13 research outputs found
Predicted sub-populations in a marine shrimp proteome as revealed by combined EST and cDNA data from multiple Penaeus species
Distribution of serotonin and gamma-amino butyric acid immunoreactivity in the deutocerebrum of the male giant freshwater prawn, Macrobrachium rosenbergii
The serotonergic and dopaminergic systems in the Pacific white shrimp, Litopenaeus vannamei: changes in the levels, existence and distribution in the central nervous system and ovary during ovarian maturation cycle
Localization and roles of Corazonin in the giant freshwater prawn, Macrobrachium rosenbergii
Characterization and expression of red pigment concentrating hormone (RPCH) in the female mud crab (Scylla olivacea) and the effect of 5-HT on its expression
Protective activity of medicinal plants and their isolated compounds against the toxic effects from the venom of Naja (cobra) species
Comparison of the adjuvant activity of aluminum hydroxide and calcium phosphate on the antibody response towards Bothrops asper snake venom
The adjuvanticity of aluminum hydroxide and calcium phosphate on the antibody response in
mice towards the venom of the snake Bothrops asper was studied. It was found that, in vitro,
most of the venom proteins are similarly adsorbed by both mineral salts, with the exception
of some basic phospholipases A2, which are better adsorbed by calcium phosphate.
After injection, the adjuvants promoted a slow release of the venom, as judged by the lack
of acute toxicity when lethal doses of venom were administered to mice. Leukocyte recruitment
induced by the venom was enhanced when it was adsorbed on both mineral salts; however,
venom adsorbed on calcium phosphate induced a higher antibody response towards all
tested HPLC fractions of the venom. On the other hand, co-precipitation of venom with
calcium phosphate was the best strategy for increasing: (1) the capacity of the salt to
couple venom proteins in vitro; (2) the venom ability to induce leukocyte recruitment;
(3) phagocytosis by macrophages; and (4) a host antibody response. These findings suggest
that the chemical nature is not the only one determining factor of the adjuvant activity
of mineral salts.International Foundation for Science/[B/5043-1]/IFS/SueciaUniversidad de Costa Rica/[741-B2-501]/UCR/Costa RicaFundação de Amparo à Pesquisa do Estado de São Paulo/[08/57898-0]/INCTTOX-CNPq/FAPESP/BrasilUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP