Extended Sentinel Monitoring of Helicoverpa zea Resistance to Cry and Vip3Aa Toxins in Bt Sweet Corn: Assessing Changes in Phenotypic and Allele Frequencies of Resistance

Transgenic corn and cotton that produce Cry and Vip3Aa toxins derived from Bacillus thuringiensis (Bt) are widely planted in the United States to control lepidopteran pests. The sustainability of these Bt crops is threatened because the corn earworm/bollworm, Helicoverpa zea (Boddie), is evolving a resistance to these toxins. Using Bt sweet corn as a sentinel plant to monitor the evolution of resistance, collaborators established 146 trials in twenty-five states and five Canadian provinces during 2020–2022. The study evaluated overall changes in the phenotypic frequency of resistance (the ratio of larval densities in Bt ears relative to densities in non-Bt ears) in H. zea populations and the range of resistance allele frequencies for Cry1Ab and Vip3Aa. The results revealed a widespread resistance to Cry1Ab, Cry2Ab2, and Cry1A.105 Cry toxins, with higher numbers of larvae surviving in Bt ears than in non-Bt ears at many trial locations. Depending on assumptions about the inheritance of resistance, allele frequencies for Cry1Ab ranged from 0.465 (dominant resistance) to 0.995 (recessive resistance). Although Vip3Aa provided high control efficacy against H. zea, the results show a notable increase in ear damage and a number of surviving older larvae, particularly at southern locations. Assuming recessive resistance, the estimated resistance allele frequencies for Vip3Aa ranged from 0.115 in the Gulf states to 0.032 at more northern locations. These findings indicate that better resistance management practices are urgently needed to sustain efficacy the of corn and cotton that produce Vip3Aa

Single and sequential applications of dicamba for the control of glyphosate-resistant common ragweed in glyphosate- and dicamba-resistant soybean

Confirmation of glyphosate-resistant (GR) weeds in southwestern Ontario has led to a change in weed management practices, particularly in soybean. Registered soil applied herbicides have been identified that have activity on GR common ragweed, however, due to the long emergence period of common ragweed, additional postemergence options are required. The recent development of glyphosate- and dicamba-resistant soybean (Roundup Ready Xtend soybean) allows for the preplant and postemergence application of dicamba. Three field studies were conducted in Ontario, Canada in a field with confirmed GR common ragweed. GR common ragweed interference resulted in 75% yield loss in soybean compared to the weed-free check. At 4 weeks after application, dicamba tank-mixed with glyphosate, applied preplant only, postemergence only, or preplant followed by postemergence controlled GR common ragweed up to 94, 87 and 99%, respectively. The availability of dicamba for use in glyphosate- and dicamba-resistant soybean will provide an additional mode of action for weed management in soybean.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author