Exceptionally large juvenile xanthogranuloma – a case report

Juvenile xanthogranuloma (JXG) is a rare, benign skin lesion pathologically classified as a non-Langerhans cell histiocytosis. The lesions appear within the first year of life in 75% of patients, predominantly on the head or neck, growing up to 5mm in size. While the etiology is mostly infectious, it can also be caused by genetic variants. In most patients, the condition has an easy course and resolution. Histopathological features include a histiocytic invasion of the superficial dermis, with additional eosinophils, lymphocytes and plasma cells. The lesion typically stains with anti-CD4, anti-XIIIa and CD68 markers

Prevalence of overweight and obesity in pediatric surgical population

Background: Obesity has become one of the most significant public health problems in the world in recent decades. It is associated with an increased incidence of postoperative complications, and there is a cause-and-effect relationship between obesity and increased prevalence of injury in children. Although there are studies in the world that indicate an increased incidence of obesity among surgically treated children, no such research has been conducted in Croatia so far. Materials and methods: This study aimed to determine the nutritional status of children treated at the Department of Pediatric Surgery at two samples in the time lag of eight years (2010 and 2018). In 2010, 1205 children were treated in hospital, 790 children fulfilled the inclusion criteria. In 2018, 1316 children were treated in hospital, and 790 children met the inclusion criteria. The collected data were compared with the existing health statistics. Results: The proportion of obese children was 29.7% in patients treated in 2010 and 29.7% in patients treated in 2018. In 2010 the highest prevalence of obese children was in pre-school age (23.8%) and overweight in school-age (16.7%). In 2018 the highest incidence of obesity in school children was 38.9%, in preschool-age 31.6%, in puberty 28.3%, and the lowest in adolescents 23.3%. Conclusion: The prevalence of obesity in children undergoing hospitalization for surgical illness is higher than in the general population. There was no trend of increasing the incidence of obesity over a 10-year time lag

Long-Term Assessment of Bioclimatic Conditions at Micro and Local Scales in the Cities of the Western Part of the Balkan Peninsula during the 21st Century

Thermal comfort assessments at local or micro-scales within urban areas can provide crucial insights for the urban adaptation strategies pertaining to climate-conscious urban planning and public health. However, the availability of long-term or mid-term daily or hourly meteorological data sets from urban environments remains a significant challenge even in the 21st century. Consequently, this study aimed to assess the thermal conditions in cities across the western part of the Balkan Peninsula, encompassing five countries (Slovenia, Croatia, Serbia, Bosnia and Herzegovina, and Montenegro), by utilizing the Physiological Equivalent Temperature (PET) index. Meteorological data sets, comprising air temperature, relative humidity, wind speed, and cloudiness, were collected from 32 national meteorological stations/measurement locations spanning the period from 2001 to 2020. The PET calculations were conducted based on meteorological data measured three times per day (7 a.m., 2 p.m., and 9 p.m.). Upon conducting a spatial analysis of the meteorological stations, it was observed that most of them (25 stations) were situated within built-up areas or urban suburbs, rendering them highly relevant for local or micro-scale climate and bioclimate assessments. The findings revealed that urban locations exhibited slightly higher PET heat stress levels, particularly during the summer season and at 2 p.m. Moreover, higher average PET values were observed in both urban and non-urban stations situated within a continental climate during warmer periods, such as summer. In contrast, during the colder seasons, namely winter and spring, higher PET values were prevalent in the Mediterranean region. Furthermore, the PET frequency analysis revealed a greater prevalence of extreme and severe heat stress levels in stations within continental climates, particularly those located in urban areas, as compared to stations in Mediterranean climates. In contrast, during the winter and spring seasons, monitoring stations in close proximity to the Adriatic Sea, characterized by a Mediterranean climate, exhibited significantly lower levels of cold stress compared to inland stations. Evidently, in addition to the climatic characteristics and surrounding terrain, the urban morphology significantly impacts the thermal conditions within cities

