Dynamics of Skin Vessels Microcirculation Parameters in Rats at the Hypoxia

The work is devoted to the study of the effect of interval hypoxic training on the state of microcirculation of the skin in rats in the experiment. The study was conducted on white male Wistar rats weighing 70 g ±10%. In order to study the state of the dynamic characteristics of blood microcirculation, a change in blood perfusion of the tissue was considered. A non-invasive method for quantifying microvascular perfusion was used. The results showed that inhibition of microcirculation up to 50 % was observed in comparison to this value before exposure during the formation of a compensatory reaction of the body to a hypoxic effect on the 7–14th day of the experiment. It was noted that the predominant role in the regulation of the microvasculature belongs to the passive regulation factor of microcirculation which is characterized by the involvement of blood vessels in the microvasculature due to periodic changes in blood volume in the vessel together with an increase in the arithmetic mean perfusion rate. Thus, experimentally modulated hypobaric exposure, changes the volume of blood in arterioles that is determined by a pulse wave

Dynamics of Skin Vessels Microcirculation Parameters in Rats at the Hypoxia

The work is devoted to the study of the effect of interval hypoxic training on the state of microcirculation of the skin in rats in the experiment. The study was conducted on white male Wistar rats weighing 70 g ±10%. In order to study the state of the dynamic characteristics of blood microcirculation, a change in blood perfusion of the tissue was considered. A non-invasive method for quantifying microvascular perfusion was used. The results showed that inhibition of microcirculation up to 50 % was observed in comparison to this value before exposure during the formation of a compensatory reaction of the body to a hypoxic effect on the 7–14th day of the experiment. It was noted that the predominant role in the regulation of the microvasculature belongs to the passive regulation factor of microcirculation which is characterized by the involvement of blood vessels in the microvasculature due to periodic changes in blood volume in the vessel together with an increase in the arithmetic mean perfusion rate. Thus, experimentally modulated hypobaric exposure, changes the volume of blood in arterioles that is determined by a pulse wave

Neutrophils mediate pulmonary artery thrombosis in situ

Pulmonary embolism is a life-threatening condition, which can result in respiratory insufficiency and death. Blood clots occluding branches of the pulmonary artery (PA) are traditionally considered to originate from thrombi in deep veins (usually in legs). However, growing evidence suggests that occlusion of the vessels in the lungs can develop without preceding deep vein thrombosis (DVT). In this work, we used an inferior vena cava (IVC) complete ligation model of DVT in Wistar rats to explore the possibility and mechanisms of PA thrombosis under the conditions where all routes of thrombotic mass migration from peripheral veins are blocked. We demonstrate that rats both with normal and reduced neutrophil counts developed thrombi in the IVC, although, neutropenia caused a substantial decrease in thrombus size and a shift from fresh fibrin toward mature fibrin and connective tissue inside the thrombus. Massive fibrin deposition was found in the PA branches in the majority of DVT rats with normal neutrophil counts, but in none of the neutropenic animals. Neutrophil ablation also abolished macroscopic signs of lung damage. Altogether, the results demonstrate that thrombi in the lung vasculature can form in situ by mechanisms that require local neutrophil recruitment taking place in the DVT setting