Computer-based attention-demanding testing unveils severe neglect in apparently intact patients

We tested a group of ten post-acute right-hemisphere damaged patients. Patients had no neglect according to paper-and-pencil cancellation tasks. They were administered computer-based single- and dual-tasks, requiring to orally name the position of appearance (e.g. left vs. right) of briefly-presented lateralized targets. Patients omitted a consistent number of contralesional targets (approximate to 40%) under the single-task condition. When required to perform a concurrent task which recruited additional attentional resources (dual-tasks), patients' awareness for contralesional hemispace was severely affected, with less than one third of contralesional targets detected (approximate to 70% of omissions). In contrast, performance for ipsilesional (right-sided) targets was close to ceiling, showing that the deficit unveiled by computer-based testing selectively affected the contralesional hemispace. We conclude that computer-based, attention-demanding tasks are strikingly more sensitive than cancellation tasks in detecting neglect, because they are relatively immune to compensatory strategies that are often deployed by post-acute patients

Chromosome compaction and chromatin stiffness enhance diffusive loop extrusion by slip-link proteins

We use Brownian dynamics simulations to study the formation of chromatin loops through diffusive sliding of slip-link-like proteins, mimicking the behaviour of cohesin molecules. We recently proposed that diffusive sliding is sufficient to explain the extrusion of chromatin loops of hundreds of kilo-base-pairs (kbp), which may then be stabilised by interactions between cohesin and CTCF proteins. Here we show that the flexibility of the chromatin fibre strongly affects this dynamical process, and find that diffusive loop extrusion is more efficient on stiffer chromatin regions. We also show that the dynamics of loop formation are faster in confined and collapsed chromatin conformations but that this enhancement is counteracted by the increased crowding. We provide a simple theoretical argument explaining why stiffness and collapsed conformations favour diffusive extrusion. In light of the heterogeneous physical and conformational properties of eukaryotic chromatin, we suggest that our results are relevant to understand the looping and organisation of interphase chromosomes in vivo

Effect of different diets on the development, mortality, survival, food uptake and fecundity of Tupiocoris cucurbitaceus (Hemiptera: Miridae)

Several Miridae (Hemiptera) species have been identified as useful predators for biological control of the greenhouse whitefly, Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae). There is interest in determining the effects of different diets on Tupiocoris cucurbitaceus (Spinola) (Hemiptera: Miridae) in order to facilitate their breeding and use in biological control. Given that mirids can be both phytophagous and zoophytophagous, the developmental time, mortality, survival, feeding and fecundity of this species on tomato and tobacco leaves with and without the addition of T. vaporariorum or Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) eggs were studied. To determine embryonic duration, T. cucurbitaceus was allowed to oviposit on tobacco plants for 24 h at 26 °C, 83% RH and a photoperiod of 16:8 h L:D. The time of onset of the first instar nymphs was recorded. Subsequently, these nymphs fed on different diets on 9 cm × 1.5 cm plates under the same environmental conditions. Results showed that diet influences the duration of development in T. cucurbitaceus, with nymphal stadia being shorter and the adult stadium being longer when fed prey than when not fed prey. With the addition of prey, nymphal mortality was generally lower and adult survival was higher. The high consumption of T. vaporariorum eggs by T. cucurbitaceus suggests the need for subsequent studies on the latter for inclusion of this species in biological control programs in greenhouses

Predictions for surveys with the SPICA Mid-infrared Instrument

We present predictions for number counts and redshift distributions of galaxies detectable in continuum and in emission lines with the Mid-infrared (MIR) Instrument (SMI) proposed for the Space Infrared Telescope for Cosmology and Astrophysics. We have considered 24 MIR fine-structure lines, four polycyclic aromatic hydrocarbon bands (at 6.2, 7.7, 8.6 and 11.3 mu m) and two silicate bands (in emission and in absorption) at 9.7 and 18.0 mu m. Six of these lines are primarily associated with active galactic nuclei (AGNs), the others with star formation. A survey with the SMI spectrometers of 1-h integration per field of view (FoV) over an area of 1 deg(2) will yield 5 sigma detections of similar or equal to 140 AGN lines and of similar or equal to 5.2 x 10(4) star-forming galaxies, similar or equal to 1.6 x 10(4) of which will be detected in at least two lines. The combination of a shallow (20.0 deg(2), 1.4 x 10(-1) h integration per FoV) and a deep survey (6.9 x 10(-3) deg(2), 635 h integration time), with the SMI camera, for a total of similar to 1000 h, will accurately determine the MIR number counts of galaxies and of AGNs over five orders of magnitude in flux density, reaching values more than one order of magnitude fainter than the deepest Spitzer 24 mu m surveys. This will allow us to determine the cosmic star formation rate (SFR) function down to SFRs more than 100 times fainter than reached by the Herschel Observatory

Otimização do método de extração de DNA de Magnaporthe oryzae de trigo.

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Meiotic Arrest Compromises Pollen Fertility in an Interspecific Hybrid Between \u3cem\u3eBrachiaria Ruziziensis\u3c/em\u3e X \u3cem\u3eBrachiaria Decumbens\u3c/em\u3e (Gramineae)

Disruptions in meiosis, development of the free microspores, microspore mitosis, pollen differentiation or anthesis can result in male-sterile plants (Glover et al., 1998). An understanding of the meiotic process is pivotal to work on reproduction, fertility, genetics and breeding in plants, with serious implications in crop production (Armstrong & Jones, 2003). Some African species of Brachiaria are the most important for pastures in the American tropics due to good adaptation and production. Artificial hybridization is underway in Embrapa to improve production, quality and insect resistance (Valle & Miles, 2001). For a cultivar to be successfully adopted good seed production and pollen viability are required. This paper reports on meiotic abnormalities impairing pollen fertility in a hybrid between B. ruziziensis x B. decumbens