Identification and Genome-Wide Prediction of DNA Binding Specificities for the ApiAP2 Family of Regulators from the Malaria Parasite

The molecular mechanisms underlying transcriptional regulation in apicomplexan parasites remain poorly understood. Recently, the Apicomplexan AP2 (ApiAP2) family of DNA binding proteins was identified as a major class of transcriptional regulators that are found across all Apicomplexa. To gain insight into the regulatory role of these proteins in the malaria parasite, we have comprehensively surveyed the DNA-binding specificities of all 27 members of the ApiAP2 protein family from Plasmodium falciparum revealing unique binding preferences for the majority of these DNA binding proteins. In addition to high affinity primary motif interactions, we also observe interactions with secondary motifs. The ability of a number of ApiAP2 proteins to bind multiple, distinct motifs significantly increases the potential complexity of the transcriptional regulatory networks governed by the ApiAP2 family. Using these newly identified sequence motifs, we infer the trans-factors associated with previously reported plasmodial cis-elements and provide evidence that ApiAP2 proteins modulate key regulatory decisions at all stages of parasite development. Our results offer a detailed view of ApiAP2 DNA binding specificity and take the first step toward inferring comprehensive gene regulatory networks for P. falciparum

Intégrer la gestion des ressources en eau et l'aménagement du territoire dans les espaces périphériques : une approche par l'économie politique

International audienceInstitutional obstacles to integrated water management at the river basin scale have been discussed in detail in the water governance literature, but there has been less attention to the development of analytical framework for understanding local government cooperation. In this study, a median voter model was developed to describe the political processes by which municipalities lend their support to land-use control in river basin management planning. Relative income, population growth, and land cover data — as measured at municipality level — are advanced to be the main determinants of municipalities׳ positioning on additional environmental zoning. The consultation process for the SAGE (planning and water management scheme) for the Gironde estuary and associated areas was used as a case study. Spatial logit estimation of the determinants of the results of voting in this consultation process suggests that municipalities׳ decisions are strongly influenced by the landscape preferences of the median voter. Acceptance of the SAGE project is an increasing function of relative income and population growth, as measured at municipality level. Furthermore, the municipalities that reject it are mainly those with the largest agricultural areas. The results confirm the existence of a very strong political component in the process by which a municipality decides whether or not to support a river basin plan. This decision can be linked to the preservation of natural landscape amenities in peripheral areas, while elsewhere it is connected to the protection of farming. Theoretical and empirical developments of political economy analysis provide an alternative framework by which to understand institutional fits and interplay in water resource managemen

Synthesis, characterisation and aqueous behaviour of a one-ended perfluorocarbon-modified poly(ethylene glycol)

International audienceEstablishing structure–properties relationships for an associative polymer requires a precise knowledge of its structure. In previous works, we studied water-soluble telechelic perfluorocarbon (C8F17) derivatives of poly(ethylene glycol)s. They exhibit stronger hydrophobic intermolecular associations than the corresponding hydrocarbon derivatives (C8H17). We now report the synthesis and study of one-ended perfluorocarbon derivative of poly(ethylene glycol). The composition and structure of this polymer were elucidated before analysing its behaviour in aqueous solution by viscosimetry and 19F NMR. The synthesis procedure allows us to reach total grafting. This polymer presents a micellar behaviour above 2×10−4 g/ml and an associative behaviour above 10−3 g/ml

Approximation, synthese et realisation de filtres microondes a cavites bimodes, a reponses pseudo-elliptiques dissymetriques

Available at INIST (FR), Document Supply Service, under shelf-number : 9888 C, issue : a.1990 n.124 / INIST-CNRS - Institut de l'Information Scientifique et TechniqueSIGLEFRFranc

Configurations organisationnelles et gouvernance des infrastructures - Enseignements pour la gestion patrimoniale des infrastructures

The article aims at shedding light on the governance of infrastructures by characterizing the organizational configurations and their evolution. On the basis of an analysis crossing different types of infrastructures (drinking water and sewerage networks, hydraulic structures, road structures (bridges, tunnels) and minor road network), we identify the factors explaining the observed organizational configurations and their evolution (public policies, specificities of territories and actors involved). The analysis allows for drawing some insights with regard to the patrimonial management of infrastructures.Optimiser le renouvellement et les performances des ouvrages et réseaux qui maillent le territoire reste un enjeu majeur pour une gestion patrimoniale des infrastructures en pleine évolution. À travers l'analyse transversale de l'organisation des acteurs impliqués dans la gestion de différents types d'infrastructures - réseaux d'eau potable et d'assainissement, ouvrages hydrauliques, voiries locales et ouvrages d'art - l'article permet ici de mettre en évidence la grande diversité des configurations existantes et leurs implications en termes de gestion patrimoniale