AMTRAN development program Interim report

AMTRAN software system developed for IBM 1130 compute

EVALUATION OF THE METABOLIC RESPONSE, BLOOD CHEMISTRY PROFILE, AND CARDIAC MORPHOLOGY OF BEEF HEIFERS SUPPLEMENTED WITH ZILPATEROL HYDROCHLORIDE FOLLOWED BY AN ENDOCRINE INDUCED STRESS CHALLENGE

The objective of this study was to determine the metabolic, stress, hematology, organ weights, histology and cardiac anatomical features of beef heifers supplemented with zilpaterol hydrochloride (ZH) when exposed to a corticotropin-releasing hormone (CRH) and arginine vasopressin (VP) challenge. Crossbred heifers (n = 20; 556 ± 7 kg BW) were randomized into two treatment groups: 1) Control (CON): no ZH, and 2) Zilpaterol (ZIL): supplemented with ZH at 8.33 mg / kg (DM basis). The ZIL group was supplemented ZH for 20 d, with a 3-d withdrawal period. On d 24, heifers received an intravenous bolus of CRH (0.3 µg / kg BW) and VP (1.0 µg / kg BW). Blood samples were collected at 30- and 60-min intervals for serum, and 60-min intervals for plasma and whole blood, from -2 to 8 h relative to the challenge. Heifers were harvested to collect organ weights and histology samples on d 25, 26, and 27. There was a treatment (P \u3c 0.001) effect for vaginal temperature (VT), with ZIL having a 0.1oC decrease in VT. A treatment x time effect (P = 0.002) was observed for non-esterified fatty acid such that concentrations were increased in CON heifers at 4, 6, 7, 7.5, and 8 hrs. There was a treatment effect for cortisol (P \u3c 0.01) and epinephrine (P = 0.003) with ZIL having decreased concentrations. Glucose concentrations within the longissimus muscle were greater (P = 0.03) in CON heifers. Lactate concentrations and glycolytic potential within the biceps femoris were greater in CON heifers (P = 0.05). A treatment effect (P ≤ 0.02) was observed for calcium and potassium, with ZIL heifers having decreased concentrations. Zilpaterol heifers had increased concentrations of creatinine (P = 0.02). A treatment x time interaction (P = 0.02) was observed for phosphorus. Raw liver weights tended (P = 0.08) to be less in ZIL heifers. These data suggest some variations between treatments in terms of response to ZH supplementation and challenge; however, in the environmental conditions of this study, the data suggests that the supplementation of ZH does not negatively alter the status of homeostasis in cattle. Advisor: Ty B. Schmid

EVALUATION OF THE METABOLIC RESPONSE, BLOOD CHEMISTRY PROFILE, AND CARDIAC MORPHOLOGY OF BEEF HEIFERS SUPPLEMENTED WITH ZILPATEROL HYDROCHLORIDE FOLLOWED BY AN ENDOCRINE INDUCED STRESS CHALLENGE

The objective of this study was to determine the metabolic, stress, hematology, organ weights, histology and cardiac anatomical features of beef heifers supplemented with zilpaterol hydrochloride (ZH) when exposed to a corticotropin-releasing hormone (CRH) and arginine vasopressin (VP) challenge. Crossbred heifers (n = 20; 556 ± 7 kg BW) were randomized into two treatment groups: 1) Control (CON): no ZH, and 2) Zilpaterol (ZIL): supplemented with ZH at 8.33 mg / kg (DM basis). The ZIL group was supplemented ZH for 20 d, with a 3-d withdrawal period. On d 24, heifers received an intravenous bolus of CRH (0.3 µg / kg BW) and VP (1.0 µg / kg BW). Blood samples were collected at 30- and 60-min intervals for serum, and 60-min intervals for plasma and whole blood, from -2 to 8 h relative to the challenge. Heifers were harvested to collect organ weights and histology samples on d 25, 26, and 27. There was a treatment (P \u3c 0.001) effect for vaginal temperature (VT), with ZIL having a 0.1oC decrease in VT. A treatment x time effect (P = 0.002) was observed for non-esterified fatty acid such that concentrations were increased in CON heifers at 4, 6, 7, 7.5, and 8 hrs. There was a treatment effect for cortisol (P \u3c 0.01) and epinephrine (P = 0.003) with ZIL having decreased concentrations. Glucose concentrations within the longissimus muscle were greater (P = 0.03) in CON heifers. Lactate concentrations and glycolytic potential within the biceps femoris were greater in CON heifers (P = 0.05). A treatment effect (P ≤ 0.02) was observed for calcium and potassium, with ZIL heifers having decreased concentrations. Zilpaterol heifers had increased concentrations of creatinine (P = 0.02). A treatment x time interaction (P = 0.02) was observed for phosphorus. Raw liver weights tended (P = 0.08) to be less in ZIL heifers. These data suggest some variations between treatments in terms of response to ZH supplementation and challenge; however, in the environmental conditions of this study, the data suggests that the supplementation of ZH does not negatively alter the status of homeostasis in cattle. Advisor: Ty B. Schmid

Fatty Acid Composition of Beef Fed OmniGen- AF at Receiving or Finishing

Beef fatty acid profiles and superoxide dismutase activity were determined for cattle receiving OmniGen- AF supplementation (a patented nutritional supplement) at receiving (first 28 d at the feedlot) or throughout finishing (all 210 d of finishing) vs. a control group (non- supplemented). Th e most meaningful change in fatty acid composition from inclusion of OmniGen- AF was total poly- unsaturated fatty acid (PUFA) content where beef from the finishing group had more PUFA content in relation to the receiving group and was not different from the control group. Despite this increase in PUFA, cattle supplemented through finishing tended to have less lipid oxidation than the other two treatments yet this difference could not be explained by the superoxide dismutase activity

