63 research outputs found
Influência de fatores ambientais na distribuição de famílias de insetos aquáticos em rios no sul do Brasil
Identification of genes involved in gentamicin-induced nephrotoxicity in rats – A toxicogenomic investigation
1G99 : AN ANCIENT ENZYME: ACETATE KINASE FROM METHANOSARCINA THERMOPHILA
Experimental Technique/Method:X-RAY DIFFRACTION Resolution:2.5 Classification:TRANSFERASE Release Date:2000-12-27 Deposition Date:2000-11-22 Revision Date:2008-04-27#2011-07-13#2018-01-31 Molecular Weight:89834.51 Macromolecule Type:Protein Residue Count:816 Atom Site Count:6120 DOI:10.2210/pdb1g99/pdb Abstract: Acetate kinase, an enzyme widely distributed in the Bacteria and Archaea domains, catalyzes the phosphorylation of acetate. We have determined the three-dimensional structure of Methanosarcina thermophila acetate kinase bound to ADP through crystallography. As we previously predicted, acetate kinase contains a core fold that is topologically identical to that of the ADP-binding domains of glycerol kinase, hexokinase, the 70-kDa heat shock cognate (Hsc70), and actin. Numerous charged active-site residues are conserved within acetate kinases, but few are conserved within the phosphotransferase superfamily. The identity of the points of insertion of polypeptide segments into the core fold of the superfamily members indicates that the insertions existed in the common ancestor of the phosphotransferases. Another remarkable shared feature is the unusual, epsilon conformation of the residue that directly precedes a conserved glycine residue (Gly-331 in acetate kinase) that binds the alpha-phosphate of ADP. Structural, biochemical, and geochemical considerations indicate that an acetate kinase may be the ancestral enzyme of the ASKHA (acetate and sugar kinases/Hsc70/actin) superfamily of phosphotransferases
Early Reactions to Frustration: Developmental Trends in Anger, Individual Response Styles, and Caregiving Risk Implications in Infancy
Factors Influencing Maternal Behavioral Adaptability: Maternal Depressive Symptoms and Child Negative Affect
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