1,182 research outputs found
Oscillatory tunneling magnetoresistance in magnetic tunnel junctions with inserted nonmagnetic layer
Oscillatory tunneling magnetoresistance (TMR) as a function of spacer
thickness is investigated theoretically for a magnetic tunnel junction with a
nonmagnetic layer inserted between the tunnel barrier and the ferromagnetic
layer. TMR is characterized in an analytical form, that is expressed with the
transmission and reflection amplitudes of single interfaces at the Fermi level,
and by the extremal wavevectors. Electronic structures with multiple bands are
taken into account in the derivation characterizing the TMR, and the proposed
analytical expression can be directly applied to real junctions. Based on our
model, the features of TMR dependence on spacer thickness are discussed,
including selection rules for the oscillation period. Numerical calculations
are performed using an envelope-function theory for several cases, and we show
that our model is in good agreement with the exact result.Comment: 21 pages (preprint), 6 figure
Preparative Synthesis of dTDP-L-Rhamnose Through Combined Enzymatic Pathways
dTDP-L-rhamnose, an important precursor of O-antigen, was prepared on a large scale from dTMP by executing an one-pot reaction in which six enzymes are involved. Two enzymes, dTDP-4-keto-6-deoxy-D-glucose 3,5-epimerase and dTDP-4-keto-rhamnose reductase, responsible for the conversion of dTDP-4-keto-6-deoxy- D-glucose to dTDP-L-rhamnose, were isolated from their putative sequences in the genome of Mesorhizobium loti, functionally expressed in Escherichia coli, and their enzymatic activities were identified. The two enzymes were combined with an enzymatic process for dTDP-4- keto-6-deoxy-D-glucose involving TMP kinase, acetate kinase, dTDP-glucose synthase, and dTDP-glucose 4,6- dehydratase, which allowed us to achieve a preparative scale synthesis of dTDP-L-rhamnose using dTMP and glucose-1-phosphate as starting materials. About 82% yield of dTDP-L-rhamnose was obtained based on initial dTMP concentration at 20 mM dTMP, 1 mM ATP, 10 mM NADH, 60 mM acetyl phosphate, and 80 mM glucose-1- phosphate. From the reaction with 20 ml volume, approximately 180 mg of dTDP-L-rhamnose was obtained in an overall yield of 60% after two-step purification, that is, anion exchange chromatography and gel filtration for desalting. The purified product was identifiedbyHPLC, ESI-MS,andNMR,showingabout95%purity
Quantum advantage through the magic pentagram problem
Through the two specific problems, the 2D hidden linear function problem and
the 1D magic square problem, Bravyi et al. have recently shown that there
exists a separation between and , where
and are the classes of polynomial-size and
constant-depth quantum and classical circuits with bounded fan-in gates,
respectively. In this paper, we present another problem with the same property,
the magic pentagram problem based on the magic pentagram game, which is a
nonlocal game. In other words, we show that the problem can be solved with
certainty by a circuit but not by any
circuits.Comment: 10 pages, 5 figure
One-pot Enzymatic Synthesis of Deoxy-thymidine-diphosphate (TDP)-2-deoxy-∝-d-glucose Using Phosphomannomutase
Production of deoxy-thymidine-diphosphate (TDP)-sugars as substrates of glycosyltransferases, has been one of main hurdles for combinatorial antibiotic biosynthesis, which combines sugar moiety with aglycon of various antibiotics. Here, we report the one-pot enzymatic synthesis of TDP-2-deoxy-glucose employing high efficient TMP kinase (TMK; E.C. 2.7.2.12), acetate kinase (ACK; E.C. 2.7.1.21), and TDP-glucose synthase (TGS; E.C. 2.7.7.24) with phosphomannomutase (PMM; E.C. 5.4.2.8). In this study, replacing phosphoglucomutase (PGM; E.C. 5.4.2) by PMM from Escherichia coli gave four times higher specific activity on 2-deoxy-6-phosphate glucose, suggesting that the activity on 2-deoxy-glucose-6-phosphate was mainly affected by PMM activity, not PGM activity. Using an in vitro system starting from TMP and 2-deoxy-glucose-6-phosphate glucose, TDP-2-deoxy-glucose (63% yield) was successfully synthesized. Considering low productivity of NDP-sugars from cheap starting materials, this paper showed how production of NDP-sugars could be enhanced by controlling mutase activity
The Intestinal Copper Exporter CUA-1 Is Required for Systemic Copper Homeostasis in Caenorhabditis elegans
