6 research outputs found
Genomeâwide CRISPR screen identifies ZIC2 as an essential gene that controls the cell fate of early mesodermal precursors to human heart progenitors
Cardiac progenitor formation is one of the earliest committed steps of human cardiogenesis
and requires the cooperation of multiple gene sets governed by developmental
signaling cascades. To determine the key regulators for cardiac progenitor
formation, we have developed a two-stage genome-wide CRISPR-knockout screen.
We mimicked the progenitor formation process by differentiating human pluripotent
stem cells (hPSCs) into cardiomyocytes, monitored by two distinct stage markers of
early cardiac mesodermal formation and commitment to a multipotent heart progenitor
cell fate: MESP1 and ISL1, respectively. From the screen output, we compiled a
list of 15 candidate genes. After validating seven of them, we identified ZIC2 as an
essential gene for cardiac progenitor formation. ZIC2 is known as a master regulator
of neurogenesis. hPSCs with ZIC2 mutated still express pluripotency markers. However,
their ability to differentiate into cardiomyocytes was greatly attenuated. RNASeq
profiling of the ZIC2-mutant cells revealed that the mutants switched their cell
fate alternatively to the noncardiac cell lineage. Further, single cell RNA-seq analysis
showed the ZIC2 mutants affected the apelin receptor-related signaling pathway during
mesoderm formation. Our results provide a new link between ZIC2 and human
cardiogenesis and document the potential power of a genome-wide unbiased
CRISPR-knockout screen to identify the key steps in human mesoderm precursor
cell- and heart progenitor cell-fate determination during in vitro hPSC cardiogenesis.Swedish Research Council for Health, Working Life and Welfare (Forte)Knut and Alice Wallenberg Foundation, KAW 2013.0028Swedish Research Council, 541-2013-8351, 539â2013â7002European Research Council Advanced Research Grant Award, AdG743225Publishe
Population and single-cell analysis of human cardiogenesis reveals unique LGR5 ventricular progenitors in embryonic outflow tract.
The morphogenetic process of mammalian cardiac development is complex and highly regulated spatiotemporally by multipotent cardiac stem/progenitor cells (CPCs). Mouse studies have been informative for understanding mammalian cardiogenesis; however, similar insights have been poorly established in humans. Here, we report comprehensive gene expression profiles of human cardiac derivatives from multipotent CPCs to intermediates and mature cardiac cells by population and single-cell RNA-seq using human embryonic stem cell-derived and embryonic/fetal heart-derived cardiac cells micro-dissected from specific heart compartments. Importantly, we discover a uniquely human subset of cono-ventricular region-specific CPCs, marked by LGR5. At 4 to 5 weeks of fetal age, the LGR5+ population appears to emerge specifically in the proximal outflow tract of human embryonic hearts and thereafter promotes cardiac development and alignment through expansion of the ISL1+TNNT2+ intermediates. The current study contributes to a deeper understanding of human cardiogenesis, which may uncover the putative origins of certain human congenital cardiac malformations.The Knut and Alice Wallenberg Foundation (KAW Dnr 2013.0028)Swedish Research Council Distinguished Professor Grant Dnr 541-2013-8351AstraZeneca PharmaceuticalsKarolinska InstitutetSwedish Heart Lung Foundation No. 20150421EMBO long-term fellowship (ALTF 620-2014)European Research Council Advanced Research Grant Award (AdG743225)Publishe
Human ISL1+ ventricular progenitors self-assemble into an in vivo functional heart patch and preserve cardiac function post infarction
The generation of human pluripotent stem cell (hPSC)-derived ventricular progenitors and their assembly into a 3-dimensional in vivo functional ventricular heart patch has remained an elusive goal. Herein, we report the generation of an enriched pool of hPSC-derived ventricular progenitors (HVPs), which can expand, differentiate, self-assemble, and mature into a functional ventricular patch in vivo without the aid of any gel or matrix. We documented a specific temporal window, in which the HVPs will engraft in vivo. On day 6 of differentiation, HVPs were enriched by depleting cells positive for pluripotency marker TRA-1-60 with magnetic-activated cell sorting (MACS), and 3 million sorted cells were sub-capsularly transplanted onto kidneys of NSG mice where, after 2 months, they formed a 7 mm x 3 mm x 4 mm myocardial patch resembling the ventricular wall. The graft acquired several features of maturation: expression of ventricular marker (MLC2v), desmosomes, appearance of T-tubule-like structures, and electrophysiological action potential signature consistent with maturation, all this in a non-cardiac environment. We further demonstrated that HVPs transplanted into un-injured hearts of NSG mice remain viable for up to 8 months. Moreover, transplantation of 2 million HVPs largely preserved myocardial contractile function following myocardial infarction. Taken together, our study reaffirms the promising idea of using progenitor cells for regenerative therapy.ERC AdG743225Swedish Research Council Distinguished Professor Grant Dnr 541-2013-8351The Knut and Alice Wallenberg Foundation (KAW Dnr 2013.0028)Horizon 2020 research and innovation programme grant agreement No 647714Publishe
Human ISL1+ ventricular progenitors self-assemble into an in vivo functional heart patch and preserve cardiac function post infarction
The generation of human pluripotent stem cell (hPSC)-derived ventricular progenitors and their assembly into a 3-dimensional in vivo functional ventricular heart patch has remained an elusive goal. Herein, we report the generation of an enriched pool of hPSC-derived ventricular progenitors (HVPs), which can expand, differentiate, self-assemble, and mature into a functional ventricular patch in vivo without the aid of any gel or matrix. We documented a specific temporal window, in which the HVPs will engraft in vivo. On day 6 of differentiation, HVPs were enriched by depleting cells positive for pluripotency marker TRA-1-60 with magnetic-activated cell sorting (MACS), and 3 million sorted cells were sub-capsularly transplanted onto kidneys of NSG mice where, after 2 months, they formed a 7 mm Ă 3 mm Ă 4 mm myocardial patch resembling the ventricular wall. The graft acquired several features of maturation: expression of ventricular marker (MLC2v), desmosomes, appearance of T-tubule-like structures, and electrophysiological action potential signature consistent with maturation, all this in a non-cardiac environment. We further demonstrated that HVPs transplanted into un-injured hearts of NSG mice remain viable for up to 8 months. Moreover, transplantation of 2 million HVPs largely preserved myocardial contractile function following myocardial infarction. Taken together, our study reaffirms the promising idea of using progenitor cells for regenerative therapy