8 research outputs found
Identification of latexin by a proteomic analysis in rat normal articular cartilage
<p>Abstract</p> <p>Background</p> <p>Osteoarthritis (OA) is characterized by degeneration of articular cartilage. Animal models of OA induced are a widely used tool in the study of the pathogenesis of disease. Several proteomic techniques for selective extraction of proteins have provided protein profiles of chondrocytes and secretory patterns in normal and osteoarthritic cartilage, including the discovery of new and promising biomarkers. In this proteomic analysis to study several proteins from rat normal articular cartilage, two-dimensional electrophoresis and mass spectrometry (MS) were used. Interestingly, latexin (LXN) was found. Using an immunohistochemical technique, it was possible to determine its localization within the chondrocytes from normal and osteoarthritic articular cartilage.</p> <p>Results</p> <p>In this study, 147 proteins were visualized, and 47 proteins were identified by MS. A significant proportion of proteins are involved in metabolic processes and energy (32%), as well as participating in different biological functions including structural organization (19%), signal transduction and molecular signaling (11%), redox homeostasis (9%), transcription and protein synthesis (6%), and transport (6%). The identified proteins were assigned to one or more subcellular compartments.</p> <p>Among the identified proteins, we found some proteins already recognized in other studies such as OA-associated proteins. Interestingly, we identified LXN, an inhibitor of mammalian carboxypeptidases, which had not been described in articular cartilage. Immunolabeling assays for LXN showed a granular distribution pattern in the cytoplasm of most chondrocytes of the middle, deep and calcified zones of normal articular cartilage as well as in subchondral bone. In osteoarthritic cartilage, LXN was observed in superficial and deep zones.</p> <p>Conclusions</p> <p>This study provides the first proteomic analysis of normal articular cartilage of rat. We identified LXN, whose location was demonstrated by immunolabeling in the chondrocytes from the middle, deep and calcified zones of normal articular cartilage, and superficial and deep zones of osteoarthritic cartilage.</p
The orosomucoid 1 protein (α1 acid glycoprotein) is overexpressed in odontogenic myxoma
Odontogenic myxoma (OM) is a benign, but locally invasive, neoplasm occurring in the jaws. However, the molecules implicated in its development are unknown. OM as well as Dental Follicle (DF), an odontogenic tissue surrounding the enamel organ, is derived from ectomesenchymal/mesencyhmal elements. To identify some protein that could participate in the development of this neoplasm, total proteins from OM were
separated by two-dimensional electrophoresis and the profiles were compared with those obtained from DF, used
as a control
Proteomic analysis of rat cartilage: the identification of differentially expressed proteins in the early stages of osteoarthritis
Applied genomic in cerebrovascular disease
Las enfermedades cerebrovasculars implican las alteraciones causadas por el proceso patolĂłgico de los vasos sanguĂneos, que afectan a una o varias áreas del cerebro. La enfermedad cerebro-vascular tambiĂ©n se conoce como ictus o ictus; Es la tercera causa de muerte en todo el mundo y es la patologĂa neurolĂłgica con mayor tasa de prevalencia. La enfermedad cerebrovascular induce varios cambios en la expresiĂłn genĂ©tica dentro de la unidad neurovascular (cĂ©lulas gliales, neuronas y cĂ©lulas ependimales); Principalmente, los cambios en el estrĂ©s oxidativo y la entrada de calcio en las cĂ©lulas, podrĂan iniciar la muerte celular y la destrucciĂłn del tejido, causando una lesiĂłn irreversible en el cerebro, perdiendo varias funciones. La lesiĂłn hace que la activaciĂłn de las vĂas de señalizaciĂłn responda al estrĂ©s, donde muchas molĂ©culas, como las proteĂnas y el ARNm, actĂşan como intermediarios para activar o desactivar los mecanismos de estrĂ©s; estas molĂ©culas son capaces de transmitir señales extracelulares en el nĂşcleo activando la expresiĂłn gĂ©nica temprana como protooncogenes y varios factores de transcripciĂłn para reparar la lesiĂłn cerebral. Es importante conocer la relaciĂłn de los cambios en la expresiĂłn genĂ©tica y las proteĂnas para evitar el desarrollo de lesiones y activar la recuperaciĂłn del cerebro. Este conocimiento nos permite diagnosticar la tasa de lesiones y proponer mecanismos terapĂ©uticos para reducir o evitar los efectos adversos a tiempo, antes de que comience la muerte celular
The orosomucoid 1 protein (α1 acid glycoprotein) is overexpressed in odontogenic myxoma
Abstract Background Odontogenic myxoma (OM) is a benign, but locally invasive, neoplasm occurring in the jaws. However, the molecules implicated in its development are unknown. OM as well as Dental Follicle (DF), an odontogenic tissue surrounding the enamel organ, is derived from ectomesenchymal/mesencyhmal elements. To identify some protein that could participate in the development of this neoplasm, total proteins from OM were separated by two-dimensional electrophoresis and the profiles were compared with those obtained from DF, used as a control. Results We identified eight proteins with differential expression; two of them were downregulated and six upregulated in OM. A spot consistently overexpressed in odontogenic myxoma, with a molecular weight of 44-kDa and a pI of 3.5 was identified as the orosomucoid 1 protein. Western blot experiments confirmed the overexpression of this protein in odontogenic myxoma and immunohistochemical assays showed that this protein was mainly located in the cytoplasm of stellate and spindle-shaped cells of this neoplasm. Conclusion Orosomucoid 1, which belongs to a group of acute-phase proteins, may play a role in the modulation of the immune system and possibly it influences the development of OM.</p
Reporte PAP Servicios de AtenciĂłn ClĂnica Interdisciplinaria primavera 2022
El Proyecto de AplicaciĂłn Profesional AtenciĂłn ClĂnica Interdisciplinaria tiene sus orĂgenes en el PAP que desde hace más de 10 años está vinculado con el DIF Zapopan y que en un principio estaba más fundamentado en una base psicoanalĂtica.
Actualmente, el proyecto se ofrece desde el Departamento de PsicologĂa, EducaciĂłn y Salud del ITESO con el objetivo de ofrecer servicios de atenciĂłn y orientaciĂłn profesional a los usuarios que la solicitan para encontrar respuesta a los diferentes
motivos de consulta psicolĂłgica y nutricional que sean referidos. Como parte del contexto actual del COVID-19 y del retorno a la presencialidad que se ha suscitado desde agosto del 2021, el proyecto ha contribuido a ofrecer un espacio de atenciĂłn
en modalidad hĂbrida (presencial y en lĂnea) a la salud integral de los usuarios de los diferentes escenarios. La concepciĂłn de interdisciplina atañe principalmente a las licenciaturas de PsicologĂa, NutriciĂłn y Derecho para que de manera coordinada se
ofrezcan servicios que logren hacer un buen diagnóstico, planeación e intervención en problemáticas tales como la violencia de género, el maltrato infantil, la depresión, la ansiedad y el desarrollo de una vida saludable en los aspectos nutricionales,
emocionales y de acceso a la justicia. En el presente documento se presentan los avances más significativos de los servicios de atenciĂłn clĂnica que se ofrecen, asĂ como de los productos que para cada escenario el proyecto desarrollĂł durante el
periodo de primavera de 2022.ITESO, A.C