Scaled Quail Reproduction in the Trans-Pecos Region of Texas

Scaled quail (Callipepla squamata) populations have declined markedly throughout their range. We monitored hatch rates and nest placement of radio-marked female scaled quail (n 1⁄4 210) in Pecos County, Texas relative to the availability and location of ‘spreader dams’ (i.e., shallow water catchments) through the nesting seasons of 1999 and 2000. Hatch rates were high both years (i.e., 67 and 84% for 1999 and 2000, respectively). The predominant nesting microhabitat was tobosa (Pleuraphis mutica), which accounted for 85% of the nests located. We failed to document any direct impacts of spreader dams on nesting ecology of scaled quail

Effect of feeding distillers grains during different phases of production and addition of postmortem antioxidants on shelf life of ground beef1

Feeding distillers grains (DGS) to cattle can increase PUFA concentration, increase lipid oxidation, and de- crease color stability of beef. The objective of this study was to evaluate the effects of feeding DGS and the post- mortem addition of antioxidants on the shelf life of ground beef products. Crossbred heifers (n = 64; initial BW = 225 kg) were supplemented with different amounts of modi- fied DGS (MDGS; 0.91 or 2.27 kg daily, DM basis) dur- ing backgrounding and finished on diets containing corn gluten feed or MDGS. Four beef shoulder clods from each dietary group were ground independently. Fatty acid com- position was analyzed in lean tissue, s.c. fat, and compos- ite samples. Raw patties in retail display were analyzed for lipid oxidation, percent discoloration, and objective color. Cooked beef links were manufactured with salt, phosphate, and varying quantities of an antioxidant (rose- mary and green tea extract), and lipid oxidation was mea- sured throughout storage. Finishing cattle fed MDGS had greater C18:2 and PUFA (P ≤ 0.028) content in all loca- tions, whereas cattle supplemented with greater amounts of MDGS during backgrounding had more C18:0 (P = 0.005) and less C16:1 (P = 0.020) in s.c. fat. Raw ground beef from heifers finished with MDGS discolored at a greater rate (P \u3c 0.001), but lipid oxidation was not dif- ferent (P = 0.47). Greater lipid oxidation in cooked beef links occurred when cattle were fed greater amounts of MDGS during backgrounding or MDGS during finishing, but adding the rosemary and green tea extract decreased lipid oxidation regardless of dietary treatment

Effect of Feeding Different Types of Byproducts and Concentrations Throughout a Beef Growing System on Ground Beef Color and Lipid Oxidation

The objective of this trial was to evaluate the effect of feeding different concentrations of wet distillers grains during winter backgrounding and either modified wet distillers grains or Sweet Bran® during the finishing phase on ground beef color and lipid oxidation. After a 14 day aging period, ground beef patties were made and placed in a simulated retail display for seven days. There were no overall differences in lipid oxidation between treatments but was a treatment by day interaction for discoloration. Ground beef from heifers finished with modified wet distillers grains discolored at a greater extent when compared to ground beef from heifers finished with Sweet Bran

Impact of Supplementing Cattle with OmniGen- AF at the Receiving or Finishing Phase on Beef Shelf- Life

A patented proprietary product, OmniGen- AF, was evaluated to extend beef steak shelf- life at 8, 22 and 29 days of aging. Th e three treatment groups included a control group with no supplementation, OmniGen- AF supplementation for28 d aft er receiving, and supplementation for 210 d during finishing. Th e inclusion of OmniGen- AF had no effect on tenderness or visual discoloration under retail display conditions and minimal effects on fatty acid composition. Although color stability seemed to be unaffected by the supplementation, feeding OmniGen- AF throughout the entire feeding period tended to decrease oxidation. Supplementing cattle with a greater concentration of OmniGen- AF or increasing the antioxidant components in the feed supplement could be explored to further maximize beef shelf- life following long periods of aging

Fatty Acid Composition of Beef Fed OmniGen- AF at Receiving or Finishing

Beef fatty acid profiles and superoxide dismutase activity were determined for cattle receiving OmniGen- AF supplementation (a patented nutritional supplement) at receiving (first 28 d at the feedlot) or throughout finishing (all 210 d of finishing) vs. a control group (non- supplemented). Th e most meaningful change in fatty acid composition from inclusion of OmniGen- AF was total poly- unsaturated fatty acid (PUFA) content where beef from the finishing group had more PUFA content in relation to the receiving group and was not different from the control group. Despite this increase in PUFA, cattle supplemented through finishing tended to have less lipid oxidation than the other two treatments yet this difference could not be explained by the superoxide dismutase activity

Evaluation of the Immune Response of Angus Heifers with Different Genetic Markers for Marbling when Challenged with Lipopolysaccharide

Nineteen heifers (274 ± 24 kg) were blocked into two treatment groups based upon DNAm; heifers with no DNAm (noQG), and heifers with one or more DNAm (1+QG). Prior to challenge (24 h), heifers were fitted with indwelling jugular catheters and indwelling vaginal temperature (VT) monitoring devices. Blood samples were collected at 30-min intervals while RT were collected at 1-min intervals from -2 to 8 h relative to a lipopolysaccride (LPS) challenge (0.5 ìg/kg BW) at 0 h. Serum was analyzed for concentrations of cortisol and pro-inflammatory cytokines. All physical, endocrine, and immune measurements increased relative to LPS challenge. No differences observed for IL-6 or TNF-á; however, 1+QG heifers had a greater circulating INF-ã (P \u3c 0.001). Furthermore, 1+QG heifers had an elevated VT (P = 0.04). This would suggest a different immune system approach to an LPS challenge