Nuxcell Neo® improves vaccine efficacy in antibody response

Current vaccination protocols raise concerns about the efficacy of immunization. There is evidence that changes in the gut microbiota can impact immune response. The formation of the gut microbiota in newborns plays a crucial role in immunity. Probiotic bacteria and prebiotics present important health-promoting and immunomodulatory properties. Thus, we hypothesize that pro and prebiotic supplementation can improve the efficacy of vaccination in newborns. In this protocol, newborn mice were used and treated with a single-dose rabies vaccine combined with Nuxcell Neo® (2 g/animal/week) for 3 weeks. Samples were collected on days 7, 14, and 21 after vaccination for analysis of cytokines and concentration of circulating antibodies. Our results show an increased concentration of antibodies in animals vaccinated against rabies and simultaneously treated with Nuxcell Neo® on days 14 and 21 when compared to the group receiving only the vaccine. In the cytokine levels analysis, it was possible to observe that there weren't relevant and significant changes between the groups, which demonstrates that the health of the animal remains stable. The results of our study confirm the promising impact of the use of Nuxcell Neo® on the immune response after vaccination

Preparation and characterization of 4-nitrochalcone-folic acid-poly(methyl methacrylate) nanocapsules and cytotoxic activity on HeLa and NIH3T3 cells

Chalcones of natural origin are plant metabolites which have been explored because of the cytotoxic effects towards tumor cells. In this study, the synthetic chalcone 4-nitrochalcone (4NC) and its encapsulated form in folic acid-poly(methyl methacrylate) (PMMA) nanocapsules (4NC-FA-PMMA) were investigated towards the cytotoxic effects on erytrocytes, mouse embryonic fibroblasts cells (NIH3T3), and tumor cells (HeLa cells). Characterization of 4NC-FA-PMMA presented spherical morphology with nanocapsule-type structure, a mean size of 170 ± 6 nm, a negative zeta potential of (−40 ± 4 mV), and an entrapment efficiency of ~80%. In HeLa cells, 4NC induced a dose-dependent reduction in cell viability, with an IC value of 46.7 μM. The cytotoxicity was confirmed by morphological alterations, cell death, and an increase in the population of hypodiploid cells. When 4NC-FA-PMMA nanocapsules were employed at concentrations of 15 and 30 μM the reduction in cell viability was higher than that of 4NC. In addition, 4NC and 4NC-FA-PMMA nanocapsules did not present any cytotoxic effect on the NIH3T3 cells and human erythrocytes up to 50 μM. These results demonstrated that the 4NC encapsulation in PMMA nanocapsules with folic acid-modified surface is a better system to promote selective cytotoxic effects to HeLa cells. Therefore, this formulation could be considered a promising preparation with potential chemotherapeutic action

Resumos concluídos - Ciências Farmacêuticas

Resumos concluídos - Ciências Farmacêutica

Resumos em andamento - Neurociências

Resumos em andamento - Neurociência

Resumos em andamento - Neurociências

Resumos em andamento - Neurociência

Resumos concluídos - Bioquímica

Resumos concluídos - Bioquímic