Impulse radio ultrawideband pulse shaper based on a programmable photonic chip frequency discriminator

We report and experimentally demonstrate the generation of impulse radio ultrawideband (UWB) pulses using a photonic chip frequency discriminator. The discriminator consists of three add-drop optical ring resonators (ORRs) which are fully programmable using thermo-optical tuning. This discriminator chip in combination with a phase modulator forms a temporal differentiator where phase modulation is converted to intensity modulation (PM-IM conversion). By means of tailoring the discriminator response using either the individual or the cascade of drop and through responses of the ORRs, first-order or second-order temporal differentiations are obtained. Using this principle, the generation of UWB monocycle, doublet and modified doublet pulses are demonstrated. The use of this CMOS-compatible discriminator is promising for the realization of a compact and low cost UWB transmitter

The analysis of pendolino (peo) mutants reveals differences in the fusigenic potential among Drosophila telomeres

Drosophila telomeres are sequence-independent structures that are maintained by transposition to chromosome ends of three specialized retroelements (HeT-A, TART and TAHRE; collectively designated as HTT) rather than telomerase activity. Fly telomeres are protected by the terminin complex (HOAP-HipHop-Moi-Ver) that localizes and functions exclusively at telomeres and by non-terminin proteins that do not serve telomere-specific functions. Although all Drosophila telomeres terminate with HTT arrays and are capped by terminin, they differ in the type of subtelomeric chromatin; the Y, XR, and 4L HTT are juxtaposed to constitutive heterochromatin, while the XL, 2L, 2R, 3L and 3R HTT are linked to the TAS repetitive sequences; the 4R HTT is associated with a chromatin that has features common to both euchromatin and heterochromatin. Here we show that mutations in pendolino (peo) cause telomeric fusions (TFs). The analysis of several peo mutant combinations showed that these TFs preferentially involve the Y, XR and 4th chromosome telomeres, a TF pattern never observed in the other 10 telomere-capping mutants so far characterized. peo encodes a non-terminin protein homologous to the E2 variant ubiquitin-conjugating enzymes. The Peo protein directly interacts with the terminin components, but peo mutations do not affect telomeric localization of HOAP, Moi, Ver and HP1a, suggesting that the peodependent telomere fusion phenotype is not due to loss of terminin from chromosome ends. peo mutants are also defective in DNA replication and PCNA recruitment. However, our results suggest that general defects in DNA replication are unable to induce TFs in Drosophila cells. We thus hypothesize that DNA replication in Peodepleted cells results in specific fusigenic lesions concentrated in heterochromatinassociated telomeres. Alternatively it is possible that Peo plays a dual function being independently required for DNA replication and telomere capping

Integrated photonic K_u-band beamformer chip with continuous amplitude and delay control

We present the first demonstration of a broadband and continuously tunable integrated optical beamforming network (IOBFN) capable of providing continuously tunable true-time-delay up to 236 ps over the entire DVB-S band (10.7–12.75 GHz), realized with a CMOS compatible process. The tunable delays are based on reconfigurable optical ring resonators in conjunction with a single optical sideband filter integrated on the same optical chip. The delays and filter responses are software programmable. Four tunable delay lines are integrated on a single chip and configured to feed a 16-element linear antenna array. The broadband beam steering capability of the proposed IOBFN is demonstrated by the squint-free antenna pattern generated from the measured RF amplitude and phase responses of the optical delay line

Novel ring resonator-based integrated photonic beamformer for broadband phased array receive antennas - part I: design and performance analysis

A novel optical beamformer concept is introduced that can be used for seamless control of the reception angle in broadband wireless receivers employing a large phased array antenna (PAA). The core of this beamformer is an optical beamforming network (OBFN), using ring resonator-based broadband delays, and coherent optical combining. The electro-optical conversion is performed by means of single-sideband suppressed carrier modulation, employing a common laser, Mach-Zehnder modulators, and a common optical sideband filter after the OBFN. The unmodulated laser signal is then re-injected in order to perform balanced coherent optical detection, for the opto-electrical conversion. This scheme minimizes the requirements on the complexity of the OBFN, and has potential for compact realization by means of full integration on chip. The impact of the optical beamformer concept on the performance of the full receiver system is analyzed, by modeling the combination of the PAA and the beamformer as an equivalent two-port RF system. The results are illustrated by a numerical example of a PAA receiver for satellite TV reception, showing that—when properly designed—the beamformer hardly affects the sensitivity of the receiver

The telomeric protein AKTIP interacts with A- and B-type lamins and is involved in regulation of cellular senescence

AKTIP is a shelterin-interacting protein required for replication of telomeric DNA. Here, we show that AKTIP biochemically interacts with A- and B-type lamins and affects lamin A, but not lamin C or B, expression. In interphase cells, AKTIP localizes at the nuclear rim and in discrete regions of the nucleoplasm just like lamins. Double immunostaining revealed that AKTIP partially co-localizes with lamin B1 and lamin A/C in interphase cells, and that proper AKTIP localization requires functional lamin A. In mitotic cells, AKTIP is enriched at the spindle poles and at the midbody of late telophase cells similar to lamin B1. AKTIP-depleted cells show senescence-associated markers and recapitulate several aspects of the progeroid phenotype. Collectively, our results indicate that AKTIP is a new player in lamin-related processes, including those that govern nuclear architecture, telomere homeostasis and cellular senescence

