DETECTION OF ANTIBODIES AGAINST PARASCARIS EQUORUM EXCRETORY-SECRETORY ANTIGENS

Parascaris equorum is a nematode parasite that infects young horses, sometimes causing unthriftiness, respiratory signs, or intestinal impaction in severe cases. Infection can be diagnosed by detection of eggs in feces, but this is only possible after the worms are fully mature. The goal of this study was to develop an antibody-based test for prepatent diagnosis of P. equorum infection. To produce western blot (WB) antigen, P. equorum larvae were cultured for collection of excretory-secretory antigens (ESA). Sera from 18 pregnant broodmares, their subsequent foals, and a group of 12 older mares and geldings were analyzed. In order to check for cross-reactivity between P. equorum and other ascarid species and equine parasites, additional sera were analyzed. Sera from a horse with monospecific P. equorum infection was compared to horses with monospecific Strongyloides westeri or cyathostome infections, rabbits inoculated with Baylisascaris procyonis or Toxocara canis eggs, dogs naturally infected with T. canis, and rabbits immunized with B. procyonis or P. equorum ESA. Molecular weights of silver-stained P. equorum larval ESA ranged between 12 to 94 kDa. In WB analysis, sera from 94% of broodmares contained IgG(T) antibody that recognized multiple P. equorum larval ESA. Foals showed no IgG(T) antibodies pre-suckle, but antibodies similar to their dams were observed post-suckle and thereafter. Of the older mares and geldings, 58% had IgG(T) antibodies recognizing larval ESA. Serum IgG(T) antibodies against P. equorum larval ESA were also found in parasite-free and monospecific infection equine sera. Ascarid positive foals did not produce detectable amounts of IgE or IgM antibodies against larval ESA. When P. equorum, T. canis, and B. procyonis antibody reactivity was compared, antigens at 19 kDa and 34 kDa had the highest potential for identification of larval P. equorum infections. When immature adult P. equorum ESA was examined, IgG(T) antibody recognition was demonstrated in 50% of broodmares and 17% of the older horses, and appeared several weeks prior to patency in foal serum. Results indicate that IgG(T) antibodies against P. equorum ESA are common in mature horses, and are transferred from mare to foal, limiting the diagnostic potential of an antibody-based test

Controlling external pests of livestock

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4-H for safety

September, 1947."...Much of the material has been provided through the courtesy of the Farm Section of the National Safety Council."--Page 3."University of Missouri College of Agriculture and the United States Department of Agriculture Cooperating"--Page 20.Title from cover

The sow and litter : 4-H project

At head of title: University of Missouri College of Agriculture, Agricultural Extension Service."January, 1947.""University of Missouri College of Agriculture and the United States Department of Agriculture Cooperating"--Page 24.Title from cover

Visual, Optical and Replica Inspections: Surface Preparation of 650 MHz NB Cavity for PIP-II Linac

Surface preparation of niobium superconducting RF cavities is a critical step for achieving good RF performance under the superconducting state. Surface defect, roughness, and contamination affect the accelerating gradient and quality factor of the cavities. We report surface inspection methods used to control the surface processing of 650 MHz cavities designated for the pre-production and prototype cryomodules for PIP-II linac. The cavity surface was routinely inspected visually, with an optical camera, and by microscopic scanning of surface replicas. This article covers details on the surface inspection methods and surface polishing process used to repair the surface

Trends and determinants of postabortion contraception use in a Canadian retrospective cohort.

OBJECTIVES: We aim to describe demographic trends associated with postabortion contraceptive choice, characteristics of intrauterine device (IUD) users and relation to subsequent abortion. STUDY DESIGN: Our retrospective chart review study included all patients obtaining an abortion from 2003 to 2010 at the primary service provider in the Interior Health Region of British Columbia, tracking each patient for 5 years to detect subsequent abortion. We used descriptive statistics to analyze demographic trends and logistic regression to examine determinants of choosing an IUD and likelihood of subsequent abortion per contraceptive method. RESULTS: Our study cohort included 5206 patients, 1247 (24.0%) of whom chose an IUD. Patients increased IUD use from 10.14% to 45.74% of the cohort over the study period. Mean age of those choosing an IUD significantly decreased over the study period [30.9±7.3 years in 2003 to 26.2±6.5 years in 2010 (p<.001)]. In multivariable analysis, factors associated with choosing an IUD postabortion were prior delivery [aOR=2.77 (95% CI 2.40-3.20)] and being older than 20 years [20-29 years: AOR=1.87 (1.51-2.32); or 30+ years: AOR=1.96 (1.54-2.50)]. Patients choosing an IUD were less likely to have a subsequent abortion compared to those selecting oral contraceptives [aOR=1.96 (95% CI 1.54-2.52)] or depomedroxyprogesterone acetate [aOR=1.84 (95% CI 1.36-2.49)]. CONCLUSIONS: We found an increasing trend of choosing an IUD after an abortion in our population, especially among youth. Patients who chose an IUD postabortion were less likely to have a subsequent abortion over the next 5 years. IMPLICATIONS: An important strategy for reducing subsequent abortion is to ensure that those seeking abortion have accurate information on the comparative effectiveness of postabortion contraception methods. Educational efforts, alongside removal of cost and other barriers, will contribute to the prevention of subsequent abortion and improve equitable access to IUDs among the population

Scalable Production of Equine Platelet Lysate for Multipotent Mesenchymal Stromal Cell Culture

Translation of multipotent mesenchymal stromal cell (MSC)-based therapies is advancing in human and veterinary medicine. One critical issue is the in vitro culture of MSC before clinical use. Using fetal bovine serum (FBS) as supplement to the basal medium is still the gold standard for cultivation of many cell types including equine MSC. Alternatives are being explored, with substantial success using platelet lysate-supplemented media for human MSC. However, progress lags behind in the veterinary field. The aim of this study was to establish a scalable protocol for equine platelet lysate (ePL) production and to test the ePL in equine MSC culture. Whole blood was harvested into blood collection bags from 20 healthy horses. After checking sample materials for pathogen contamination, samples from 19 animals were included. Platelet concentrates were prepared using a buffy coat method. Platelets, platelet-derived growth factor BB, and transforming growth factor b1 concentrations were increased in the concentrates compared with whole blood or serum (p < 0.05), while white blood cells were reduced (p < 0.05). The concentrates were lysed using freeze/thaw cycles, which eliminated the cells while growth factor concentrations were maintained. Donor age negatively correlated with platelet and growth factor concentrations after processing (p < 0.05). Finally, all lysates were pooled and the ePL was evaluated as culture medium supplement in comparison with FBS, using adipose-derived MSC from four unrelated donor horses. MSC proliferated well in 10% FBS as well as in 10% ePL. However, using 5 or 2.5% ePL entailed highly inconsistent proliferation or loss of proliferation, with significant differences in generation times and confluencies (p < 0.05). MSC expressed the surface antigens CD90, CD44, and CD29, but CD73 and CD105 detection was low in all culture media. Adipogenic and osteogenic differentiation led to similar results in MSC from different culture media. The buffy coat method is useful to produce equine platelet concentrate with increased platelet and reduced white blood cell content in large scales. The ePL obtained supports MSC expansion similar as FBS when used at the same concentration (10%). Further investigations into equine MSC functionality in culture with ePL should follow

Hanford Site Climatological Data Summary 2000 with Historical Data

This document presents the climatological data measured at the U.S. Department of Energy's Hanford Site for calendar year 2000