Analytical techniques for identification and study of organic matter in returned lunar samples Semiannual report, 1 Oct. 1966 - 31 Mar. 1967

Development of computerized high resolution mass spectrometer and gas-liquid chromatographic facility for study of lunar soil sample

I WISH I WAS A COWBOY

ABSTRACT: Within my body of work, I Wish I Was A Cowboy, I consider the sociocultural norms that develop and repress sexual identities, focusing on the period of adolescence. Questionable narratives from my past and personal memories coalesce around questions of female purity, self-respect and the idea of “natural” behaviors. Video and digital image interact with sculptural structures, and together the works reference subjects of taboo, kink, and repression. Various barriers, basins, and alters reference control of bodies, the view of the public, toxic ideals of “purity” and the stigmatization that surrounds sexual power possessed by femme individuals. The title I Wish I Was a Cowboy draws attention to the devaluation that is placed on femme and femininity. Within my material choices, imagery and videos I aim to create my own femme version of being a “cowboy” situated among heavy, bleak, sterile structures. I choose to present imagery of empowerment of femme identities in sexual situations and celebration of feminine sexuality, taking visuals from images and games created by and for a masculine audience- reclaiming them in this new femme world. A considerable amount of my visual imagery encompasses a potential for choice of where the power is being held or enforced. It presents a grey area/ back and forth of disgust and desire, degradation and empowerment. Power, however, like identity, is not (or should not be) simply binary, but a fluid range. This work addresses the intricacies of sexual relationships, power relationships, and relationship with self. Using visual cues from equestrian sport, video games, and intimate acts in public spaces, I create environments that mix pleasure, frustration, vulnerability, and power

Convenient Labelling Technique for Mass Spectrometry - Acid Catalyzed Deuterium and Oxygen-18 Exchange via Gas-liquid Chromatography

Mass spectrometry labelling technique - acid catalyzed deuterium and oxygen 18 exchange by gas-liquid chromatograph

High resolution mass spectrometry in molecular structure and stereochemical studies - Effect of stereochemistry on the fragmentation of epimeric derivatives of azabicycloalkanes

High resolution mass spectrometry in studies of stereochemistry effect on fragmentation of epimeric derivatives of azabicycloalkane

Application of High Resolution Mass Spectrometry in Molecular Structure Studies

High resolution mass spectrograms of molecular structure

Impact of computer-coupled high resolution mass spectrometry on molecular structure studies

Computer-coupled high resolution mass spectrometry impact on molecular structure studie

Revealing nascent proteomics in signaling pathways and cell differentiation.

Regulation of gene expression at the level of protein synthesis is a crucial element in driving how the genetic landscape is expressed. However, we are still limited in technologies that can quantitatively capture the immediate proteomic changes that allow cells to respond to specific stimuli. Here, we present a method to capture and identify nascent proteomes in situ across different cell types without disturbing normal growth conditions, using O-propargyl-puromycin (OPP). Cell-permeable OPP rapidly labels nascent elongating polypeptides, which are subsequently conjugated to biotin-azide, using click chemistry, and captured with streptavidin beads, followed by digestion and analysis, using liquid chromatography-tandem mass spectrometry. Our technique of OPP-mediated identification (OPP-ID) allows detection of widespread proteomic changes within a short 2-hour pulse of OPP. We illustrate our technique by recapitulating alterations of proteomic networks induced by a potent mammalian target of rapamycin inhibitor, MLN128. In addition, by employing OPP-ID, we identify more than 2,100 proteins and uncover distinct protein networks underlying early erythroid progenitor and differentiation states not amenable to alternative approaches such as amino acid analog labeling. We present OPP-ID as a method to quantitatively identify nascent proteomes across an array of biological contexts while preserving the subtleties directing signaling in the native cellular environment

Liquid droplet formation by HP1α suggests a role for phase separation in heterochromatin.

Gene silencing by heterochromatin is proposed to occur in part as a result of the ability of heterochromatin protein 1 (HP1) proteins to spread across large regions of the genome, compact the underlying chromatin and recruit diverse ligands. Here we identify a new property of the human HP1α protein: the ability to form phase-separated droplets. While unmodified HP1α is soluble, either phosphorylation of its N-terminal extension or DNA binding promotes the formation of phase-separated droplets. Phosphorylation-driven phase separation can be promoted or reversed by specific HP1α ligands. Known components of heterochromatin such as nucleosomes and DNA preferentially partition into the HP1α droplets, but molecules such as the transcription factor TFIIB show no preference. Using a single-molecule DNA curtain assay, we find that both unmodified and phosphorylated HP1α induce rapid compaction of DNA strands into puncta, although with different characteristics. We show by direct protein delivery into mammalian cells that an HP1α mutant incapable of phase separation in vitro forms smaller and fewer nuclear puncta than phosphorylated HP1α. These findings suggest that heterochromatin-mediated gene silencing may occur in part through sequestration of compacted chromatin in phase-separated HP1 droplets, which are dissolved or formed by specific ligands on the basis of nuclear context