Investigating dune-building feedback at the plant level: Insights from a multispecies field experiment

Coastal foredunes provide the first line of defense against rising sea levels and storm surge and for this reason there is increasing interest in understanding and modeling foredune formation and post-storm recovery. However, there is limited observational data available to provide empirical guidance for the development of model parameterizations. To provide guidance for improved representation of dune grass growth in models, we conducted a two-year multi-species transplant experiment on Hog Island, VA, U.S.A. and measured the dependence of plant growth on elevation and distance from the shoreline, as well as the relationship between plant growth and sand accumulation. We tracked total leaf growth (length) and aboveground leaf length and found that Ammophila breviligulata (American beachgrass) and Uniola paniculata (sea oats) grew more than Spartina patens (saltmeadow cordgrass) by a factor of 15% (though not statistically significant) and 45%, respectively. Our results also suggest a range of basal/frontal area ratios (an important model parameter) from 0.5-1 and a strong correlation between transplant growth and total sand deposition for all species at the scale of two years, but not over shorter temporal scales. Distance from the shoreline and elevation had no effect on transplant growth rate but did have an effect on survival. Based on transplant survival, the seaward limit of vegetation at the end of the experiment was approximately 30 m from the MHWL and at an elevation of 1.43 m, corresponding to inundation less than 7.5% of the time according to total water level calculations. Results from this experiment provide evidence for the dune-building capacity of all three species, suggesting S. patens is not a maintainer species, as previously thought, but rather a moderate dune builder even though its growth is less stimulated by sand deposition than A. breviligulata and U. paniculata

Specific Interactions of Clausin, a New Lantibiotic, with Lipid Precursors of the Bacterial Cell Wall

We investigated the specificity of interaction of a new type A lantibiotic, clausin, isolated from Bacillus clausii, with lipid intermediates of bacterial envelope biosynthesis pathways. Isothermal calorimetry and steady-state fluorescence anisotropy (with dansylated derivatives) identified peptidoglycan lipids I and II, embedded in dodecylphosphocholine micelles, as potential targets. Complex formation with dissociation constants of ∼0.3 μM and stoichiometry of ∼2:1 peptides/lipid intermediate was observed. The interaction is enthalpy-driven. For the first time, to our knowledge, we evidenced the interaction between a lantibiotic and C55-PP-GlcNAc, a lipid intermediate in the biosynthesis of other bacterial cell wall polymers, including teichoic acids. The pyrophosphate moiety of these lipid intermediates was crucial for the interaction because a strong binding with undecaprenyl pyrophosphate, accounting for 80% of the free energy of binding, was observed. No binding occurred with the undecaprenyl phosphate derivative. The pentapeptide and the N-acetylated sugar moieties strengthened the interaction, but their contributions were weaker than that of the pyrophosphate group. The lantibiotic decreased the mobility of the pentapeptide. Clausin did not interact with the water-soluble UDP-MurNAc- and pyrophosphoryl-MurNAc-pentapeptides, pointing out the importance of the hydrocarbon chain of the lipid target