Aplicación de lixiviados de vermicompost y respuesta agronómica de dos variedades de pimiento

The objective of this study was to evaluate the effect of vermicompost leakage based on Vegetable Residues and Chicken Manure; Vegetable and Agroindustrial Residues, and the combination of the two leakages, in rates of 60 y 90 l ha-1 on the growth and pepper production. The study was carried out in Marconi and Wonder pepper varieties, in total seven treatments were established in three repetitions. The best response in fruit diameter in both evaluated varieties corresponded to the Vegetable and Agroindustrial Residues Leakage 90 l ha-1 and Combined Leakage 90 l ha-1 treatments, being superior to the control with values ​​between 52 and 15%. On the other hand, the longest fruit length was evidenced in the Combined Leakage 90 l ha-1 treatment. Similarly, the root biomass increased in all treatments where leakage was applied.  In production, defined by fruit weight, the outstanding treatments were Vegetable and Agroindustrial Residues Leakage 90 l ha-1 and Combined Leakage 90 l ha-1. Thus, we can conclude that the use of vermicompost leakage in rates of 90 l ha-1 represents an alternative to enhance pepper yield. Furthermore, the combination of these two types of leakage caused a synergistic effect on the physiology and vegetal productionEl objetivo del presente trabajo fué evaluar el efecto de lixiviados de vermicompost a base de Residuos Vegetales y Gallinaza; Residuos Vegetales y Agroindustriales  y la combinación  de los dos lixiviados, en dosis de 60 y 90 l ha-1 sobre el desarrollo y producción del pimiento. El estudió se efectuó en variedades de pimiento  Marconi y Wonder, en total se establecieron siete tratamientos con tres repeticiones. La mejor respuesta en diámetro del fruto en ambas variedades evaluadas correspondió a los tratamientos de Lixiviado de Residuos Vegetales y Agroindustriales 90 l ha-1y Lixiviado Combinado 90 l ha-1 siendo superiores al control con valores de entre 52 y 15%. Por otro lado, la mayor longitud del fruto fue evidenciada en el tratamiento Lixiviado Combinado 90 l ha-1. En tanto que, la biomasa radicular incrementó en todos los tratamientos donde se aplicó el lixiviado. En la producción, definida por peso del fruto, los tratamientos sobresalientes fueron Lixiviado de Residuos Vegetales y Agroindustriales 90 l ha-1 y Lixiviado Combinado 90 l ha-1. De este modo, podemos concluir que el uso de lixiviados de vermicompost en dosis de 90 l ha-1 representa  una alternativa para potenciar el rendimiento del pimiento. En adición, la combinación de estos dos tipos de lixiviados ejerce un efecto sinérgico sobre la fisiología y producción de este vegetal.   &nbsp

Maracuyá: de los recursos genéticos al desarollo tecnológico.

Este libro registra las memorias del 1° Curso Internacional de Pasifloras promovido por Embrapa Cerrados junto a Embrapa Mandioca y Fruticultura en alianza con La Corporación Centro de Desarrollo Tecnológico de las pasifloras de Colombia CDT CEPASS. El curso fue desarrollado en la Ciudad de La Plata, Huila, Colombia con el objetivo de capacitar a productores, técnicos e investigadores en diferentes temas relacionados a la cadena productiva de Pasifloras en los aspectos técnico-científicos, desde la caracterización y uso de los recursos genéticos hasta el desarrollo tecnológico de diferentes especies del género Passiflora.bitstream/item/196061/1/Maracuya.pd

Maracujá: dos recursos genéticos ao desenvolvimento tecnológico.

