Caries Management: A Journey between Black’s principals and Minimally Invasive Concepts

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Bonding of Resin-modified glass ionomer-based adhesive to dentine

This journal suppl. entitled: Special Issue: Abstracts of the 2012 FDI Annual World Dental CongressTheme: Preventive dentistry - Public Health (Oral Presentation)OBJECTIVES: To compare the effect of conditioning dentine with either 37% phosphoric acid for 5 s or 10% polyacrylic acid (PAA) for 10 s on microtensile bond strength (lTBS) of resin-modified glass ionomer based-adhesive to dentine and micro-morphology of the bonded interface. METHODS: Twenty sound perma...postprin

Shear Bond Strength and Remineralisation Effect of a Casein Phosphopeptide-Amorphous Calcium Phosphate-Modified Glass Ionomer Cement on Artificial “Caries-Affected” Dentine

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Mechanical properties, water sorption characteristics, and compound release of grape seed extract-incorporated resins

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Anti-collagenolytic activity of proanthocyanidin

Theme: Dental Treatment and Restorative Dentistry - Materials (Oral Presentation)This journal suppl. entitled: Special Issue: Abstracts of the 2012 FDI Annual World Dental CongressBACKGROUND: Resin-dentine bond has been shown to be less durable due to collagen degradation within hybrid layer by matrix metalloprotenases (MMPs) and cysteine cathepsins. OBJECTIVE: This study evaluated the anti-protease effect of proanthocyanidin (PA) on soluble and matrix-bound MMPs and cysteine cathep...postprin

Contract Management Process Maturity:' Analysis of Recent Organizational Assessments

Symposium Presentation (for Acquisition Research Program)Symposium PresentationNaval Postgraduate School Acquisition Research ProgramApproved for public release; distribution is unlimited

Pits and fissures: Relative space contribution in fissures from sealants, prophylaxis pastes and organic remnants

The document attached has been archived with permission from the Australian Dental Association. An external link to the publisher’s copy is included.Background: Previous studies by the authors have looked at the nature of the fissure system of human permanent molars and premolars, and has provided evidence for the presence of a prismless layer of enamel. It was noted during these studies that the fissure spaces were often occupied by material other than the fissure sealant. The aim of this study was to define these materials and to look at the percentage contribution of each to the sealed fissure space. MethodS: A sample of teeth, both molars and premolars, were sealed with an unfilled fissure sealant after prophylaxis with a coloured prophylaxis paste. In one group, the crown of the tooth was removed by dissolution in hydrochloric acid following placement of the sealant. This revealed a negative image of the fissure system and its contents. The second group of teeth was sectioned following sealing, and the contents of the fissure space were analyzed. Results: The negative image of the fissure system displayed the fissure contents by colour and the sectioned teeth were able to be computer analyzed to establish the relative contribution of sealant, prophylaxis paste and organic material to the fissure space. Conclusions: Sealant contribution was in the range of 14- 96 per cent, prophylaxis paste from 0-50 per cent and organic remnants 0-55 per cent. The presence of these last two components could contribute to sealant loss

Prevention of secondary caries using silver diamine fluoride treatment and casein phosphopeptide-amorphous calcium phosphate modified glass-ionomer cement

Objective: To study the effect of silver diamine fluoride (SDF) treatment and incorporating casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) into a glass-ionomer cement (GIC) to prevent secondary caries. Method: A cervical cavity was prepared on 32 premolars for the following restoration groups: group 1, conventional GIC restoration; group 2, SDF (38%) treatment and conventional GIC restoration; group 3, CPP-ACP (3%) modified GIC; and group 4, SDF treatment and CPP-ACP modified GIC. The restored teeth were thermal-cycled before undergoing a multi-species cariogenic biofilm challenge. The restored teeth were examined by micro-computed tomography (micro-CT), scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM/EDX) and Fourier transform infrared (FTIR) spectroscopy. Data were analyzed by two-way ANOVA. Results: Micro-CT determined outer lesion depths for groups 1–4 were: 123 ± 6 μm, 87 ± 7 μm, 79 ± 3 μm and 68 ± 5 μm respectively. An interaction effect on the outer lesion depth was found between the restorative materials and SDF treatment (p < 0.001). Both SDF treatment and modification with CPP-ACP had a significant effect on outer lesion depth (p < 0.001). SEM/EDX showed an increase of calcium and phosphorus at the root dentine adjacent to the restoration in groups 3 and 4 (CPP-ACP modified GIC). FTIR revealed that SDF treatment and CPP-ACP modified GIC had a significant effect on amide I-to-hydrogen phosphate ratio on the material-root interface (p = 0.001). Conclusion: SDF treatment and incorporation of CPP-ACP into GIC restorative material can prevent secondary root caries development. Clinical significance: The results provide useful information to dentists in formulating clinical management protocols and material when treating root caries.postprin

DNA topoisomerases participate in fragility of the oncogene RET

Fragile site breakage was previously shown to result in rearrangement of the RET oncogene, resembling the rearrangements found in thyroid cancer. Common fragile sites are specific regions of the genome with a high susceptibility to DNA breakage under conditions that partially inhibit DNA replication, and often coincide with genes deleted, amplified, or rearranged in cancer. While a substantial amount of work has been performed investigating DNA repair and cell cycle checkpoint proteins vital for maintaining stability at fragile sites, little is known about the initial events leading to DNA breakage at these sites. The purpose of this study was to investigate these initial events through the detection of aphidicolin (APH)-induced DNA breakage within the RET oncogene, in which 144 APHinduced DNA breakpoints were mapped on the nucleotide level in human thyroid cells within intron 11 of RET, the breakpoint cluster region found in patients. These breakpoints were located at or near DNA topoisomerase I and/or II predicted cleavage sites, as well as at DNA secondary structural features recognized and preferentially cleaved by DNA topoisomerases I and II. Co-treatment of thyroid cells with APH and the topoisomerase catalytic inhibitors, betulinic acid and merbarone, significantly decreased APH-induced fragile site breakage within RET intron 11 and within the common fragile site FRA3B. These data demonstrate that DNA topoisomerases I and II are involved in initiating APH-induced common fragile site breakage at RET, and may engage the recognition of DNA secondary structures formed during perturbed DNA replication