Olfactory variation in mouse husbandry and its implications for refinement and standardisation: UK survey of non-animal scents

With their highly sensitive olfactory system, the behaviour and physiology of mice are not only influenced by the scents of conspecifics and other species, but also by many other chemicals in the environment. The constraints of laboratory housing limit a mouse’s capacity to avoid aversive odours that could be present in the environment. Potentially odorous items routinely used for husbandry procedures, such as sanitizing products and gloves, could be perceived by mice as aversive or attractive, and affect their behaviour, physiology and experimental results. A survey was sent to research institutions in the UK to enquire about husbandry practices that could impact on the olfactory environment of the mouse. Responses were obtained from 80 individuals working in 51 institutions. Husbandry practices varied considerably. Seventy percent of respondents reported always wearing gloves for handling mice, with nitrile being the most common glove material (94%) followed by latex (23%) and vinyl (14%). Over six different products were listed for cleaning surfaces, floors, anaesthesia and euthanasia chambers and behavioural apparatus. In all cases Trigene™ (now called Anistel™) was the most common cleaning product used (43, 41, 40 and 49%, respectively). Depending on the attribute considered, between 7 and 19% of respondents thought that cleaning products definitely, or were likely to, have strong effects on standardization, mouse health, physiology or behaviour. Understanding whether and how these odours affect mouse welfare will help to refine mouse husbandry and experimental procedures through practical recommendations, to improve the quality of life of laboratory animals and the experimental data obtained

A Radio Polarimetric Study of the Galactic Center Threads

Multi-frequency, polarimetric VLA observations of the non-thermal filaments (NTF's), G0.08+0.15, and G359.96+0.09, also known as the Northern and Southern Threads are presented at 20, 6, 3.6 and 2 cm, with high enough spatial resolution to be resolved for the first time at 6 and 3.6 cm. The 20 cm image reveals a wealth of new detail in the radio sources lying within the inner 60 pc of the Galaxy. The Southern Thread has a prominent split along its length, similar to splitting at the ends of previously studied NTF's. With resolutions as fine as 2'', the 3.6 and 6 cm images reveal a high degree of continuity and little substructure internal to the filament. The spectral index of the Northern Thread has been determined over a broad range of frequencies. Its flux density falls with frequency, alpha=-0.5 between 90 and 6 cm, and becomes much steeper (alpha=-2.0) between 6 and 2 cm. The spectral index does not vary significantly along the length of the Northern Thread, which implies either that the diffusion timescale for the emitting electrons is less than their synchrotron lifetime, or that the emitting electrons are reaccelerated continuously at multiple positions along the filament. Because of the lack of spectral index variation, we have not located the source of relativistic electrons. Polarization observations at 6 and 3.6 cm confirm the non-thermal nature of the emission from the Northern Thread. The fractional polarization in the Northern Thread reaches 70% in some regions, although the polarized emission is patchy. Large rotation measures (RM > 2000 rad/m2) have been observed with irregular variations across the filament.The intrinsic magnetic field in the Northern Thread is predominantly aligned along its long axis.Comment: 19 pages, incl. 24 figs; to appear in the Astrophysical Journa

Measurement of the Integrated Faraday Rotations of BL Lac Objects

We present the results of multi-frequency polarization VLA observations of radio sources from the complete sample of northern, radio-bright BL Lac objects compiled by H. Kuhr and G. Schmidt. These were used to determine the integrated rotation measures of 18 objects, 15 of which had never been measured previously, which hindered analysis of the intrinsic polarization properties of objects in the complete sample. These measurements make it possible to correct the observed orientations of the linear polarizations of these sources for the effect of Faraday rotation. The most probable origin for Faraday rotation in these objects is the Galactic interstellar medium. The results presented complete measurements of the integrated rotation measures for all 34 sources in the complete sample of BL Lac objects.Comment: 9 pages, 7 figure

How do I sound to me? Perceived changes in communication in Parkinson's disease

Objective: To examine self and carer perceived changes in communication associated with Parkinson's disease and relate these to speech intelligibility, gender, age and other disease measures. Design: Cross-sectional survey of a hospital- and community-based sample of 176 people with Parkinson's disease and their carers using a questionnaire based on semantic differential techniques. Participants: One hundred and four people with Parkinson's disease with no history of communication difficulties prior to onset of their Parkinson's disease and 45 primary carers who returned completed questionnaires. Main outcome measures: Differences in ratings for `before' the onset of Parkinson's disease versus present status. Results: There was a strong perception of negative impact on communication between `before' and `now', irrespective of age and gender and largely independent of disease severity and duration, intelligibility and cognitive status. Activities of daily living (assessed by Unified Parkinson's Disease Rating Scale (UPDRS) II) and depression rating scale scores had the strongest association with change (adjusted R 2 0.27). There was a significant correlation between the rank order of perceived change in features examined in people with Parkinson's disease versus their carers, though in general carers rated change as having less impact. Conclusions: Parkinson's disease exercises a strong influence on communication even before apparent alterations to intelligibility or motor status

Probing the Magnetized Interstellar Medium Surrounding the Planetary Nebula Sh 2-216

