Towards a codification of practical knowledge

International audienceAs practical knowledge seems to have a central place in organisational issues, we focus on possibilities to study and formalize it. From an unusual theoretical perspective, we view practical knowledge as embodied knowing which only is only manifest through action in a particular situation. Although this knowledge is largely implicit, we try to make what is 'articulable' explicit. After highlighting the stakes involved in the codification of practices, we review the ontological and epistemological assumptions underlying the method developed. It is founded on participant observation, a video recording of a situated subjective perspective and an ex post interview using this perspective to aid an actor to make part of his/her practical knowledge explicit. We present its implementation within research on polar expeditions in order to understand how an experienced actor deals with risks. In conclusion, we point out (1) the importance of this kind of data in knowledge management, (2) some lines of further research

Multiply attenuated, self-inactivating lentiviral vectors efficiently transduce human coronary artery cells in vitro and rat arteries in vivo.

Endothelial cells (ECs) in normal vessels are poorly transducible by retroviral vectors, which require cell division for gene transduction. Among retroviruses, lentiviruses have the unique ability to integrate their genome into the chromatin of nondividing cells. Here we show that multiply attenuated, self-inactivating, lentiviral vectors transduce both proliferating and growth-arrested human umbilical vein ECs (HUVECs), human coronary artery ECs (HCAECs), and human coronary artery smooth muscle cells (HCASMCs), with high efficacy. Lentiviral vectors containing the enhanced green fluorescence protein (EGFP) transgene driven by either the cytomegalovirus or the elongation factor-1alpha promoter, but not the phosphoglycerate kinase promoter, directed high-level EGFP expression in endothelial and smooth muscle cells. The endothelium-specific Tie2 promoter also directed transgene expression in ECs. Re-insertion of cis-acting sequences from pol of human immunodeficiency virus type 1 (HIV-1) into the vectors improved transgene expression. A lentiviral vector containing the vascular endothelial growth factor transgene promoted EC proliferation and sprouting in vitro. In vivo gene transfer was studied by lumenal infusion of vector containing solutions into rat carotid arteries. Lentivirus-mediated EGFP gene transfer was observed in approximately 5% of ECs. Lentiviral vectors containing the LacZ transgene achieved detectable beta-galactosidase activity in rat arteries, albeit at a lower level compared with adenoviral vectors. This difference was mainly due to the lower concentration of lentiviral vector preparations. Lentivirus-mediated gene transfer was associated with minimal neointimal hyperplasia and scant inflammatory cell infiltrates in the media and adventitia. These observations indicate that lentiviral vectors may be useful for genetic modifications of vascular cells in vitro and in vivo

Crisis communication during the Ebola outbreak in West Africa: The paradoxes of decontextualized contextualization

As organizations involved in the 2014–2016 Ebola virus disease (EVD) outbreak response in West Africa are now drawing lessons from the crisis, the “manufacture of consent” (Burawoy 1979) emerges as an important issue. Recommendations and public health interventions developed during the response were met with suspicion and often resistances by affected populations, pushing involved organizations and actors to reflect about the validity of their risk communication tools and concepts. These difficulties stressed the numerous shortcomings of risk communication practices, which proved inefficient in an unfamiliar social and cultural context. Many reasons can be pointed-out to explain this failure to communicate risks and public health measures effectively under these circumstances. They include: unrealistic goals for communication; lack of integration of social science skills and knowledge in communication guidelines and human resources; underestimation of the breadth of communication-related tasks; over-segmentation and lack of clarity of communication concepts and expertise (risk communication, crisis communication, social mobilization, and health promotion are all but a few of these categories). Among all these possible lines of inquiry, I want to address what can arguably be considered the most fundamental flaw of crisis communication during the West African EVD episode: its inability to take into account and analyze efficiently the context of the intervention