Crossing relationships of <i>Culex pipiens</i> isofemale lines according to <i>w</i>Pip groups.

<p>Total indicates the total number of reciprocal crosses performed to established CI patterns, and N the number of crosses that were compatible (C), uni-directionally incompatible (UIC) and bi-directionally incompatible (BIC). SD = standard deviation. In incompatible crosses, HR  = 0%; in compatible crosses, HR >90%. For more details about crosses within <i>w</i>Pip groups see Tables S2, S3, S4, S5 and S6 in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087336#pone.0087336.s001" target="_blank">File S1</a> whilst for crosses between <i>w</i>Pip groups see Tables S7, S8, S9 and S10 in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087336#pone.0087336.s001" target="_blank">File S1</a>.</p

Schematic representation of the crossing relationships between <i>Culex pipiens</i> lines infected with different <i>w</i>Pip groups.

<p>Numbers indicates the number of reciprocal crosses analyzed. In all compatible crosses, hatching rate (HR) >90% and in incompatible crosses, HR  = 0%.</p

Crossing relationships between isofemale lines infected with strains from the <i>w</i>Pip-I group and from different geographic origins.

<p>Crosses were classified either compatible (C, hatching rate (HR) >90%) or incompatible (IC, HR  = 0%, in bold). The number of egg rafts collected in each cross is bracketed. Boxed crosses were performed between mosquito lines from the same population. *, Crosses corresponding to data from Atyame et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087336#pone.0087336-Atyame1" target="_blank">[17]</a>. Note that crosses between mosquitoes from the same isofemale line are always compatible.</p

Assessment of mating competitiveness of LR[<i>w</i>Pip(Is)] males under semi-field conditions.

<p>In each confrontation, 200 field females were mixed with each of the four following field to LR[<i>w</i>Pip(Is)] males ratios: 1:0 (200 field males), 1:1 (200 field males and 200 LR[<i>w</i>Pip(Is)] males), 1:5 (200 field males and 1000 LR[<i>w</i>Pip(Is)] males) and 0:1 (200 LR[<i>w</i>Pip(Is)] males). The trials of the 1:0 and 0:1 ratios were performed in April; two trials for the 1:1 ratio were performed in April and four in December; whilst for the 1:5 ratio two trials were performed in April and three in December. Expected frequency of infertile egg rafts (in black) was calculated assuming equal competitiveness of LR[<i>w</i>Pip(Is)] and field males. Total embryonic mortality (HR = 0%) was noted in all infertile egg rafts.</p

Complete data

Complete dat

Survival curves of field males (N = 220; dotted line) and LR[<i>w</i>Pip(Is)] males (N = 177; solid line) in the semi-field setup.

<p>Survival curves of field males (N = 220; dotted line) and LR[<i>w</i>Pip(Is)] males (N = 177; solid line) in the semi-field setup.</p

R model for the invasion dynamics of homozygous-sublethal (HS) D alleles (cf. Fig. 4)

R model for the invasion dynamics of homozygous-sublethal (HS) D alleles (cf. Fig. 4

ace-1 sequences Intron2 - partial Exon3 (cf. Fig2)

ace-1 sequences Intron2 - partial Exon3 (cf. Fig2

Wing size of males.

<p>LR = wild type males, LRi = wild type irradiated males and AR<i>w</i>P_LR = incompatible males. N = number of mosquitoes measured. a and b represent statistical group (LRT).</p