Recovery of protein, chitin, carotenoids and glycosaminoglycans from Pacific white shrimp (Litopenaeus vannamei) processing waste

Shrimp head waste is a major byproduct of crustacean processing in North-eastern Brazil and represents an interesting source of bioactive molecules. Additionally, its use increases the sustainability of processing fishery products. the present study reports a process developed for recovering bioactive molecules from shrimp heads through autolysis. A protein hydrolysate (120 +/- 0.4 g) formed by a 9% (w/v) solution was recovered and lyophilized from I kg of shrimp heads. Approximately 195 +/- 0.5 mg of carotenoids was recovered as an ethanolic extract. the recovery of chitin and chitosan were 25 +/- 2 g kg(-1) and 17 +/- 4 g kg(-1) wet processing waste, respectively. Chitosans were characterized by C-13 NMR, and FT-IR analysis and exhibited a variable degree of deacetylation (60-80%). Sulfated glycosaminoglycans that exhibited electrophoretic migration similar to mammalian standards were also recovered (79 +/- 2 mg kg(-1) wet processing waste), and their degradation products suggested the presence of C6-sulfated heparan sulfate. These data point to the feasibility of an integrated process for isolating highly bioactive molecules, such as sulfated- and amino-polysaccharides, with a broad spectrum of applications from shrimp processing waste. (C) 2011 Elsevier B.V. All rights reserved.MPAConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FINEPEMBRAPAUFPEFACEPEUniv Fed Pernambuco, Lab Imunopatol Keizo Asami, Dept Bioquim, Lab Enzimol LABENZ, BR-50670420 Recife, PE, BrazilUniversidade Federal de São Paulo, INFAR, Lab Biol Mol, BR-04044020 São Paulo, BrazilUniv Fed Rio Grande do Norte, Ctr Biociencias, Dept Bioquim, BR-59072000 Natal, RN, BrazilUniversidade Federal de São Paulo, INFAR, Lab Biol Mol, BR-04044020 São Paulo, BrazilWeb of Scienc