Population Pharmacogenomics in Croatia: Evaluating the PGx Allele Frequency and the Impact of Treatment Efficiency

Background: Adverse drug reactions (ADRs) are a significant cause of mortality, and pharmacogenomics (PGx) offers the potential to optimize therapeutic efficacy while minimizing ADRs. However, there is a lack of data on the Croatian population, highlighting the need for investigating the most common alleles, genotypes, and phenotypes to establish national guidelines for drug use. Methods: A single-center retrospective cross-sectional study was performed to examine the allele, genotype, and phenotype frequencies of drug-metabolizing enzymes, receptors, and other proteins in a random sample of 522 patients from Croatia using a 28-gene PGx panel. Results: Allele frequencies, genotypes, and phenotypes for the investigated genes were determined. No statistically significant differences were found between the Croatian and European populations for most analyzed genes. The most common genotypes observed in the patients resulted in normal metabolism rates. However, some genes showed higher frequencies of altered metabolism rates. Conclusions: This study provides insights into the allele, genotype, and phenotype frequencies of drug-metabolizing enzymes, receptors, and other associated proteins in the Croatian population. The findings contribute to optimizing drug use guidelines, potentially reducing ADRs, and improving therapeutic efficacy. Further research is needed to tailor population-specific interventions based on these findings and their long-term benefits

Implementing Whole Genome Sequencing (WGS) in Clinical Practice: Advantages, Challenges, and Future Perspectives

The integration of whole genome sequencing (WGS) into all aspects of modern medicine represents the next step in the evolution of healthcare. Using this technology, scientists and physicians can observe the entire human genome comprehensively, generating a plethora of new sequencing data. Modern computational analysis entails advanced algorithms for variant detection, as well as complex models for classification. Data science and machine learning play a crucial role in the processing and interpretation of results, using enormous databases and statistics to discover new and support current genotype–phenotype correlations. In clinical practice, this technology has greatly enabled the development of personalized medicine, approaching each patient individually and in accordance with their genetic and biochemical profile. The most propulsive areas include rare disease genomics, oncogenomics, pharmacogenomics, neonatal screening, and infectious disease genomics. Another crucial application of WGS lies in the field of multi-omics, working towards the complete integration of human biomolecular data. Further technological development of sequencing technologies has led to the birth of third and fourth-generation sequencing, which include long-read sequencing, single-cell genomics, and nanopore sequencing. These technologies, alongside their continued implementation into medical research and practice, show great promise for the future of the field of medicine

Unlocking the Potential of Mesenchymal Stem Cells in Gynecology: Where Are We Now?

Stem cells, with their remarkable capacity for differentiation into diverse cell types, are vital for the development as well as maintenance of health and homeostasis. Two unique abilities set them apart from other cells: self-renewal and the capacity for differentiation. They play important roles in embryogenesis, development, regeneration, and various other processes. Over the last decade, there has been increased interest in their potential use in the treatment of numerous diseases and disorders across multiple fields of medicine in acute, chronic, innate, and acquired diseases. Stem cells are key to maintaining the body’s homeostasis and regulating growth and tissue functions. There are several types of stem cells—embryonic, adult, and human-induced pluripotent cells. Currently, mesenchymal stem cells are of great interest due to their regenerative, immunomodulatory, analgesic, and antimicrobial (anti-inflammatory) effects. Recent studies have shown the potent regenerative effect of stem cell therapy in gynecologic diseases such as infertility, Asherman syndrome, lichen sclerosus, polycystic ovary syndrome, premature ovarian insufficiency, genitourinary syndrome of menopause, and rectovaginal fistulas. Moreover, the successful isolation of oogonial stem cells could lead to a revolution in the field of gynecology and the potential treatment of the conditions discussed. This review aims to provide a better understanding of the latest therapeutic options involving stem cells and raise awareness of this promising yet not widely known topic in gynecology and medicine in general