Copper plays key catalytic and regulatory roles in biochemical processes essential for normal growth, development, and health. Defects in copper metabolism cause Menkes and Wilson’s disease, myeloneuropathy, and cardiovascular disease and are associated with other pathophysiological states. Consequently, it is critical to understand the mechanisms by which organisms control the acquisition, distribution, and utilization of copper. The intestinal enterocyte is a key regulatory point for copper absorption into the body; however, the mechanisms by which intestinal cells transport copper to maintain organismal copper homeostasis are poorly understood. Here, we identify a mechanism by which organismal copper homeostasis is maintained by intestinal copper exporter trafficking that is coordinated with extraintestinal copper levels in Caenorhabditis elegans. Specifically, we show that CUA-1, the C. elegans homolog of ATP7A/B, localizes to lysosome-like organelles (gut granules) in the intestine under copper overload conditions for copper detoxification, whereas copper deficiency results in a redistribution of CUA-1 to basolateral membranes for copper efflux to peripheral tissues. Worms defective in gut granule biogenesis exhibit defects in copper sequestration and increased susceptibility to toxic copper levels. Interestingly, however, a splice isoform CUA-1.2 that lacks a portion of the N-terminal domain is targeted constitutively to the basolateral membrane irrespective of dietary copper concentration. Our studies establish that CUA-1 is a key intestinal copper exporter and that its trafficking is regulated to maintain systemic copper homeostasis. C. elegans could therefore be exploited as a whole-animal model system to study regulation of intra- and intercellular copper trafficking pathways
Strain-gradient-induced magnetic anisotropy in straight-stripe mixed-phase bismuth ferrites: An insight into flexomagnetic phenomenon
Implementation of antiferromagnetic compounds as active elements in
spintronics has been hindered by their insensitive nature against external
perturbations which causes difficulties in switching among different
antiferromagnetic spin configurations. Electrically-controllable strain
gradient can become a key parameter to tune the antiferromagnetic states of
multiferroic materials. We have discovered a correlation between an
electrically-written straight-stripe mixed-phase boundary and an in-plane
antiferromagnetic spin axis in highly-elongated La-5%-doped BiFeO thin
films by performing polarization-dependent photoemission electron microscopy in
conjunction with cluster model calculations. Model Hamiltonian calculation for
the single-ion anisotropy including the spin-orbit interaction has been
performed to figure out the physical origin of the link between the strain
gradient present in the mixed phase area and its antiferromagnetic spin axis.
Our findings enable estimation of the strain-gradient-induced magnetic
anisotropy energy per Fe ion at around 510 eV m, and provide a
new pathway towards an electric-field-induced 90 rotation of
antiferromagnetic spin axis at room temperature by flexomagnetism.Comment: 32 pages, 5 figure
The Analysis of Antecedents for the Video Telephony Service Adoption: From the Value-Based Perspective
Korean Telecommunications Industry has a large scale market and boasts on high service quality and high technologies enough to provide the Video Telephony Service (VTS) satisfactorily. For many years, Korean telephone companies have been investing enormous sums to advertise their services widely and to allow their customers to change their cell phones for the third-generation (3G) devices indispensable for the service. However, despite their efforts, the VTS adoption rate in Korea is very low and it seems that customers seldom feel the necessity to use. From this viewpoint, it becomes necessary to find the antecedents influencing the intention to use for the VTS empirically. For this purpose, we proposed several hypotheses from the perspective of the Value-based Adoption Model (VAM). VAM is a conceptual model suggested to overcome some limitations of the Technology Acceptance Model (TAM) in explaining the adoption of new Information and Communication Technology (ICT) such as Mobile Internet where customers play the role of service consumer rather than simply technology users. We conducted a survey on 125 samples and found that customers perceive the value of VTS when they can recognize the service is functionally useful (Perceived Usefulness) and when they feel they can put themselves forward by using it (Self-Expression). On the other hand, the other factors including Technical Complexity, Privacy Concern and Perceived Price (Fee) don’t have statistically significant influences on the Perceived Value of VTS
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