Este livro registra a memória do 1° Curso Internacional de Passifloras promovido pela Embrapa Cerrados e Embrapa Mandioca e Fruticultura em parceria com a Corporación Centro de Investigación para la Gestión Tecnológica de Passiflora del Departamento del Huila (CEPASS HUILA). O curso foi realizado na cidade de La Plata, Huila, Colômbia com o objetivo de capacitar produtores, técnicos e pesquisadores em diferentes temas ligados à cadeia produtiva das Passifloras e aspectos técnico-científicos desde a caracterização e uso de recursos genéticos até o desenvolvimento tecnológico de diferentes espécies do gênero Passiflora

A homozygous donor splice-site mutation in the meiotic gene MSH4 causes primary ovarian insufficiency

Premature ovarian insufficiency (POI) is a frequent pathology that affects women under 40 years of age, characterized by an early cessation of menses and high FSH levels. Despite recent progresses in molecular diagnosis, the etiology of POI remains idiopathic in most cases. Whole-exome sequencing of members of a Colombian family affected by POI allowed us to identify a novel homozygous donor splice-site mutation in the meiotic gene MSH4 (MutS Homolog 4). The variant followed a strict mendelian segregation within the family and was absent in a cohort of 135 women over 50 years of age without history of infertility, from the same geographical region as the affected family. Exon trapping experiments showed that the splice-site mutation induced skipping of exon 17. At the protein level, the mutation p.Ile743_Lys785del is predicted to lead to the ablation of the highly conserved Walker B motif of the ATP-binding domain, thus inactivating MSH4. Our study describes the first MSH4 mutation associated with POI and increases the number of meiotic/DNA repair genes formally implicated as being responsible for this condition. © The Author 2017. Published by Oxford University Press. All rights reserved

A homozygous donor splice-site mutation in the meiotic gene MSH4 causes primary ovarian insufficiency

Premature ovarian insufficiency (POI) is a frequent pathology that affects women under 40 years of age, characterized by an early cessation of menses and high FSH levels. Despite recent progresses in molecular diagnosis, the etiology of POI remains idiopathic in most cases. Whole-exome sequencing of members of a Colombian family affected by POI allowed us to identify a novel homozygous donor splice-site mutation in the meiotic gene MSH4 (MutS Homolog 4). The variant followed a strict mendelian segregation within the family and was absent in a cohort of 135 women over 50 years of age without history of infertility, from the same geographical region as the affected family. Exon trapping experiments showed that the splice-site mutation induced skipping of exon 17. At the protein level, the mutation p.Ile743_Lys785del is predicted to lead to the ablation of the highly conserved Walker B motif of the ATP-binding domain, thus inactivating MSH4. Our study describes the first MSH4 mutation associated with POI and increases the number of meiotic/DNA repair genes formally implicated as being responsible for this condition. © The Author 2017. Published by Oxford University Press. All rights reserved

Nueva etiología genética de LVNC descubierta por un método de filtrado combinatorio de datos de secuenciación de exoma completo original

Background: Left ventricular non-compaction (LVNC) is a cardiomyopathy characterized by deep intra-trabecular recesses and prominent left ventricular trabeculae. This cardiomyopathy has remarkable phenotypic and genetic heterogeneity. Its molecular diagnosis via next generation sequencing is challenging due to the large amount of potential aetiological variants, especially in complex cases originating from mutations in various genes. Objectives: This study sought to determine a novel diagnostic approach for identifying the molecular cause of LVNC in patients showing intra-familial phenotype variability and evoking a digenic aetiology. Methods: We performed whole-exome sequencing (WES) in a three-generation family of patients affected by different degrees of LVNC. An enriched subset of 94 LVNC candidate genes was analysed in silico using an original WES data combinatory filtering method. Results: We identified two novel heterozygous mutations, one in ACTC1 (c.740G>A; p.Gly247Asp) and another in ITGA7 (c.3280C>T; p.Gln1094Ter) which perfectly segregated with the phenotype. These variants underlined the disease’s digenic origin, explaining intra-familial phenotype variability. We unambiguously determined that ITGA7 mutations lead to LVNC. Conclusions: The results argues in favour of the final common hypothesis proposed as being involved in complex heterogeneous cardiovascular diseases. The genomic/computational approach presented here is an advantageous and efficient method for dissecting the molecular basis of LVNC’s origin and we strongly recommend it for diagnostic/prognostic purposes. We have described the direct association of an ITGA7 mutation and LVNC pathophysiology for the first time, thereby enriching the repertoire of genes to be systematically analysed in LVNC patients