Bilateral ovarian ischemia/reperfusion injury and treatment options in rats with an induced model of diabetes

WOS: 000345352100010PubMed: 24904723Objective(s): This study investigated the effects of melatonin, famotidine, mirtazapine, and thiamine pyrophosphate on ischemia/ reperfusion (I/ R) injury in diabetic rats and evaluated oxidant and antioxidant marker measurement results. It also examined the effects of the drugs aimed at preventing infertility that may result from I/ R injury. Materials and Methods: Diabetic rats were divided into a control group (IRC) to be exposed to I/ R, an ovarian I/ R + 2.2 mg/ kg melatonin (IRML) group, an ovarian I/ R + famotidine (IRFA) group, an ovarian I/ R + 20 mg/ kg mirtazapine (IRMR) group, an ovarian I/ R + 20 mg/ kg thiamine pyrophosphate (IRTP) group, and a sham operation (SO) group. Results: in the control group exposed to I/ R, the levels of the oxidant parameters Malondialdehyde (MDA) and Myeloperoxidase (MPO) were significantly higher compared with the SO group, while the levels of the antioxidant parameters glutathione (GSH), Glutathione peroxidase (GPO), Glutathione reductase (GSHRd), Glutathione S - transferase (GST), and Superoxide dismutase (SOD) were significantly lower. Melatonin, famotidine, mirtazapine, and thiamin pyrophosphate prevented a rise in oxidant parameters and a decrease in antioxidants in ovarian tissue exposed to I/R. However, apart from thiamin pyrophosphate, none of the drugs were able to prevent infertility caused by I/R injury. Conclusion: Prevention of ovarian I/R injury-related infertility in rats with induced diabetes is not through antioxidant activity. Thiamine pyrophosphate prevents infertility through an as yet unknown mechanism. This study suggests that thiamine pyrophosphate may be useful in the prevention of I/R-related infertility in diabetics

The effect of Lacidipine on Ischemia-Reperfusion Induced oxidative damage in ovaries of female rats.

WOS: 000318217500006In this study, we investigated the effects of lacidipine on XO, MDA, MPO, and GSH levels in ovarian tissues of rats, which are subjected to ischemia and post-ischemic reperfusion. In ovarian ischemia (OI), ovarian ischemia-reperfusion (OIR), 2 mg/kg lacidipine + ovarian ischemia (LOI-2), 4 mg/kg lacidipine + ovarian ischemia (LOI-4), 2 mg/kg lacidipine + ovarian ischemia-reperfusion (LOIR-2), 4 mg/kg lacidipine + ovarian ischemia-reperfusion (LOIR-4) and sham-operated healthy control group (HCG); XO 23.8 +/- 0.94, 13.6 +/- 0.76, 8 +/- 0.57, 5.8 +/- 0.60, 4.5 +/- 0.42, 3.6 +/- 0.55, 2.8 +/- 0.30 u/g, respectively, whereas MDA, 4.8 +/- 0.47, 13.33 +/- 0.66, 3.5 +/- 0.35, 3.4 +/- 0.42, 6.0 +/- 0.57, 5.3 +/- 0.61,2.8 +/- 0.22 mu mol/g protein, respectively. MPO, 6.1 +/- 0.60, 14.1 +/- 0.94, 6.3 +/- 0.66, 6.0 +/- 0.73, 8.8 +/- 0.60, 7.5 +/- 0.76, 6 +/- 1.06 u/g protein, respectively, while GSH 8.0 +/- 0.73, 3.1 +/- 0.47, 8.6 +/- 0.95, 8.9 +/- 0.91, 7.1 +/- 0.60, 7.5 +/- 0.76 ,9.1 +/- 0.47 nmol/g protein, respectively. The present study, conducted on the basis of the mechanism of formation of IR injury, demonstrated that lacidipine prevents ischemia-reperfusion injury in the ovarian tissues