A community view of smoking cessation counseling in the practices of physicians and dentists.

The practice norms of community physicians and dentists in the Lehigh Valley of Pennsylvania for counseling about smoking cessation were surveyed. In addition, 1,373 residents in the valley were interviewed by telephone about the smoking counseling behaviors of their dentists and physicians. These activities were conducted as part of the planning for an intervention by the Coalition for a Smoke-Free Valley, a coalition of 100 persons and organizations in the area. The survey response rate for 172 physicians was 77 percent, and for 103 dentists, it was 76 percent. More physicians than dentists advised patients to quit, counseled patients, provided materials, and helped the patient to set a quit date. However, there was a clear discrepancy between what physicians say they do and what smokers say they hear

Yearling Beef Cattle Grazing Diverse Summer Annual Swards

Utilizing summer annuals is often regarded as “a breakeven proposition at best” due to high establishment costs. This study investigated using botanical diversity to increase forage yield or animal performance to improve the economic feasibility of grazing summer annual forages in western Kentucky, USA. Sorghum-sudangrass (Sorghum bicolor x S. bicolor spp. drummondii)-based treatments included a monoculture, a three-species mixture (two grasses, one legume), and a 12-species mixture (five grasses, four legumes, two brassicas, and one forb). Angus-cross yearling beef calves (329, 366, and 297 kg in 2017, 2018, and 2019, respectively) grazed summer annuals in 2017-2019 for an average of 40 days each year without supplementation. Pastures were stocked when forage was approximately 1 m tall and calves were removed when forage was 2-2.5 m tall (seedheads present). In all years, forage dry matter yield was not different between treatments (p \u3e 0.85) and both mixtures were dominated by sorghum-sudangrass. In 2017 (p \u3c 0.03) and 2019 (p \u3c 0.03), calves grazing the 12-species mixture had lower average daily gains (ADG) than the monoculture and 3-species mixture, while there was no difference in 2018 (p \u3e 0.3). Average daily gains were suboptimal for stocker calves in all years (0.75, 0.01, 0.54 kg day-1 in 2017, 2018, and 2019). The extremely low ADG in 2018 was likely a result of stocking pastures late in the season when grasses were at physiological maturity. Additional species increased seed cost but did not contribute significantly to forage production and did not result in increased animal production. Unless greater forage yield or livestock gains are attained, planting mixtures may not provide any economic benefit. However, adjusting seeding rates to favor less dominant species may provide a more accurate representation of species diversity manipulation effects on forage and livestock production

Integration of Nodal and BMP Signals in the Heart Requires FoxH1 to Create Left–Right Differences in Cell Migration Rates That Direct Cardiac Asymmetry

Failure to properly establish the left–right (L/R) axis is a major cause of congenital heart defects in humans, but how L/R patterning of the embryo leads to asymmetric cardiac morphogenesis is still unclear. We find that asymmetric Nodal signaling on the left and Bmp signaling act in parallel to establish zebrafish cardiac laterality by modulating cell migration velocities across the L/R axis. Moreover, we demonstrate that Nodal plays the crucial role in generating asymmetry in the heart and that Bmp signaling via Bmp4 is dispensable in the presence of asymmetric Nodal signaling. In addition, we identify a previously unappreciated role for the Nodal-transcription factor FoxH1 in mediating cell responsiveness to Bmp, further linking the control of these two pathways in the heart. The interplay between these TGFβ pathways is complex, with Nodal signaling potentially acting to limit the response to Bmp pathway activation and the dosage of Bmp signals being critical to limit migration rates. These findings have implications for understanding the complex genetic interactions that lead to congenital heart disease in humans

DYX1C1 is required for axonemal dynein assembly and ciliary motility

Dyx1c1 has been associated with dyslexia and neuronal migration in the developing neocortex. Unexpectedly, we found that deletion of Dyx1c1 exons 2–4 in mice caused a phenotype resembling primary ciliary dyskinesia (PCD), a genetically heterogeneous disorder characterized by chronic airway disease, laterality defects, and male infertility. This phenotype was confirmed independently in mice with a Dyx1c1c.T2A start codon mutation recovered from an ENU mutagenesis screen. Morpholinos targeting dyx1c1 in zebrafish also created laterality and ciliary motility defects. In humans, recessive loss-of-function DYX1C1 mutations were identified in twelve PCD individuals. Ultrastructural and immunofluorescence analyses of DYX1C1-mutant motile cilia in mice and humans revealed disruptions of outer and inner dynein arms (ODA/IDA). DYX1C1 localizes to the cytoplasm of respiratory epithelial cells, its interactome is enriched for molecular chaperones, and it interacts with the cytoplasmic ODA/IDA assembly factor DNAAF2/KTU. Thus, we propose that DYX1C1 is a newly identified dynein axonemal assembly factor (DNAAF4)

Influence of hydraulic property correlation on predicted dense nonaqueous phase liquid source zone architecture, mass recovery and contaminant flux

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/94685/1/wrcr9972.pd

Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation

Collagen is a major component of the extracellular matrix and its integrity is essential for connective tissue and organ function. The importance of proteins involved in intracellular collagen post-translational modification, folding and transport was recently highlighted from studies on recessive forms of osteogenesis imperfecta (OI). Here we describe the critical role of SC65 (Synaptonemal Complex 65, P3H4), a leprecan-family member, as part of an endoplasmic reticulum (ER) complex with prolyl 3-hydroxylase 3. This complex affects the activity of lysyl-hydroxylase 1 potentially through interactions with the enzyme and/or cyclophilin B. Loss of Sc65 in the mouse results in instability of this complex, altered collagen lysine hydroxylation and cross-linking leading to connective tissue defects that include low bone mass and skin fragility. This is the first indication of a prolyl-hydroxylase complex in the ER controlling lysyl-hydroxylase activity during collagen synthesis