This study assesses the fabrication feasibility of novel banked-bone ORPs of extremely high dimensional
accuracy. Such devices are aimed at combining the most favorable aspects of both synthetic (reproducibility, convenience,
and biosafety) and biological replacements (total biocompatibility)
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