AKTIP/Ft1, a new shelterin-interacting factor required for telomere maintenance

Telomeres are nucleoprotein complexes that protect the ends of linear chromosomes from incomplete replication, degradation and detection as DNA breaks. Mammalian telomeres are protected by shelterin, a multiprotein complex that binds the TTAGGG telomeric repeats and recruits a series of additional factors that are essential for telomere function. Although many shelterin-associated proteins have been so far identified, the inventory of shelterin-interacting factors required for telomere maintenance is still largely incomplete. Here, we characterize AKTIP/Ft1 (humanAKTIP and mouse Ft1 are orthologous), a novel mammalian shelterin-bound factoridentified on the basis of its homology with the Drosophila telomere protein Pendolino. AKTIP/Ft1 shares homology with the E2 variant ubiquitin-conjugating (UEV) enzymes and has been previously implicated in the control of apoptosis and in vesicle trafficking. RNAi-mediated depletion of AKTIP results in formation of telomere disfunction foci (TIFs). Consistent with these results, AKTIP interacts with telomeric DNA and binds the shelterin components TRF1 and TRF2 both in vivo and in vitro. Analysis of AKTIP- depleted human primary fibroblasts showed that they are defective in PCNA recruiting and arrest in the S phase due to the activation of the intra S checkpoint. Accordingly, AKTIP physically interacts with PCNA and the RPA70 DNA replication factor. Ft1-depleted p53-/- MEFs did not arrest in the S phase but displayed significant increases in multiple telomeric signals (MTS) and sister telomere associations (STAs), two hallmarks of defective telomere replication. In addition, we found an epistatic relation for MST formation between Ft1 and TRF1, which has been previously shown to be required for replication fork progression through telomeric DNA. Ch-IP experiments further suggested that in AKTIP-depleted cells undergoing the S phase, TRF1 is less tightly bound to telomeric DNA than in controls. Thus, our results collectively suggest that AKTIP/Ft1 works in concert with TRF1 to facilitate telomeric DNA replication

Mice with reduced expression of the telomere-associated protein Ft1 develop p53-sensitive progeroid traits

Human AKTIP and mouse Ft1 are orthologous ubiquitin E2 variant proteins involved in telomere maintenance and DNA replication. AKTIP also interacts with A- and B-type lamins. These features suggest that Ft1 may be implicated in aging regulatory pathways. Here, we show that cells derived from hypomorph Ft1 mutant (Ft1kof/kof ) mice exhibit telomeric defects and that Ft1kof/kof animals develop progeroid traits, including impaired growth, skeletal and skin defects, abnormal heart tissue, and sterility. We also demonstrate a genetic interaction between Ft1 and p53. The analysis of mice carrying mutations in both Ft1 and p53 (Ft1kof/kof ; p53ko/ko and Ft1kof/kof ; p53+/ko ) showed that reduction in p53 rescues the progeroid traits of Ft1 mutants, suggesting that they are at least in part caused by a p53-dependent DNA damage response. Conversely, Ft1 reduction alters lymphomagenesis in p53 mutant mice. These results identify Ft1 as a new player in the aging process and open the way to the analysis of its interactions with other progeria genes using the mouse model

Advanced integrated optical beam forming networks for broadband phased array antenna systems

Since the first half of the twentieth century, a large interest has been addressed by the scientific and engineering community to the world of phased arrays antennas. Their technology started to be developed during the Second World War for early warning radar techniques to identify threats from the skies. Those capable antennas have been deployed over the years in a number of diversified fields to address different applications needs. First employed almost exclusively in the defense and space domains, nowadays the increased need for broadband connectivity in personal communications is leveraging their diffusion to the field of smart antennas for modern wireless communications.\ud This thesis deals with a special way to fully exploit and further enhance the high flexibility of those complex yet very powerful type of antennas, mainly in terms of speed, compactness and cost. A number of fields have benefited the extremely low loss and broad bandwidth of optical communication technology. Especially interesting to us are the capabilities and extended possibilities brought to the world of microwave and antenna engineering by the use of optics and, specifically, of integrated photonics. This field is known as integrated microwave photonics. The huge bandwidth and extreme low loss of optical fibers can be harnessed to implement very capable signal processing circuits for high-frequency electrical signals. In particular, very broadband and continuosly tunable time delay units can be realized optically; those are of fundamental interest in a number of signal processing functionalities. Specifically, our interest in this work has been the design, realization, testing and integration in complex systems of novel beamformers that employ those delay lines, in conjunction with other components, to control the direction of radiation of a high-performance antenna array. When realized with integrated optical technology, we talk about integrated optical beamformers

Development, test and flight results of the rf systems for the yes2 tether experiment

This paper highlights design, realization, testing and flight results of the Radio Frequency developments (RF) for ESA's second Young Engineers' Satellite (YES2), that included GPS systems, an intersatellite UHF link and a re-entry capsule telemetry and recovery system. The YES2 piggybacked on the Russian-built Foton microgravity platform in September 2007 with as objective the controlled deployment of a 32 km tether, in order to release a small re-entry capsule. That mission was successfully performed, although the capsule could not yet be retrieved. This complex project was performed by hundreds of students from all over Europe. One of the student teams was concerned with the development of RF systems. Most significant is the inter-satellite link between the tethered sub satellite and the tether deployed, through which tether science data from the sub satellite (e.g. tensiometer and dynamic sensors) can be recovered. Furthermore, a GPS/GLONASS receiver was placed on both ends of the tether to monitor the tether deployment. Next, the re-entry capsule has its own transmitter which sends data to an especially developed mobile ground station. Finally, recovery of the landed capsule was planned using an ARGOS beacon, for which two DDRR (loop antennas) were designed that transmit the beacon signal to the ARGOS constellation for positioning. This paper provides a brief overview of these systems and tests performed, and concludes with a discussion of flight results and applicability of the systems to other low-cost satellites, balloon experiments or sounding rockets.\u