Development of a microclip for laryngeal microsurgery: Initial animal studies

10.1002/lary.23280Laryngoscope12281809-1814LARY

In vivo efficacy of a novel liposomal formulation of safingol in the treatment of acute myeloid leukemia

10.1016/j.jconrel.2011.11.002Journal of Controlled Release1602290-298JCRE

Protective role of functionalized single walled carbon nanotubes enhance ex vivo expansion of hematopoietic stem and progenitor cells in human umbilical cord blood

10.1016/j.nano.2013.05.009Nanomedicine: Nanotechnology, Biology, and Medicine981304-131

Gut microbiota and Helicobacter pylori infections

Background: It has been widely accepted that Helicobacter pylori may be the only bacterium that can survive and infect the human stomach. Recently, a few studies using 16S rRNA clone library and other similar approaches showed that the gastric microbiome may be more complex than that. However, the role of interactions between H. pylori and other members of the gastric and gut microbiome in the development and progression of gastroduodenal diseases has not been extensively studied. Objective: The objective of this study is to establish the effects of gut microbiome microbiome in H. pylori infections using germ-free (GF) and specific pathogen-free (SPF) mice models. Methodology: Male GF and SPF C57BL/6 mice of 4–8 weeks of age were infected intragastrically with 109 CFU of rodent-adapted H. pylori strain 7.13 for 2, 8 and 16 weeks. At the end of the respective infection period, the animals were sacrificed. Stomach, liver and brain were harvested for microbiological and histopathological examinations. Synaptophysin and polysynaptic density protein 95 (PSD-95) levels in the brain were evaluated. Blood samples were collected for immunological and hormonal (leptin, total ghrelin and acyl ghrelin) analysis. Results: GF mouse model was established as an alternative animal model for studying H. pylori infections in a microbiota-free in vivo system. Discussion and conclusion: To further characterize the interplay between H. pylori, gut microflora and host, a meta-proteomics and meta-metabo-lomics approach will also be adopted. Outcome of this study will enhance our understanding of the pathogenesis of H. pylori-associated diseases in a systemic in vivo model against a complex multispecies environment

Pyrvinium selectively targets blast phase-chronic myeloid leukemia through inhibition of mitochondrial respiration

10.18632/oncotarget.5615Oncotarget63233769-3378

Adaptive Matrices and Filters for Color Texture Classification

<p>In this paper we introduce an integrative approach towards color texture classification and recognition using a supervised learning framework. Our approach is based on Generalized Learning Vector Quantization (GLVQ), extended by an adaptive distance measure, which is defined in the Fourier domain, and adaptive filter kernels based on Gabor filters. We evaluate the proposed technique on two sets of color texture images and compare results with those other methods achieve. The features and filter kernels learned by GLVQ improve classification accuracy and they are able to generalize much better for data previously unknown to the system.</p>

Bacterioplankton production in freshwater Antarctic lakes

1. Bacterioplankton production was measured in the water columns of two ultra-oligotrophic, freshwater Antarctic lakes (Crooked Lake and Lake Druzhby) during an annual cycle. In both lakes bacterial production, measured by the incorporation of [H-3] thymidine, continued in winter and showed a cycle over the year. The range of production was between 0 and 479 ng C L-1 h(-1) in Crooked Lake and 0-354 ng L-1 h(-1) in Lake Druzhby. 2. Abundance and mean cell volume both varied, producing marked changes in biomass during the year, with highest biomass occurring in the winter and early spring. Biomass showed similar seasonal trends in both lakes. 3. For most of the year inorganic forms of nitrogen and phosphorus were detectable in the water columns of the lakes and were unlikely to have limited bacterial production. Dissolved organic carbon (DOC) was below 3000 mug L-1. Dissolved amino acids and carbohydrates contributed 5-25% of the DOC pool in Crooked Lake and 5-64% in Lake Druzhby. Dissolved carbohydrates were consistently low, suggesting that this may have been the preferred carbon substrate for bacterioplankton. 4. Aggregate associated bacteria had higher mean cell volume, abundances and production than freely suspended bacteria in Lake Druzhby, while in Crooked Lake aggregate associated bacteria consistently had higher mean cell volumes than free bacteria, but abundance and production were on occasion higher in free bacteria compared with aggregate associated communities. 5. The data indicated that production is limited by continuous low temperatures and the limited availability of suitable DOC substrate. However, the bacterioplankton functions year round, responding to factors other than temperature

Genome-wide mutational signatures of aristolochic acid and its application as a screening tool

10.1126/scitranslmed.3006086Science Translational Medicine5197