1,586 research outputs found
Naar een werkzame uitvoeringsorganisatie van de sociale zekerheid
Towards a new organisational structure for the provision of social security:Samenvatting in het engels. The large demand for social security provisions in The Netherlands can partly be ascribed to the complicated and fragmentedorganisational structure for the payments of benefits and the public employment offices. This article discusses a blueprint for a more comprehensiveorganisation, where in each region one office has both the responsibility for the payment of benefits and for public employment provision. The regionaloffices obtain their budget from the government and are allowed to supplement these budgets with the proceeds of work done by recipients of benefits.The incentives for the regional offices to (re)employ as many recipients of benefits as possible may lead to a decrease in the demand for socialsecurity and hence enhance labour participation in The Netherlands
On the extent and role of the small proteome in the parasitic eukaryote Trypanosoma brucei
Background: Although technical advances in genomics and proteomics research have yielded a better understanding of the coding capacity of a genome, one major challenge remaining is the identification of all expressed proteins, especially those less than 100 amino acids in length. Such information can be particularly relevant to human pathogens, such as Trypanosoma brucei, the causative agent of African trypanosomiasis, since it will provide further insight into the parasite biology and life cycle. Results: Starting with 993 T. brucei transcripts, previously shown by RNA-Sequencing not to coincide with annotated coding sequences (CDS), homology searches revealed that 173 predicted short open reading frames in these transcripts are conserved across kinetoplastids with 13 also conserved in representative eukaryotes. Mining mass spectrometry data sets revealed 42 transcripts encoding at least one matching peptide. RNAi-induced down-regulation of these 42 transcripts revealed seven to be essential in insect-form trypanosomes with two also required for the bloodstream life cycle stage. To validate the specificity of the RNAi results, each lethal phenotype was rescued by co-expressing an RNAi-resistant construct of each corresponding CDS. These previously non-annotated essential small proteins localized to a variety of cell compartments, including the cell surface, mitochondria, nucleus and cytoplasm, inferring the diverse biological roles they are likely to play in T. brucei. We also provide evidence that one of these small proteins is required for replicating the kinetoplast (mitochondrial) DNA. Conclusions: Our studies highlight the presence and significance of small proteins in a protist and expose potential new targets to block the survival of trypanosomes in the insect vector and/or the mammalian host
Quantitative mass spectrometry and PAR-CLIP to identify RNA-protein interactions
Systematic analysis of the RNA-protein interactome requires robust and scalable methods. We here show the combination of two completely orthogonal, generic techniques to identify RNA-protein interactions: PAR-CLIP reveals a collection of RNAs bound to a protein whereas SILAC-based RNA pull-downs identify a group of proteins bound to an RNA. We investigated binding sites for five different proteins (IGF2BP1-3, QKI and PUM2) exhibiting different binding patterns. We report near perfect agreement between the two approaches. Nevertheless, they are non-redundant, and ideally complement each other to map the RNA-protein interaction network
Six-dimensional Supergravity and Projective Superfields
We propose a superspace formulation of N=(1,0) conformal supergravity in six
dimensions. The corresponding superspace constraints are invariant under
super-Weyl transformations generated by a real scalar parameter. The known
variant Weyl super-multiplet is recovered by coupling the geometry to a
super-3-form tensor multiplet. Isotwistor variables are introduced and used to
define projective superfields. We formulate a locally supersymmetric and
super-Weyl invariant action principle in projective superspace. Some families
of dynamical supergravity-matter systems are presented.Comment: 39 pages; v3: some modifications in section 2; equations (2.3),
(2.14b), (2.16) and (2.17) correcte
Dipole Interactions and Electrical Polarity in Nanosystems -- the Clausius-Mossotti and Related Models
Point polarizable molecules at fixed spatial positions have solvable
electrostatic properties in classical approximation, the most familiar being
the Clausius-Mossotti (CM) formula. This paper generalizes the model and
imagines various applications to nanosystems. The behavior is worked out for a
sequence of octahedral fragments of simple cubic crystals, and the crossover to
the bulk CM law is found. Some relations to fixed moment systems are discussed
and exploited. The one-dimensional dipole stack is introduced as an important
model system. The energy of interaction of parallel stacks is worked out, and
clarifies the diverse behavior found in different crystal structures. It also
suggests patterns of self-organization which polar molecules in solution might
adopt. A sum rule on the stack interaction is found and tested. Stability of
polarized states under thermal fluctuations is discussed, using the
one-dimensional domain wall as an example. Possible structures for polar hard
ellipsoids are considered. An idea is formulated for enhancing polarity of
nanosystems by intentionally adding metallic coatings.Comment: 18 pages (includes 6 embedded figures and 3 tables). New references,
and other small improvements. Scheduled for publication by J. Chem. Phys.,
Jan. 200
Groene groei: hoe bereiken we dat?
Het regeerakkoord van het kabinet Rutte II belooft veel aandacht voor economische groei en duurzaamheid. Dit artikel bespreekt op welke wijze inhoud kan worden gegeven aan de wens van het kabinet om economische groei en zorg voor het milieu met elkaar te combineren. Vanuit het perspectief van de economische welvaartsanalyse gaat het daarbij om de afweging tussen de materiele welvaart en de milieukwaliteit. Vanuit de economische groeitheorie wordt geschetst welke mogelijkheden er zijn voor een dergelijke groene groei. De verschillende manieren om de productiviteit te beïnvloeden en te bevorderen vormen daarbij het aangrijpingspunt voor het beleid. Een verhoging van de milieu efficiëntie, bijvoorbeeld door een milieubesparende technologie kan tot groei met minder milieugebruik leiden, maar dat hoeft niet altijd het geval te zijn
The Real Anatomy of Complex Linear Superfields
Recent work on classicication of off-shell representations of N-extended
worldline supersymmetry without central charges has uncovered an unexpectedly
vast number--trillions of even just (chromo)topology types--of so called
adinkraic supermultiplets. Herein, we show by explicit analysis that a
long-known but rarely used representation, the complex linear supermultiplet,
is not adinkraic, cannot be decomposed locally, but may be reduced by means of
a Wess-Zumino type gauge. This then indicates that the already unexpectedly
vast number of adinkraic off-shell supersymmetry representations is but the
proverbial tip of the iceberg.Comment: 21 pages, 4 figure
Extended supersymmetric sigma models in AdS_4 from projective superspace
There exist two superspace approaches to describe N=2 supersymmetric
nonlinear sigma models in four-dimensional anti-de Sitter (AdS_4) space: (i) in
terms of N=1 AdS chiral superfields, as developed in arXiv:1105.3111 and
arXiv:1108.5290; and (ii) in terms of N=2 polar supermultiplets using the AdS
projective-superspace techniques developed in arXiv:0807.3368. The virtue of
the approach (i) is that it makes manifest the geometric properties of the N=2
supersymmetric sigma-models in AdS_4. The target space must be a non-compact
hyperkahler manifold endowed with a Killing vector field which generates an
SO(2) group of rotations on the two-sphere of complex structures. The power of
the approach (ii) is that it allows us, in principle, to generate hyperkahler
metrics as well as to address the problem of deformations of such metrics.
Here we show how to relate the formulation (ii) to (i) by integrating out an
infinite number of N=1 AdS auxiliary superfields and performing a superfield
duality transformation. We also develop a novel description of the most general
N=2 supersymmetric nonlinear sigma-model in AdS_4 in terms of chiral
superfields on three-dimensional N=2 flat superspace without central charge.
This superspace naturally originates from a conformally flat realization for
the four-dimensional N=2 AdS superspace that makes use of Poincare coordinates
for AdS_4. This novel formulation allows us to uncover several interesting
geometric results.Comment: 88 pages; v3: typos corrected, version published in JHE
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