We present 1420 MHz polarization images of a 2.5 X 2.5 degree region around the planetary nebula (PN) Sh 2-216. The images are taken from the Canadian Galactic Plane Survey (CGPS). An arc of low polarized intensity appears prominently in the north-east portion of the visible disk of Sh 2-216, coincident with the optically identified interaction region between the PN and the interstellar medium (ISM). The arc contains structural variations down to the ~1 arcminute resolution limit in both polarized intensity and polarization angle. Several polarization-angle "knots" appear along the arc. By comparison of the polarization angles at the centers of the knots and the mean polarization angle outside Sh 2-216, we estimate the rotation measure (RM) through the knots to be -43 +/- 10 rad/m^2. Using this estimate for the RM and an estimate of the electron density in the shell of Sh 2-216, we derive a line-of-sight magnetic field in the interaction region of 5.0 +/- 2.0 microG. We believe it more likely the observed magnetic field is interstellar than stellar, though we cannot completely dismiss the latter possibility. We interpret our observations via a simple model which describes the ISM magnetic field around Sh 2-216, and comment on the potential use of old PNe as probes of the magnetized ISM.Comment: 25 pages, 4 figures. Accepted for publication in the Astrophysical Journa

ROSAT and ASCA observations of the Crab-Like Supernova Remnant N157B in the Large Magellanic Cloud

We report the results of ROSAT and ASCA X-ray observations of the supernova remnant N157B (or 30 Dor B, SNR 0539-69.1) in the Large Magellanic Cloud. For comparison, we also briefly describe the results on SNR 0540-69.3, the only confirmed Crab-like remnant in the Cloud. The X-ray emission from N157B can be decomposed into a bright comet-shaped feature, superimposed on a diffuse emission region of a dimension $\sim 20$ pc. The flat and nearly featureless spectrum of the remnant is distinctly different from those of young shell-like remnants, suggesting a predominantly Crab-like nature of N157B. Characterized by a power law with an energy slope $\sim 1.5$, the spectrum of N157B above $\sim 2$ keV is, however, considerably steeper than that of SNR 0540-69.3, which has a slope of $\sim 1.0$. At lower energies, the spectrum of N157B presents marginal evidence for emission lines, which if real most likely arise in hot gas of the diffuse emission region. The hot gas has a characteristic thermal temperature of 0.4-0.7 keV. No significant periodic signal is detected from N157B in the period range of $3 \times 10^{-3}-2000$ s. The pulsed fraction is $\lesssim 9$ (99% confidence) in the $2-7$ keV range. We discuss the nature of the individual X-ray components. In particular, we suggest that the synchrotron radiation of relativistic particles from a fast-moving ($\sim 10^3 km s^{-1}$) pulsar explains the size, morphology, spectrum, and energetics of the comet-shaped X-ray feature. We infer the age of the remnant as $\sim 5 \times 10^3$ yrs. The lack of radio polarization of the remnant may be due to Faraday dispersion by foreground \ion{H}{2} gas.Comment: To be published in The Astrophysical Journal, 21 pages, plus 11 images in the PS, GIF, or jpeg format. Postscript files of images are available at http://www.astro.nwu.edu/astro/wqd/paper/n157b

Self-Generated Magnetic Fields in Galactic Cooling Flows

Interstellar magnetic fields in elliptical galaxies are assumed to have their origin in stellar fields that accompany normal mass loss from an evolving population of old stars. The seed fields are amplified by interstellar turbulence driven by stellar mass loss and supernova events. These disordered fields are further amplified by time-dependent compression in the inward moving galactic cooling flow and are expected to dominate near the galactic core. Under favorable circumstances, fields similar in strength to those observed $B \sim 1-10~(r/10~kpc)^{-1.2}\mu$G can be generated solely from these natural galactic processes. In general the interstellar field throughout elliptical galaxies is determined by the outermost regions in the interstellar gas where the turbulent dynamo process can occur. Because of the long hydrodynamic flow times in galactic cooling flows, currently observed magnetic fields may result from periods of intense turbulent field amplification that occurred in the outer galaxy in the distant past. Particularly strong fields in ellipticals may result from ancient galactic mergers or shear turbulence introduced at the boundary between the interstellar gas and ambient cluster gas.Comment: 21 pages in AASTEX LaTeX with 2 figures; accepted by Astrophysical Journa

Lifetime revision risk for medial unicompartmental knee replacement is lower than expected

Purpose: Unicompartmental knee replacement (UKR) is widely considered to be a pre-total knee replacement (TKR) particularly in the young. The implication of this is that it is sensible to do a UKR, even though it will be revised at some stage, as it will delay the need for a TKR. The chance of a UKR being revised during a patient’s life time has not previously been calculated. The aim of this study was to estimate this lifetime revision risks for patients of different ages undergoing UKR. Methods: Calculations were based on data from a designer series of 1000 medial Oxford UKR with mean 10-year follow up. These UKR were implanted for the recommended indications using the recommended surgical technique. Parametric survival models were developed for patients of different ages based on observed data, and were extrapolated using a Markov model to estimate lifetime revision risk. Results: The estimated lifetime revision risk reduced with increasing age at surgery. Lifetime revision risk at age 55 was 15% (95% CI 12–19), at 65 it was 11% (8–13), at 75 it was 7% (5–9), and at 85 it was 4% (3–5). Conclusion: Provided UKR is used appropriately, the lifetime revision risk is markedly lower than expected. UKR should be considered to be a definitive knee replacement rather than a Pre-TKR even in the young. These lifetime estimates, alongside established benefits for UKR in speed of recovery, morbidity, mortality and function, can be discussed with appropriate patients when considering whether to implant a UKR or TKR. Level of Evidence:III.</p